High-level soluble expression, purification and characterization of active human midkine from Escherichia coli

Protein Expression and Purification

Volumn 70, Issue 2, 2010, Pages 270-276

  Kelly Yan, W ^a   Goette, Marjo ^b   Hofmann, Gabriele ^b   Zaror, Isabel ^a   Sim, Janet ^a  

^a NOVARTIS INSTITUTES FOR BIOMEDICAL RESEARCH   (United States)

^b NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Affinity purification; Analysis; Disulfide bond; Escherichia coli expression; Human midkine

Indexed keywords

HYBRID PROTEIN; MDK PROTEIN, HUMAN; NERVE GROWTH FACTOR;

ARTICLE; BIOSYNTHESIS; CEREBELLUM; CYTOLOGY; DRUG EFFECT; ESCHERICHIA COLI; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; NEURITE;

CEREBELLUM; ESCHERICHIA COLI; HUMANS; NERVE GROWTH FACTORS; NEURITES; RECOMBINANT FUSION PROTEINS;

ESCHERICHIA COLI; RATTUS;

EID: 76749168492     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.10.015     Document Type: Article

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