Centrifugal seeding of mammalian cells in nonwoven fibrous matrices

Biotechnology Progress

Volumn 26, Issue 1, 2010, Pages 239-245

  Ng, Robin ^a   Gurm, Jesse Singh ^a   Yang, Shang Tian ^a  

^a Ohio State University   (United States)

Author keywords

Cell seeding; Centrifugation; Fibrous matrix; Polyethylene terephthalate; Tissue scaffold

Indexed keywords

3D SCAFFOLDS; BIOPROCESSING; CELL SEEDING; CELL VIABILITY; CELL-BASED ASSAYS; CENTRIFUGAL FORCES; COLON CANCER CELLS; FIBROUS MATRIX; FIBROUS SCAFFOLDS; HIGH-THROUGHPUT SCREENING; MAMMALIAN CELLS; NON-WOVEN; SEEDING EFFICIENCY; SEEDING METHOD; THREE-DIMENSIONAL (3D); TISSUE SCAFFOLDS;

ANIMAL CELL CULTURE; CELLS; CENTRIFUGATION; CENTRIFUGES; MAMMALS; POLYETHYLENE TEREPHTHALATES; POROSITY; SCAFFOLDS; THERMOPLASTICS; THREE DIMENSIONAL; TISSUE ENGINEERING; WEAVING;

CELL CULTURE;

POLYETHYLENE TEREPHTHALATE;

ARTICLE; CENTRIFUGATION; CHEMISTRY; CULTURE TECHNIQUE; EXTRACELLULAR MATRIX; HIGH THROUGHPUT SCREENING; HUMAN; METHODOLOGY; POROSITY; POSITRON EMISSION TOMOGRAPHY; SURFACE PROPERTY; TIME; TISSUE ENGINEERING; TUMOR CELL CULTURE;

CELL CULTURE TECHNIQUES; CENTRIFUGATION; EXTRACELLULAR MATRIX; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; POLYETHYLENE TEREPHTHALATES; POROSITY; POSITRON-EMISSION TOMOGRAPHY; SURFACE PROPERTIES; TIME FACTORS; TISSUE ENGINEERING; TUMOR CELLS, CULTURED;

MAMMALIA;

EID: 76849088789     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.317     Document Type: Article

References (39)

1. Basu S, Yang ST. Astrocyte growth and GDNF secretion in three dimensional polyethylene terephthalate fibrous matrices. Tissue Eng. 2005;11:940-952.
2. Ma T, Li Y, Yang ST, Kniss DA. Tissue engineering human placenta trophoblast cells in 3-D fibrous matrix: spatial effects on cell proliferation and functions. Biotechnol Prog. 1999; 15:715-724.
3. Yang Y, Basu S, Tomasko DL, Lee LJ, Yang ST. Fabrication of well defined PLGA scaffolds using novel microembossing and carbon dioxide bonding. Biomaterials. 2005;26:2585-2594.
4. Toh Y-C, Ho ST, Zhou Y, Hutmacher DW, Yu H. Application of a polyelectrolyte complex coacervation method to improve seeding efficiency of bone marrow stromal cells in a 3D culture system. Biomaterials. 2005;26:4149-4160.
5. Lehnart D, Wehrle-Haller B, David C, Weiland U, Ballestrem C, Imhof BA, Bastmeyer M. Cell behavior on micropatterned substrate: limits of extracellular matrix geometry for spreading and adhesion. J Cell Sci. 2003;117:41-52.
6. Orsello CE, Lauffenburger DA, Hammer DA. Molecular properties in cell adhesion: a physical and engineering perspective. Trends Biotechnol. 2001;19:310-316.
7. Xiao YL, Riesle J, Blitterswijk CA. Static seeding and dynamic fibroblast seeding and cultivation in porous PEO/PBT scaffold. J Mater Sci Mater Med. 1999;10:773-777.
8. Gooch KJ, Kwon JH, Blunk T, Langer BR, Freed LE, Vanjak-Novakovic G. Effects of mixing intensity on tissue-engineered cartilage. Biotechnol Bioeng. 2001;72:402-407.
9. Mascari L, Ymele-Leki P, Eggleton CD, Speziale P, Ross JM. Fluid shear contributions to bacteria cell detachment initiated by a monoclonal antibody. Biotechnol Bioeng. 2002;83:65-74.
10. Ouyang A, Yang ST. Effects of mixing intensity on cell seeding and proliferation in three-dimensional fibrous matrices. Biotechnol Bioeng. 2007;96:371-380.
11. Yang TH, Miyoshi H, Ohshima N. Novel cell immobilization method utilizing centrifugal force to achieve high-density hepatocyte culture in porous scaffold. J Biomed Mater Res. 2001;55:379-386.
12. Dar A, Shachar M, Leor J, Cohen J. Optimization of cardiac cell seeding and distribution in 3D porous alginate scaffolds. Biotechnol Bioeng. 2002;80:305-312.
13. Li Y, Ma T, Kniss DA, Lasky LC, Yang S-T. Effects of filtration seeding on cell density, spatial distribution and proliferation in nonwoven fibrous matrices. Biotechnol Prog. 2001;17:935-944.
14. Holy CE, Schoichet MS, Davies JE. Engineering three-dimensional bone tissue in vitro using biodegradable scaffolds: investigation initial cell seeding density and culture period. J Biomed Mater Res. 2000;51:376-382.
15. Gao J, Niklason L, Langer R. Surface hydrolysis of poly(glycolic acid) meshes increases the seeding density of vascular smooth muscle cells. J Biomed Mater Res. 1998;42:417-423.
16. Kim B-S, Putnam AJ, Kulik TJ, Mooney DJ. Optimizing seeding and culture methods to engineer sooth muscle tissue on biodegradable polymer matrices. Biotechnol Bioeng. 1998;57:46-54.
17. Nasseri BA, Pomerantseva I, Kaazempur-Mofrard MR, Sutherland FWH, Perry T, Ochoa E, Thompson CA, Mayer JE, Oesterle SN, Vacanti JP. Dynamic rotational seeding and cell culture system for vascular tube formation. Tissue Eng. 2003;9: 291-299.
18. Zhao F, Ma T. Perfusion bioreactor system for human mesenchymal stem cell tissue engineering: dynamic cell seeding and construct development. Biotechnol Bioeng. 2005;91:482-493.
19. Schreiber RE, Dunkelman NS, Naughton G, Ratcliffe A. A method for tissue engineering of cartilage by cell seeding on bioresorbable scaffolds. Ann NY Acad Sci. 1999;875:398-404.
20. Conklin BS, Wu H, Lin PH, Lumsden AB, Chen C. Basic fibroblast growth factor coating and endothelial cell seeding of a decellularized heparin-coated vascular graft. Artif Organs. 2004;28:668-675.
21. Wendt D, Marsano A, Jakob M, Hebere M, Martin I. Oscillating perfusion of cell suspensions through three dimensional scaffolds enhances cell seeding efficiency and uniformity. Biotechnol Bioeng. 2003;84:205-214.
22. Noah EM, Chen J, Jiao X, Heschel I, Pallia N. Impact of sterilization on the porous design and cell behavior in collagen sponges prepared for tissue engineering. Biomaterials. 2002;23: 2855-2861.
23. Vunjak-Novakovic G, Obradovic B, Martin I, Bursac PM, Langer R, Freed LE. Dynamic cell seeding of polymer scaffolds for cartilage tissue engineering. Biotechnol Prog. 1998;14:193-202.
24. Tan WJ, Teo GP, Leong KW, Mao H-Q, Chan V. Adhesion contact dynamics of primary hepatocytes on poly(ethylene terephthalate) surface. Biomaterials. 2005;26:891-898.
25. Wissink MJB, Beernink R, Scharenborg NM, Poot AA, Engbers GHM, Beugeling T, Van Aken WG, Feijen J. Endothelial cell seeding of (heparinized) collagen matrices: effects of bFGF preloading on proliferation (after low density seeding) and procoagulant factors. J Control Release. 2000;67:141-155.
26. Yang ST, Luo J, Chen C. A fibrous-bed bioreactor for continuous production of monoclonal antibody by hybridoma. In: Zhong JJ, editor. Advances in Biochemical Engineering/Biotechnology, Vol. 87: Biomanufacturing. Heidelberg: Springer-Verlag; 2004:61-96.
27. Ma T, Li Y, Yang ST, Kniss DA. Effects of pore size on 3-D fibrous matrices on human trophoblast tissue development. Biotechnol Bioeng. 2000;70:606-618.
28. Li Y, Ma T, Yang ST, Kniss DA. Thermal compression and characterization of three-dimensional nonwoven PET matrices as tissue engineering scaffolds. Biomaterials. 2001;22:609-618.
29. Ouyang A, Ng R, Yang ST. Long-term culturing of undifferentiated embryonic stem cells in conditioned media and three-dimensional fibrous matrices without ECM coating. Stem Cells. 2007;25:447-454.
30. Mehta B, Holman DW, Grzybowski DM, Chalmers JJ. Characterization of arachnoidal cells cultured on three-dimensional nonwoven PET matrix. Tissue Eng. 2007;13:1-11.
31. Li Y, Yang ST. Effects of three-dimensional scaffolds on cell organization and tissue development. Biotechnol Bioprocess Eng. 2001;6:311-325.
32. Luo J, Yang ST. Effects of three-dimensional culturing in a fibrous matrix on cell cycle, apoptosis, and mAb production by hybridoma Cells. Biotechnol Prog. 2004;20:306-315.
33. Grayson WL, Ma T, Bunnell B. Human mesenchymal stem cells tissue development in 3D PET Matrices. Biotechnol Prog. 2004;20:905-912.
34. Yasuka K, Inoue S, Tabata Y. Influence of culture method on the proliferation and osteogenic differentiation of human adipostromal cells in nonwoven fabrics. Tissue Eng. 2004;10:1587-1596.
35. Kunz-Schughart LA, Freyer JP, Hofstaedter F, Ebner R. The use of 3-D cultures for high-throughput screening: the multicellular spheroid model. J Biomol Screening. 2004;9:273-285.
36. Yang S-T, Zhang X, Wen Y. Microbioreactors for high-throughput cytotoxicity assays. Curr Opin Drug Discovery Dev. 2008;11:111-127.
37. Wen Y, Yang S-T. The future of microfluidic assays in drug development. Expert Opin Drug Discovery. 2008;3:1237-1253.
38. Zhang X. Three-Dimensional High-Throughput Screening for Drug Discovery and Cell Culture Process Development, PhD Dissertation. Ohio: The Ohio State University; 2008:9-45.
39. Wen Y. Microfluidic and Microscale Cell Culture for High-Throughput Cell-Based Assays and Bioprocess Development, PhD Dissertation. Ohio: The Ohio State University; 2009:76-92.