Improved electrotransformation and decreased antibiotic resistance of the cystic fibrosis pathogen burkholderia cenocepacia strain J2315

Applied and Environmental Microbiology

Volumn 76, Issue 4, 2010, Pages 1095-1102

  Dubarry, Nelly ^a,b   Du, Wenli ^a   Lane, David ^a   Pasta, Franck ^a  

^a CNRS   (France)

^b UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC RESISTANCE; ANTIBIOTIC RESISTANCE GENES; BURKHOLDERIA; CYSTIC FIBROSIS; EFFLUX PUMPS; ELECTROPORATION; ELECTROTRANSFORMATION;

GENES;

ANTIBIOTICS;

BACTERIAL DNA; GLYCINE; PRIMER DNA; SPERMINE;

ANTIBIOTIC RESISTANCE; BACTERIUM; CHROMOSOME; EPIDEMIOLOGY; GENOME; PATHOGENICITY;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BURKHOLDERIA CEPACIA COMPLEX; CHEMISTRY; CULTURE MEDIUM; CYSTIC FIBROSIS; DNA METHYLATION; DRUG EFFECT; ELECTROPORATION; GENETIC RECOMBINATION; GENETIC TRANSFORMATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MUTATION; NUCLEOTIDE SEQUENCE; PATHOGENICITY; PLASMID;

BASE SEQUENCE; BURKHOLDERIA CEPACIA COMPLEX; CULTURE MEDIA; CYSTIC FIBROSIS; DNA METHYLATION; DNA PRIMERS; DNA, BACTERIAL; DRUG RESISTANCE, BACTERIAL; ELECTROPORATION; GENES, BACTERIAL; GLYCINE; HUMANS; MICROBIAL SENSITIVITY TESTS; MUTATION; PLASMIDS; RECOMBINATION, GENETIC; SPERMINE; TRANSFORMATION, GENETIC;

BACTERIA (MICROORGANISMS); BURKHOLDERIA CENOCEPACIA;

EID: 76649123605     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02123-09     Document Type: Article

References (42)

1. 1. Atanasiu, C., O. Byron, H. McMiken, S. S. Sturrock, and D. T. Dryden. 2001. Characterisation of the structure of Ocr, the gene 0.3 protein of bacteriophage T7. Nucleic Acids Res. 29:3059-3068. (Pubitemid 32685232)
2. Bolivar, F. 1978. Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique EcoRI sites for selection of EcoRI generated recombinant DNA molecules. Gene 4:121-136.
3. Burkholder, W. H. 1950. Sour skin, a bacterial rot of onion bulbs. Phytopathology 40:115-117.
4. Burns, J. L., and L. A. Hedin. 1991. Genetic transformation of Pseudomonas cepacia using electroporation. J. Microbiol. Methods 13:215-221.
5. Dela Vega, A. L., and A. H. Delcour. 1996. Polyamines decrease Escherichia coli outer membrane permeability. J. Bacteriol. 178:3715-3721.
6. Dubarry, N., F. Pasta, and D. Lane. 2006. ParABS systems of the four replicons of Burkholderia cenocepacia: new chromosome centromeres confer partition specificity. J. Bacteriol. 188:1489-1496.
7. Engledow A. S., E. G. Medrano, E. Mahenthiralingam, J. J. LiPuma, and C. F. Gonzalez. 2004. Involvement of a plasmid-encoded type IV secretion system in the plant tissue watersoaking phenotype of Burkholderia cenocepacia. J. Bacteriol. 186:6015-6024.
8. Figurski, D. H., and D. R. Helinski. 1979. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc. Natl. Acad. Sci. U. S. A. 76:1648-1652.
9. Flannagan, R. S., T. Linn, and M. A. Valvano. 2008. A system for the construction of targeted unmarked gene deletions in the genus Burkholderia. Environ. Microbiol. 10:1652-1660.
10. Grant, S. G., J. Jessee, F. R. Bloom, and D. Hanahan. 1990. Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants. Proc. Natl. Acad. Sci. U. S. A. 87:4645-4649.
11. Guglierame, P., M. R. Pasca, E. De Rossi, S. Buroni, P. Arrigo, G. Manina, and G. Riccardi. 2006. Efflux pump genes of the resistance- nodulationdivision family in Burkholderia cenocepacia genome. BMC Microbiol. 6:66.
12. Gugliucci, A. 2004. Polyamines as clinical laboratory tools. Clin. Chim. Acta 344:23-35.
13. Helmark, S., M. E. Hansen, B. Jelle, K. I. Sørensen, and P. R. Jensen. 2004. Transformation of Leuconostoc carnosum 4010 and evidence for natural competence of the organism. Appl. Environ. Microbiol. 70:3695-3699.
14. Hoang, T. T., R R. Karkhoff-Schweizer, A. J. Kutchma, and H. P. Schweizer. 1998. A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants. Gene 212:77-86.
15. Holden, M. T. G., H. M. B. Seth-Smith, L. C. Crossman, M. Sebaihia, S. D. Bentley, A. M. Cerdepo-Tárraga, N. R Thomson, N. Bason, M. A. Quail, S. Sharp, I. Cherevach, C. Churcher, I. Goodhead, H. Hauser, N. Holroyd, K. Mungall, P. Scott, D. Walker, B. White, H. Rose, P. Iversen, D. Mil-Homens, E. P. C. Rocha, A. M. Fialho, A. Baldwin, C. Dowson, B. G. Barrell, J. R. Govan, P. Vandamme, C. A. Hart, E. Mahenthiralingam, and J. Parkhill. 2009. The genome of Burkholderia cenocepacia J2315, an epidemic pathogen of cystic fibrosis patients. J. Bacteriol. 191:261-277.
16. Holo, H., and I. F. Nes. 1989. High-frequency transformation, by electroporation, of Lactococcus lactis subsp. cremoris grown with glycine in osmotically stabilized media. Appl. Environ. Microbiol. 55:3119-3123.
17. Hunt, T. A., C. Kooi, P. A. Sokol, and M. A. Valvano. 2004. Identification of Burkholderia cenocepacia genes required for bacterial survival in vivo. Infect. Immun. 72:4010-4022.
18. Igarashi, K., and K. Kashiwagi. 2000. Polyamines: mysterious modulators of cellular functions. Biochem. Biophys. Res. Commun. 271:559-564.
19. Isles, A., I. Maclusky, M. Corey, R Gold, C. Prober, P. Fleming, and H. Levison. 1984. Pseudomonas cepacia infection in cystic fibrosis: an emerging problem. J. Pediatr. 104:206-210.
20. Johnson, W. M., S. D. Tyler, and K. R. Rozee. 1994. Linkage analysis of geographic and clinical clusters in Pseudomonas cepacia infections by multilocus enzyme electrophoresis and ribotyping. J. Clin. Microbiol. 32:924-930.
21. Kovach, M. E., P. H. Elzer, D. S. Hill, G. T. Robertson, M. A. Farris, R. M. Roop, and K. M. Peterson. 1995. Four new derivatives of the broad-hostrange cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 166:175-176.
22. Kwon, D. H., and C. Lu. 2006. Polyamines induce resistance to cationic peptide, aminoglycoside, and quinolone antibiotics in Pseudomonas aeruginosa PAO1. Antimicrob. Agents Chemother. 50:1615-1622.
23. Kwon, D.-H., and C. Lu. 2007. Polyamine effects on antibiotic susceptibility in bacteria. Antimicrob. Agents Chemother. 51:2070-2077.
24. Lefebre, M. D., and M. A. Valvano. 2002. Construction and evaluation of plasmid vectors optimized for constitutive and regulated gene expression in Burkholderia cepacia complex isolates. Appl. Environ. Microbiol. 68:5956-5964.
25. Lessie, T. G., W. Hendrickson, B. D. Manning, and R. Devereux. 1996. Genomic complexity and plasticity of Burkholderia cepacia. FEMS Microbiol. Lett. 144:117-128.
26. Mahenthiralingam, E., T. Coenye, J. W. Chung, D. P. Speert, J. R Govan, P. Taylor, and P. Vandamme. 2000. Diagnostically and experimentally useful panel of strains from the Burkholderia cepacia complex. J. Clin. Microbiol. 38:910-913.
27. Mahenthiralingam, E., T. A. Urban, and J. B. Goldberg. 2005. The multifarious, multireplicon Burkholderia cepacia complex. Nat. Rev. Microbiol. 3:144-156.
28. 28. Mark, K. K., and F. W. Studier. 1981. Purification of the gene 0.3 protein of bacteriophage T7, an inhibitor of the DNA restriction system of Escherichia coli. J. Biol. Chem. 256:2573-2578. (Pubitemid 11103300)
29. Marx, C. J., and M. E. Lidstrom. 2002. Broad-host-range cre-lox system for antibiotic marker recycling in gram-negative bacteria. Biotechniques 33: 1062-1067.
30. Morales, V. M., A. Bäckman, and M. Bagdasarian. 1991. A series of widehost-range low-copy-number vectors that allow direct screening for recombinants. Gene 97:39-47.
31. Nzula, S., P. Vandamme, and J. R W. Govan. 2002. Influence of taxonomie status on the in vitro antimicrobial susceptibility of the Burkholderia cepacia complex. J. Antimicrob. Chemother. 50:265-269.
32. Ortega, X. P., S. T. Cardona, A. R. Brown, S. A. Loutet, R. S. Flannagan, D. J. Campopiano, J. R W. Govan, and M. A. Valvano. 2007. A putative gene cluster for aminoarabinose biosynthesis is essential for Burkholderia cenocepacia viability. J. Bacteriol. 189:3639-3644.
33. O'Sullivan, L. A., and E. Mahenthiralingam. 2005. Biotechnological potential within the genus Burkholderia. Lett. Appl. Microbiol. 41:8-11.
34. Parke, J. L, and D. Gurian-Sherman. 2001. Diversity of the Burkholderia cepacia complex and implications for risk assessment of biological control strains. Annu. Rev. Phytopathol. 39:225-258.
35. Poole, K., and R. Srikumar. 2001. Multidrug efflux in Pseudomonas aeruginosa: components, mechanisms and clinical significance. Curr. Top. Med. Chem. 1:59-71.
36. Rajyaguru, J. M., and M. J. Muszynski. 1997. Enhancement of Burkholderia cepacia antimicrobial susceptibility by cationic compounds. J. Antimicrob. Chemother. 40:345-351.
37. 37. Scholz, P., V. Haring, B. Wittmann-Liebold, K. Ashman, M. Bagdasarian, and E. Scherzinger. 1989. Complete nucleotide sequence and gene organization of the broad-host-range plasmid RSF1010. Gene 75:271-288. (Pubitemid 19100128)
38. Schweizer, H. P. 2003. Efflux as a mechanism of resistance to antimicrobials in Pseudomonas aeruginosa and related bacteria: unanswered questions. Genet. Mol. Res. 2:48-62.
39. Tomich, M., C. A. Herfst, J. W. Golden, and C. D. Mohr. 2002. Role of flagella in host cell invasion by Burkholderia cepacia. Infect. Immun. 70: 1799-1806.
40. 40. Tomich, M., and C. D. Mohr. 2004. Genetic characterization of a multicomponent signal transduction system controlling the expression of cable pili in Burkholderia cenocepacia. J. Bacteriol. 186:3826-3836. (Pubitemid 38746058)
41. Vandamme, P., B. Holmes, M. Vancanneyt, T. Coenye, B. Hoste, R Coopman, H. Revets, S. Lauwers, M. Gillis, K. Kersters, and J. R. Govan. 1997. Occurrence of multiple genomovars of Burkholderia cepacia in cystic fibrosis patients and proposal of Burkholderia multivorans sp. nov. Int. J. Syst. Bacteriol. 47:1188-1200.
42. Vanlaere, E., J. J. Lipuma, A. Baldwin, D. Henry, E. De Brandt, E. Mahenthiralingam, D. Speert, C. Dowson, and P. Vandamme. 2008. Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex. Int. J. Syst. Evol. Microbiol. 58:1580-1590.