Development of gradient centrifugal partition chromatography method and its application for the isolation of 3,5-dimethoxyphenanthrene-2,7-diol and batatasin-I from Dioscorea opposita

Natural Product Sciences

Volumn 15, Issue 3, 2009, Pages 144-150

  Yoon, Kee Dong ^a   Yang, Min Hye ^a   Chin, Young Won ^a,b   Kim, Yoenjun ^a   Kim, Hye Ryung ^a   Choi, Kiri ^a   Park, Ju Hyun ^c   Kim, Jinwoong ^a  

^a Seoul National University   (South Korea)

^b Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^c Tong Yang Moolsan Co Ltd   (South Korea)

Author keywords

3,5 dimethoxyphenanthrene 2,7 diol; batatasin I; Dioscorea opposita; Gradient centrifugal partition chromatography (GCPC); n hexane methanol water system (HMW, 10 : 9 : 1, v v)

Indexed keywords

3,5 DIMETHOXYPHENANTHRENE 2,7 DIOL; BATATASIN I; DIOSCOREA EXTRACT; HEXANE; METHANOL; PHENANTHRENE DERIVATIVE; PLANT EXTRACT; SILICA GEL; UNCLASSIFIED DRUG; WATER;

ANALYTIC METHOD; ARTICLE; CROP; DIOSCOREA OPPOSITA; DRUG ISOLATION; GRADIENT CENTRIFUGAL PARTITION CHROMATOGRAPHY; PARTITION CHROMATOGRAPHY; VALIDATION PROCESS;

EID: 76649107952     PISSN: 12263907     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. Akahori, A., Studies on the steroidal components of domestic plants-XLIV.: Steroidal sapogenins contained in Japanese Dioscorea sp. Phytochemistry, 4, 97-106 (1965).
2. Bae, K., The Medicinal Plants of Korea, Kyo-Hak, Seoul, p. 551 (1999).
3. Chu, X., Sun, A., and Liu, R., Preparative isolation and purification of five compounds from the Chinese medicinal herb Polygonum cuspidatum Sieb. et Zucc by high-speed counter-current chromatography. J. Chromatogr. A, 1097, 33-39 (2005).
4. Cortez, D.A.G., Marston, A., and Hostettmann, K., Separation of xanthones and a biphenyl from Kielmeyera coriacea centrifugal partition chromatography. Chromatographia, 50, 7- 10 (1999).
5. Coursey, D.E.G., Yam storage-I: A review of yam storage practices and of information on storage losses. J. Stored. Prod. Res., 2, 229-244 (1967).
6. Coxon, D.T., Ogundana, S.K., and Dennis, C., Antifungal phenanthrenes in yam tubers. Phytochemistry, 21, 1389-1392 (1982).
7. Du, Q., Jerz, G., Chen, P., and Winterhalter, P., Preparation of ursane triterpenoids from Centella asiatica using high speed countercurrent chromatography with step-gradient elution. J. Liq. Chromatogr. Relat. Technol., 27, 2201-2215 (2004).
8. Foucault, A.P., Theory of Centrifugal Partition Chromatography: Centrifugal Partition Chromatography, Marcel Dekker, New York (1995).
9. Fu, Y.-C., Ferng, L.-H., and Huang, P.-Y., Quantitative analysis of allantoin and allantoic acid in yam tuber, mucilage, skin and bulbil of the Dioscorea species. Food Chem., 94, 541-549 (2006).
10. Fu, Y.-C., Huang, P.-Y., and Chu, C.-J., Use of continuous bubble separation process for separating and recovering starch and mucilage from yam (Dioscorea pseudojaponica yamamoto). LWT-Food Sci. Technol., 38, 735-744 (2005).
11. Himbert, F., Pennanec, R., Guillaumet, G., and Lafosse, M., Preparative liquid chromatography and centrifugal partition chromatography for purification of new anticancer precursors. Chromatographia, 60, 269-274 (2004).
12. Jayakody, L., Hoover, R., Liu, Q., and Donner, E., Studies on tuber starches II. Molecular structure, composition and physicochemical properties of yam (Dioscorea sp.) starches grown in Sri Lanka. Carbohydr. Polym., 69, 148-163 (2007).
13. Kim, C.Y. and Kim, J., Preparative isolation and purification of geniposide from gardenia fruits by centrifugal partition chromatography. Phytochem. Anal., 18, 115-117 (2007).
14. Leitao, G., El-Adji, S.S., Lopes de Melo, W.A., Leitao, S.G., and Brown, L., Separation of free and glycosylated flavonoids from Siparuna guianensis by gradient and isocratic CCC. J. Liq. Chromatogr. Relat. Technol., 28, 2041- 2051(2005).
15. Leong, Y.-W., Kang, C.-C., Harrison, L.J., and Powell, A.D., Phenanthrenes, dihydrophenanthrenes and bibenzyls from the orchid Bulbophyllum vaginatum. Phytochemistry, 44, 157- 165 (1997).
16. Liu, A., Li, A., and Sun, J., Preparative isolation and purification of coumarins from Angelica dahurica (Fisch. ex Hoffm) Benth, et Hook. f (Chinese traditional medicinal herb) by high-speed counter-current chromatography. J. Chromatogr. A, 1052, 223- 227 (2004a).
17. Liu, R., Lei, F., Sun, A., and Kong, L., Preparative isolation and purification of coumarins from Peucedanum praeruptorum Dunn by high-speed counter-current chromatography. J. Chromatogr. A, 1057, 89-94 (2004b).
18. Marchal, L., Legrand, J., and Foucault, A., Centrifugal partition chromatography: A survey of its history, and our recent advances in the field. Chem. Rec., 3, 133-143 (2003).
19. Murayama, W., Kobayashi, T., Kosuge, Y., Yano, H., Nunogaki, Y., and Nunogaki, K., A new centrifugal counter-current chromatograph and its applications. J. Chromatogr., 239, 643- 649 (1982).
20. Oliveira, R., Leitao, G.G., Moraes, M.C.C., Kaplan, M.A.C., Lopes, D., and Jorge, J.P.P., Gradient elution for triterpene separation from Cecropia lyratiloba Miquel by HSCCC. J. Liq. Chromatogr. Relat. Technol., 28, 1985-1992 (2005).
21. Takasugi, M., Kawashima, S., Monde, K., Katusi, N., Masamune, T., and Shrrata, A., Antifungal compounds from Dioscorea batatas inoculated with Pseudomonas cichorii. Phytochemistry, 26, 371-375 (1987).
22. Yoon, K.D. and Kim, J., Preparative separation of dioscin derivatives from Dioscorea villosa by centrifugal partition chromatography coupled with evaporative light scattering detection. J. Sep. Sci., 31, 2486-2491 (2008).
23. Yoon, K.D., Yang, M.H., Nam, S.I., Park, J.H., Kim, Y.C., and Kim, J., Phenanthrene derivatives, 3,5-dimethoxyphenanthrene-2,7-diol and batatasin-I, as non-polar standard marker compounds for Dioscorea rhizoma. Nat. Prod. Sci., 13, 378-383 (2007).
24. Zhang, L., Liu, Y., and Chen, G., Simultaneous determination of allantoin, choline and -arginine in Rhizoma Dioscoreae by capillary electrophoresis. J. Chromatogr. A, 1043, 317- (2004).
25. Zhao, G., Kan, J., Li, Z., and Chen. Z., Structural features and immunological activity of a polysaccharide from Dioscorea opposita Thunb roots. Carbohydr. Polym., 61, 125-131 (2005).