Primary prostate cancer cultures are models for androgen-independent transit amplifying cells

Oncology Reports

Volumn 23, Issue 2, 2010, Pages 465-470

  Bühler, Patrick ^a   Wolf, Philipp ^a   Katzenwadel, Arndt ^a   Schultze Seemann, Wolfgang ^a   Wetterauer, Ulrich ^a   Freudenberg, Nikolaus ^a   Elsässer Beile, Ursula ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

Author keywords

Androgen receptor; Androgen relevant genes; Basal cells; Cytokeratin; Luminal cells; Prostate cancer cultures; Transit amplifying cells

Indexed keywords

ANDROGEN RECEPTOR; ANDROSTANOLONE; CYTOKERATIN 5; KALLIKREIN; KALLIKREIN 2; MATRIGEL; PROSTATE SPECIFIC ANTIGEN; PROSTATE SPECIFIC MEMBRANE ANTIGEN; PROSTATE STEM CELL ANTIGEN; RNA; STEROID 5ALPHA REDUCTASE INHIBITOR; UNCLASSIFIED DRUG;

ARTICLE; CANCER CELL CULTURE; CELL GROWTH; CELL STRUCTURE; CLINICAL ARTICLE; CONTROLLED STUDY; GENE EXPRESSION; HUMAN; HUMAN CELL; HUMAN TISSUE; MALE; MARKER GENE; MONOLAYER CULTURE; PRIORITY JOURNAL; PROSTATE CANCER; PROSTATE EPITHELIUM; PROSTATECTOMY; RNA ISOLATION;

ANDROGEN ANTAGONISTS; ANDROGENS; CARCINOMA; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL PROLIFERATION; EPITHELIAL CELLS; GENE EXPRESSION REGULATION, NEOPLASTIC; HUMANS; MALE; MEMBRANE GLYCOPROTEINS; MODELS, BIOLOGICAL; NEOPLASM PROTEINS; PHENOTYPE; PROSTATIC NEOPLASMS; RECEPTORS, ANDROGEN; TUMOR CELLS, CULTURED; TUMOR MARKERS, BIOLOGICAL;

EID: 76649141228     PISSN: 1021335X     EISSN: 17912431     Source Type: Journal    
DOI: 10.3892/or-00000656     Document Type: Article

References (22)

1. Mitchell S, Abel P, Ware M, Stamp G and Lalani E: Phenotypic and genotypic characterization of commonly used human prostatic cell lines. BJU Int 85: 932-944, 2000.
2. Peehl DM and Sellers RG: Epithelial cell culture: prostate. In: Methods of Tissue Engineering. Atala A and Lanza RP (eds). Academic Press, San Diego, pp247-261, 2002.
3. Peehl DM: Primary cell cultures as models of prostate cancer development. Endocr Relat Cancer 12: 19-47, 2005.
4. Tran CP, Lin C, Yamashiro J and Reiter RE: Prostate stem cell antigen is a marker of late intermediate prostate epithelial cells. Mol Cancer Res 1: 113-121, 2002.
5. Coffey RN, Watson RW, Hegarty PK, Watson CL, Wolohan L, Brady HR, O'Keane C and Fitzpatrick JM: Priming prostate carcinoma cells for increased apoptosis is associated with upregulation of the caspases. Cancer 92: 2297-2308, 2001.
6. Peehl DM: Are primary cultures realistic models of prostate cancer? J Cell Biochem 91: 185-195, 2004.
7. Lang SH, Stark M, Collins A, Paul AB, Stower MJ and Maitland NJ: Experimental prostate epithelial morphogenesis in response to stroma and three-dimensional matrigel culture. Cell Growth Differ 12: 631-640, 2001.
8. Hall JA, Maitland NJ, Stower M and Lang SH: Primary prostate stromal cells modulate the morphology and migration of primary prostate epithelial cells in type 1 collagen gels. Cancer Res 62: 58-62, 2002.
9. Cunha GR, Hayward SW, Wang YZ and Ricke WA: Role of the stromal microenvironment in carcinogenesis of the prostate. Int J Cancer 107: 1-10, 2003.
10. Barclay WW, Woodruff RD, Hall MC and Cramer SD: A system for studying epithelial-stromal interactions reveals distinct inductive abilities of stromal cells from benign prostatic hyperplasia and prostate cancer. Endocrinology 146: 13-18, 2005.
11. Zwergel T, Kakirman H, Schorr H, Wullich B and Unteregger G: A new serial transfer explant cell culture system for human prostatic cancer tissues preventing selection toward diploid cells. Cancer Genet Cytogenet 101: 16-23, 1998.
12. Castellon E, Venegas K, Saenz L, Contreras H and Huidobro C: Secretion of prostatic specific antigen, proliferative activity and androgen response in epithelial-stromal co-cultures from human prostate carcinoma. Int J Androl 28: 39-46, 2005.
13. Bonkhoff H, Stein U and Remberger K: Multidirectional differentiation in the normal, hyperplastic, and neoplastic human prostate: simultaneous demonstration of cell-specific epithelial markers. Hum Pathol 25: 42-46, 1994.
14. Garraway LA, Lin D, Signoretti S, Waltregny D, Dilks J, Bhattacharya N and Loda M: Intermediate basal cells of the prostate: in vitro and in vivo characterization. Prostate 55: 206-218, 2003.
15. Uzgare AR, Xu Y and Isaacs JT: In vitro culturing and characteristics of transit amplifying epithelial cells from human prostate tissue. J Cell Biochem 91: 196-205, 2004.
16. Festuccia C, Angelucci A, Gravina GL, Muzi P, Miano R, Vicentini C and Bologna M: Epithelial and prostatic marker expression in short-term primary cultures of human prostate tissue samples. Int J Oncol 26: 1353-1362, 2005.
17. Festuccia C, Angelucci A, Gravina GL, Muzi P, Vicentini C and Bologna M: Effects of 5 alpha reductase inhibitors on androgen-dependent human prostatic carcinoma cells. J Cancer Res Clin Oncol 131: 243-254, 2005.
18. Gravina GL, Festuccia C, Millimaggi D, Tombolini V, Dolo V, Vicentini C and Bologna M: Bicalutamide demonstrates biologic effectiveness in prostate cancer cell lines and tumor primary cultures irrespective of Her2/neu expression levels. Urology 74: 452-457, 2009.
19. Nanni S, Priolo C, Grasselli A, D'Eletto M, Merola R, Moretti F, Gallucci M, De Carli P, Sentinelli S, Cianciulli AM, Mottolese M, Carlini P, Arcelli D, Helmer-Citterich M, Gaetano C, Loda M, Pontecorvi A, Bacchetti S, Sacchi A and Farsetti A: Epithelial-restricted gene profile of primary cultures from human prostate tumors: a molecular approach to predict clinical behavior of prostate cancer. Mol Cancer Res 4: 79-92, 2006.
20. Kirschenbaum A, Liu XH, Yao S, Narla G, Friedman SL, Martignetti JA and Levine AC: Sex steroids have differential effects on growth and gene expression in primary human prostatic epithelial cell cultures derived from the peripheral versus transition zones. Carcinogenesis 27: 216-224, 2006.
21. Peehl DM, Shinghal R, Nonn L, Seto E, Krishnan AV, Brooks JD and Feldman D: Molecular activity of 1,25-dihydroxyvitamin D3 in primary cultures of human prostatic epithelial cells revealed by cDNA microarray analysis. J Steroid Biochem Mol Biol 92: 131-141, 2004.
22. Rose A, Xu Y, Chen Z, Fan Z, Stamey TA, McNeal JE, Caldwell M and Peehl DM: Comparative gene and protein expression in primary cultures of epithelial cells from benign prostatic hyperplasia and prostate cancer. Cancer Lett 227: 213-222, 2005.