Improved methods for expression and purification of Saccharomyces cerevisiae TFIIF and TFIIH; Identification of a functional Escherichia coli promoter and internal translation initiation within the N-terminal coding region of the TFIIF TFG1 subunit

Protein Expression and Purification

Volumn 70, Issue 2, 2010, Pages 172-178

  Yang, Chen ^a   Khaperskyy, Denys A ^a   Hou, Min ^a   Ponticelli, Alfred S ^a  

^a UNIVERSITY AT BUFFALO   (United States)

Author keywords

S. cerevisiae; TFIIF; TFIIH; Transcription

Indexed keywords

PROTEIN SUBUNIT; RECOMBINANT PROTEIN; TRANSCRIPTION FACTOR II; TRANSCRIPTION FACTOR IIH; TRANSCRIPTION FACTOR TFIIF;

AFFINITY CHROMATOGRAPHY; ARTICLE; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYSIOLOGY; PROMOTER REGION; RNA TRANSLATION; SACCHAROMYCES CEREVISIAE;

BASE SEQUENCE; CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; PEPTIDE CHAIN INITIATION, TRANSLATIONAL; PROMOTER REGIONS, GENETIC; PROTEIN SUBUNITS; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; TRANSCRIPTION FACTOR TFIIH; TRANSCRIPTION FACTORS, TFII;

ESCHERICHIA COLI; SACCHAROMYCES CEREVISIAE; SACCHAROMYCETALES;

EID: 76749161273     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.09.021     Document Type: Article

References (42)

1. Flores O., Lu H., Killeen M., Greenblatt J., Burton Z.F., and Reinberg D. The small subunit of transcription factor IIF recruits RNA polymerase II into the preinitiation complex. Proc. Natl. Acad. Sci. USA 88 (1991) 9999-10003
2. Maxon M.E., Goodrich J.A., and Tjian R. Transcription factor IIE binds preferentially to RNA polymerase IIa and recruits TFIIH: a model for promoter clearance. Genes Dev. 8 (1994) 515-524
3. Robert F., Douziech M., Forget D., Egly J.M., Greenblatt J., Burton Z.F., and Coulombe B. Wrapping of promoter DNA around the RNA polymerase II initiation complex induced by TFIIF. Mol. Cell 2 (1998) 341-351
4. Ghazy M.A., Brodie S.A., Ammerman M.L., Ziegler L.M., and Ponticelli A.S. Amino acid substitutions in yeast TFIIF confer upstream shifts in transcription initiation and altered interaction with RNA polymerase II. Mol. Cell. Biol. 24 (2004) 10975-10985
5. Sun Z.W., and Hampsey M. Identification of the gene (SSU71/TFG1) encoding the largest subunit of transcription factor TFIIF as a suppressor of a TFIIB mutation in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 92 (1995) 3127-3131
6. Yan Q., Moreland R.J., Conaway J.W., and Conaway R.C. Dual roles for transcription factor IIF in promoter escape by RNA polymerase II. J. Biol. Chem. 274 (1999) 35668-35675
7. Khaperskyy D.A., Ammerman M.L., Majovski R.C., and Ponticelli A.S. Functions of Saccharomyces cerevisiae TFIIF during transcription start site utilization. Mol. Cell. Biol. 28 (2008) 3757-3766
8. Tan S., Aso T., Conaway R.C., and Conaway J.W. Roles for both the RAP30 and RAP74 subunits of transcription factor IIF in transcription initiation and elongation by RNA polymerase II. J. Biol. Chem. 269 (1994) 25684-25691
9. Izban M.G., and Luse D.S. Factor-stimulated RNA polymerase II transcribes at physiological elongation rates on naked DNA but very poorly on chromatin templates. J. Biol. Chem. 267 (1992) 13647-13655
10. Zhang C., and Burton Z.F. Transcription factors IIF and IIS and nucleoside triphosphate substrates as dynamic probes of the human RNA polymerase II mechanism. J. Mol. Biol. 342 (2004) 1085-1099
11. Chambers R.S., Wang B.Q., Burton Z.F., and Dahmus M.E. The activity of COOH-terminal domain phosphatase is regulated by a docking site on RNA polymerase II and by the general transcription factors IIF and IIB. J. Biol. Chem. 270 (1995) 14962-14969
12. Svejstrup J.Q., Vichi P., and Egly J.M. The multiple roles of transcription/repair factor TFIIH. Trends Biochem. Sci. 21 (1996) 346-350
13. Schaeffer L., Roy R., Humbert S., Moncollin V., Vermeulen W., Hoeijmakers J.H., Chambon P., and Egly J.M. DNA repair helicase: a component of BTF2 (TFIIH) basic transcription factor. Science 260 (1993) 58-63
14. Guzder S.N., Sung P., Bailly V., Prakash L., and Prakash S. RAD25 is a DNA helicase required for DNA repair and RNA polymerase II transcription. Nature 369 (1994) 578-581
15. Sung P., Prakash L., Matson S.W., and Prakash S. RAD3 protein of Saccharomyces cerevisiae is a DNA helicase. Proc. Natl. Acad. Sci. USA 84 (1987) 8951-8955
16. Sung P., Prakash L., Weber S., and Prakash S. The RAD3 gene of Saccharomyces cerevisiae encodes a DNA-dependent ATPase. Proc. Natl. Acad. Sci. USA 84 (1987) 6045-6049
17. Sung P., Bailly V., Weber C., Thompson L.H., Prakash L., and Prakash S. Human xeroderma pigmentosum group D gene encodes a DNA helicase. Nature 365 (1993) 852-855
18. Bootsma D., and Hoeijmakers J.H. DNA repair. Engagement with transcription. Nature 363 (1993) 114-115
19. Schaeffer L., Moncollin V., Roy R., Staub A., Mezzina M., Sarasin A., Weeda G., Hoeijmakers J.H., and Egly J.M. The ERCC2/DNA repair protein is associated with the class II BTF2/TFIIH transcription factor. EMBO J. 13 (1994) 2388-2392
20. Feaver W.J., Svejstrup J.Q., Henry N.L., and Kornberg R.D. Relationship of CDK-activating kinase and RNA polymerase II CTD kinase TFIIH/TFIIK. Cell 79 (1994) 1103-1109
21. Lu H., Zawel L., Fisher L., Egly J.M., and Reinberg D. Human general transcription factor IIH phosphorylates the C-terminal domain of RNA polymerase II. Nature 358 (1992) 641-645
22. Svejstrup J.Q., Wang Z., Feaver W.J., Wu X., Bushnell D.A., Donahue T.F., Friedberg E.C., and Kornberg R.D. Different forms of TFIIH for transcription and DNA repair: holo-TFIIH and a nucleotide excision repairosome. Cell 80 (1995) 21-28
23. Feaver W.J., Huang W., Gileadi O., Myers L., Gustafsson C.M., Kornberg R.D., and Friedberg E.C. Subunit interactions in yeast transcription/repair factor TFIIH. Requirement for Tfb3 subunit in nucleotide excision repair. J. Biol. Chem. 275 (2000) 5941-5946
24. Coin F., Oksenych V., Mocquet V., Groh S., Blattner C., and Egly J.M. Nucleotide excision repair driven by the dissociation of CAK from TFIIH. Mol. Cell 31 (2008) 9-20
25. Henry N.L., Campbell A.M., Feaver W.J., Poon D., Weil P.A., and Kornberg R.D. TFIIF-TAF-RNA polymerase II connection. Genes Dev. 8 (1994) 2868-2878
26. Cairns B.R., Henry N.L., and Kornberg R.D. TFG/TAF30/ANC1, a component of the yeast SWI/SNF complex that is similar to the leukemogenic proteins ENL and AF-9. Mol. Cell. Biol. 16 (1996) 3308-3316
27. Treich I., and Carlson M. Interaction of a Swi3 homolog with Sth1 provides evidence for a Swi/Snf-related complex with an essential function in Saccharomyces cerevisiae. Mol. Cell. Biol. 17 (1997) 1768-1775
28. Eberharter A., John S., Grant P.A., Utley R.T., and Workman J.L. Identification and analysis of yeast nucleosomal histone acetyltransferase complexes. Methods 15 (1998) 315-321
29. Steger D.J., Eberharter A., John S., Grant P.A., and Workman J.L. Purified histone acetyltransferase complexes stimulate HIV-1 transcription from preassembled nucleosomal arrays. Proc. Natl. Acad. Sci. USA 95 (1998) 12924-12929
30. John S., Howe L., Tafrov S.T., Grant P.A., Sternglanz R., and Workman J.L. The something about silencing protein, Sas3, is the catalytic subunit of NuA3, a yTAF(II)30-containing HAT complex that interacts with the Spt16 subunit of the yeast CP (Cdc68/Pob3)-FACT complex. Genes Dev. 14 (2000) 1196-1208
31. Shen X., Mizuguchi G., Hamiche A., and Wu C. A chromatin remodelling complex involved in transcription and DNA processing. Nature 406 (2000) 541-544
32. Kabani M., Michot K., Boschiero C., and Werner M. Anc1 interacts with the catalytic subunits of the general transcription factors TFIID and TFIIF, the chromatin remodeling complexes RSC and INO80, and the histone acetyltransferase complex NuA3. Biochem. Biophys. Res. Commun. 332 (2005) 398-403
33. Takagi Y., Komori H., Chang W.H., Hudmon A., Erdjument-Bromage H., Tempst P., and Kornberg R.D. Revised subunit structure of yeast transcription factor IIH (TFIIH) and reconciliation with human TFIIH. J. Biol. Chem. 278 (2003) 43897-43900
34. Ranish J.A., Hahn S., Lu Y., Yi E.C., Li X.J., Eng J., and Aebersold R. Identification of TFB5, a new component of general transcription and DNA repair factor IIH. Nat. Genet. 36 (2004) 707-713
35. Giglia-Mari G., Coin F., Ranish J.A., Hoogstraten D., Theil A., Wijgers N., Jaspers N.G., Raams A., Argentini M., van der Spek P.J., Botta E., Stefanini M., Egly J.M., Aebersold R., Hoeijmakers J.H., and Vermeulen W. A new, tenth subunit of TFIIH is responsible for the DNA repair syndrome trichothiodystrophy group A. Nat. Genet. 36 (2004) 714-719
36. Svejstrup J.Q., Feaver W.J., LaPointe J., and Kornberg R.D. RNA polymerase transcription factor IIH holoenzyme from yeast. J. Biol. Chem. 269 (1994) 28044-28048
37. Myers L.C., Leuther K., Bushnell D.A., Gustafsson C.M., and Kornberg R.D. Yeast RNA polymerase II transcription reconstituted with purified proteins. Methods 12 (1997) 212-216
38. Rani P.G., Ranish J.A., and Hahn S. RNA polymerase II (Pol II)-TFIIF and Pol II-mediator complexes: the major stable Pol II complexes and their activity in transcription initiation and reinitiation. Mol. Cell. Biol. 24 (2004) 1709-1720
39. Ziegler L.M., Khaperskyy D.A., Ammerman M.L., and Ponticelli A.S. Yeast RNA polymerase II lacking the Rpb9 subunit is impaired for interaction with transcription factor IIF. J. Biol. Chem. 278 (2003) 48950-48956
40. Gerischer U., and Durre P. MRNA analysis of the adc gene region of Clostridium acetobutylicum during the shift to solventogenesis. J. Bacteriol. 174 (1992) 426-433
41. Ponticelli A.S., and Struhl K. Analysis of Saccharomyces cerevisiae his3 transcription in vitro: Biochemical support for multiple mechanisms of transcription. Mol. Cell. Biol. 10 (1990) 2832-2839
42. Wang B.Q., and Burton Z.F. Functional domains of human RAP74 including a masked polymerase binding domain. J. Biol. Chem. 270 (1995) 27035-27044