Striking decrease in the total precursor B-cell compartment during early childhood as evidenced by flow cytometry and gene expression changes

Pediatric Hematology and Oncology

Volumn 27, Issue 1, 2010, Pages 31-45

  Jensen, Kristin ^a   Schaffer, Lana ^b   Olstad, Ole K ^a   Bechensteen, Anne G ^a   Hellebostad, Marit ^a   Tjønnfjord, Geir E ^a   Kierulf, Peter ^a   Gautvik, Kaare M ^a   Osnes, Liv T N ^c  

^a UNIVERSITY OF OSLO   (Norway)

^b SCRIPPS RESEARCH INSTITUTE   (United States)

^c ULLEVÅL UNIVERSITY HOSPITAL   (Norway)

Author keywords

Adults; B cells; Bone marrow; Children; Flow cytometry; Gene expression

Indexed keywords

CD19 ANTIGEN; CD20 ANTIGEN; CD45 ANTIGEN; CD79 ANTIGEN; COMMON ACUTE LYMPHOBLASTIC LEUKEMIA ANTIGEN; RAG1 PROTEIN; TRANSCRIPTION FACTOR PAX5;

ADOLESCENT; ADULT; AGE DISTRIBUTION; ARTICLE; B LYMPHOCYTE DIFFERENTIATION; BONE MARROW EXAMINATION; CELL COMPARTMENTALIZATION; CELL LINEAGE; CONTROLLED STUDY; FLOW CYTOMETRY; GENE EXPRESSION; GENE IDENTIFICATION; HUMAN; INFANT; LYMPHOCYTE COUNT; NORMAL HUMAN; PRE B LYMPHOCYTE; PROTEIN EXPRESSION;

EID: 76649110594     PISSN: 08880018     EISSN: 15210669     Source Type: Journal    
DOI: 10.3109/08880010903420687     Document Type: Article

References (19)

1. Blebea JS, HouseniM, Torigian DA, et al. Structural and functional imaging of normal bonemarrow and evaluation of its age-related changes. Semin Nucl Med. 2007;37:185-194.
2. Suvas S. Advancing age and immune cell dysfunction: is it reversible or not? Expert Opin Biol Ther. 2008;8:657-668.
3. Comans-BitterWM, de Groot R, VanDen BeemdR, et al. Immunophenotyping of blood lymphocytes in childhood: reference values for lymphocyte subpopulations. J Pediatr. 1997;130:388-393.
4. Schatz DG, Oettinger MA, Baltimore D. The V(D)J recombination activating gene, RAG-1. Cell. 1989;59:1035-1048.
5. Oettinger MA, Schatz DG, Gorka C, Baltimore D. RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination. Science. 1990;248(4962):1517-1523.
6. McBlane JF, van Gent DC, Ramsden DA, et al. Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps. Cell. 1995;83:387-395.
7. Langebrake C, Gunther K, Lauber J, Reinhardt D. Preanalytical mRNA stabilization of whole bone marrow samples. Clin Chem. 2007;53:587-593.
8. Bolstad BM, Irizarry RA, Astrand M, Speed TP. A comparison of normalization methods for high density oligonucleotide array data based on variance and bias. Bioinformatics.2003;19:185-194.
9. Irizarry RA, Hobbs B, Collin F, et al. Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics. 2003;4:249-264.
10. Hardin J, Mitani A, Hicks L, VanKoten B. A robust measure of correlation between two genes on a microarray. BMC Bioinformatics. 2007;8:220.
11. van Zelm MC, Van Der Burg M, de Ridder D, et al. Ig gene rearrangement steps are initiated in early human precursor B cell subsets and correlate with specific transcription factor expression. J Immunol. 2005;175:5912-5922.
12. Matthias P, Rolink AG. Transcriptional networks in developing and mature B cells. Nat Rev Immunol. 2005;5:497-508.
13. Boyd RS, Adam PJ, Patel S, et al. Proteomic analysis of the cell-surface membrane in chronic lymphocytic leukemia: identification of two novel proteins, BCNP1 and MIG2B. Leukemia. 2003;17:1605-1612.
14. Sanz C, Benito A, Inohara N, Ekhterae D, NunezG, Fernandez-Luna JL. Specific and rapid induction of the proapoptotic protein Hrk after growth factor withdrawal in hematopoietic progenitor cells. Blood. 2000;95:2742-2747.
15. Yagi T, Takeichi M. Cadherin superfamily genes: functions, genomic organization, and neurologic diversity. Genes Dev. 2000;14:1169-1180. (Pubitemid 30327498)
16. Noordzij JG, de Bruin-Versteeg S, Comans-Bitter WM, et al. Composition of precursor B-cell compartment in bonemarrow from patients with X-linked agammaglobulinemia compared with healthy children. Pediatr Res. 2002;51:159-168.
17. Hystad ME, Bo TH, Rian E, et al. Characterization of Early Human B Cell Development by Gene Expression Profiling. ASH Annual Meeting Abstracts 2006;108:1352.
18. Palmer C, DiehnM, Alizadeh AA, Brown PO. Cell-type specific gene expression profiles of leukocytes in human peripheral blood. BMC Genomics. 2006;7:115.
19. Heng TS, Painter MW. The Immunological Genome Project: networks of gene expression in immune cells. Nat Immunol. 2008;9:1091-1094.