DNA methyltransferase 3a expression decreases during apoptosis in primary cultures of hepatocytes

Toxicology in Vitro

Volumn 24, Issue 2, 2010, Pages 445-451

  Vinken, Mathieu ^a   Snykers, Sarah ^a   Fraczek, Joanna ^a   Decrock, Elke ^b   Leybaert, Luc ^b   Rogiers, Vera ^a   Vanhaecke, Tamara ^a  

^a FACULTY OF MEDICINE AND PHARMACY   (Belgium)

^b GHENT UNIVERSITY   (Belgium)

Author keywords

Apoptosis; Cell death; DNA methyltransferase; Epigenetics; Primary hepatocyte

Indexed keywords

DNA METHYLTRANSFERASE 3A; FAS ANTIGEN; HOE 33342; LIPOCORTIN 5; MESSENGER RNA; PROPIDIUM IODIDE;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL DEATH; CONTROLLED STUDY; DNA METHYLATION; DOWN REGULATION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME SYNTHESIS; EPIGENETICS; IN VITRO STUDY; LIVER CELL; MALE; MESSENGER RNA SYNTHESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STAINING; TRANSCRIPTION TERMINATION;

ANIMALS; APOPTOSIS; CELLS, CULTURED; CYCLOHEXIMIDE; DNA (CYTOSINE-5-)-METHYLTRANSFERASE; DNA METHYLATION; FAS LIGAND PROTEIN; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HEPATOCYTES; MALE; RATS; RATS, SPRAGUE-DAWLEY;

EID: 76549116110     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tiv.2009.10.003     Document Type: Article

References (35)

1. Bai, L., Wang, J., Yin, X.M., Dong, Z., 2003. Analysis of apoptosis: basic principles and procedures. In: Yin, X.M., Dong, Z. (Eds.), Essentials of Apoptosis: A Guide for Basic and Clinical Research. New Jersey, pp. 239-251.
2. Beaulieu N., Morin S., Chute I.C., Robert M.F., Nguyen H., and MacLeod A.R. An essential role for DNA methyltransferase DNMT3B in cancer cell survival. Journal of Biological Chemistry 277 (2002) 28176-28181
3. Bergmeyer, H.U., 1974. Lactate dehydrogenase. In: Bergmeyer, H.U. (Ed.), Methods of Enzymatic Analysis. New York, pp. 574-579.
4. Boix-Chornet M., Fraga M.F., Villar-Garea A., Caballero R., Espada J., Nunez A., Casado J., Largo C., Casal J.I., Cigudosa J.C., Franco L., Esteller M., and Ballestar E. Release of hypoacetylated and trimethylated histone H4 is an epigenetic marker of early apoptosis. Journal of Biological Chemistry 281 (2006) 13540-13547
5. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72 (1976) 248-254
6. Cheng X., and Blumenthal R.M. Mammalian DNA methyltransferases: a structural perspective. Structure 16 (2008) 341-350
7. Deng T., and Zhang Y. Possible involvement of activation of P53/P21 and demethylation of RUNX 3 in the cytotoxicity against Lovo cells induced by 5-aza-2′-deoxycytidine. Life Sciences 84 (2009) 311-320
8. Elaut G., Henkens T., Papeleu P., Snykers S., Vinken M., Vanhaecke T., and Rogiers V. Molecular mechanisms underlying the dedifferentiation process of isolated hepatocytes and their cultures. Current Drug Metabolism 7 (2006) 629-660
9. Foster, J.R., 2000. Detection and biomarkers of apoptosis. In: Roberts, R. (Ed.), Apoptosis in Toxicology. London, pp. 213-232.
10. Gill, G.H., Dive, D., 2000. Apoptosis: basic mechanisms and relevance to toxicology. In: Roberts, R. (Ed.), Apoptosis in Toxicology. London, pp. 1-20.
11. Gius D., Cui H., Bradbury C.M., Cook J., Smart D.K., Zhao S., Young L., Brandenburg S.A., Hu Y., Bisht K.S., Ho A.S., Mattson D., Sun L., Munson P.J., Chuang E.Y., Mitchell J.B., and Feinberg A.P. Distinct effects on gene expression of chemical and genetic manipulation of the cancer epigenome revealed by a multimodality approach. Cancer Cell 6 (2004) 361-371
12. Gomez-Lechon M.J., O'Connor J.E., Lahoz A., Castell J.V., and Donato M.T. Identification of apoptotic drugs: multiparametric evaluation in cultured hepatocytes. Current Medicinal Chemistry 15 (2008) 2071-2085
13. Gopisetty G., Ramachandran K., and Singal R. DNA methylation and apoptosis. Molecular Immunology 43 (2006) 1729-1740
14. Jackson-Grusby L., Beard C., Possemato R., Tudor M., Fambrough D., Csankovszki G., Dausman J., Lee P., Wilson C., Lander E., and Jaenisch R. Loss of genomic methylation causes p53-dependent apoptosis and epigenetic deregulation. Nature Genetics 27 (2001) 31-39
15. Kassis E.S., Zhao M., Hong J.A., Chen G.A., Nguyen D.M., and Schrump D.S. Depletion of DNA methyltransferase 1 and/or DNA methyltransferase 3b mediates growth arrest and apoptosis in lung and esophageal cancer and malignant pleural mesothelioma cells. Journal of Thoracic and Cardiovascular Surgery 131 (2006) 298-306
16. Lin C.H., Hsieh S.Y., Sheen I.S., Lee W.C., Chen T.C., Shyu W.C., and Liaw Y.F. Genome-wide hypomethylation in hepatocellular carcinogenesis. Cancer Research 61 (2001) 4238-4243
17. Majumder S., Ghoshal K., Datta J., Bai S., Dong X., Quan N., Plass C., and Jacob S.T. Role of de novo DNA methyltransferases and methyl CpG-binding proteins in gene silencing in a rat hepatoma. Journal of Biological Chemistry 277 (2002) 16048-16058
18. Malhi H., and Gores G.J. Cellular and molecular mechanisms of liver injury. Gastroenterology 134 (2008) 1641-1654
19. Malhi H., Gores G.J., and Lemasters J.J. Apoptosis and necrosis in the liver: a tale of two deaths?. Hepatology 43 (2006) S31-S44
20. Nagai M., Nakamura A., Makino R., and Mitamura K. Expression of DNA (5-cytosin)-methyltransferases (DNMTs) in hepatocellular carcinomas. Hepatology Research 26 (2003) 186-191
21. Ni R., Tomita Y., Matsuda K., Ichihara A., Ishimura K., Ogasawara J., and Nagata S. Fas-mediated apoptosis in primary cultured mouse hepatocytes. Experimental Cell Research 215 (1994) 332-337
22. Oh B.K., Kim H., Park H.J., Shim Y.H., Choi J., Park C., and Park Y.N. DNA methyltransferase expression and DNA methylation in human hepatocellular carcinoma and their clinicopathological correlation. International Journal of Molecular Medicine 20 (2007) 65-73
23. Papeleu P., Vanhaecke T., Henkens T., Elaut G., Vinken M., Snykers S., and Rogiers V. Isolation of rat hepatocytes. Methods in Molecular Biology 320 (2006) 229-237
24. Park H.J., Yu E., and Shim Y.H. DNA methyltransferase expression and DNA hypermethylation in human hepatocellular carcinoma. Cancer Letters 233 (2006) 271-278
25. Phipps S.M., Love W.K., Mott T.E., Andrews L.G., and Tollefsbol T.O. Differential expression of epigenetic modulators during human embryonic stem cell differentiation. Molecular Biotechnology 41 (2009) 201-207
26. Qiao L., and Farrell G.C. The effects of cell density, attachment substratum and dexamethasone on spontaneous apoptosis of rat hepatocytes in primary culture. In Vitro Cellular and Developmental Biology Animal 35 (1999) 417-424
27. Robertson K.D., Uzvolgyi E., Liang G., Talmadge C., Sumegi J., Gonzales F.A., and Jones P.A. The human DNA methyltransferases (DNMTs) 1, 3a and 3b: coordinate mRNA expression in normal tissues and overexpression in tumors. Nucleic Acids Research 27 (1999) 2291-2298
28. Rouquet N., Carlier K., Briand P., Wiels J., and Joulin V. Multiple pathways of Fas-induced apoptosis in primary culture of hepatocytes. Biochemical and Biophysical Research Communications 229 (1996) 27-35
29. Saito Y., Kanai Y., Nakagawa T., Sakamoto M., Saito H., Ishii H., and Hirohashi S. Increased protein expression of DNA methyltransferase (DNMT) 1 is significantly correlated with the malignant potential and poor prognosis of human hepatocellular carcinomas. International Journal of Cancer 105 (2003) 527-532
30. Schneider-Stock R., Diab-Assef M., Rohrbeck A., Foltzer-Jourdainne C., Boltze C., Hartig R., Schonfeld P., Roessner A., and Gali-Muhtasib H. 5-Aza-cytidine is a potent inhibitor of DNA methyltransferase 3a and induces apoptosis in HCT-116 colon cancer cells via Gadd45- and p53-dependent mechanisms. Journal of Pharmacology and Experimental Therapeutics 312 (2005) 525-536
31. Szyf M. The dynamic epigenome and its implications in toxicology. Toxicological Sciences 100 (2007) 7-23
32. Takashima S., Takehashi M., Lee J., Chuma S., Okano M., Hata K., Suetake I., Nakatsuji N., Miyoshi H., Tajima S., Tanaka Y., Toyokuni S., Sasaki H., Kanatsu-Shinohara M., and Shinohara T. Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects. Biology of Reproduction 81 (2009) 155-164
33. Turek-Plewa J., and Jagodzinski P.P. The role of mammalian DNA methyltransferases in the regulation of gene expression. Cellular and Molecular Biology Letters 10 (2005) 631-647
34. Vinken M., Papeleu P., Snykers S., De Rop E., Henkens T., Chipman J.K., Rogiers V., and Vanhaecke T. Involvement of cell junctions in hepatocyte culture functionality. Critical Reviews in Toxicology 36 (2006) 299-318
35. Vinken M., Henkens T., Snykers S., Lukaszuk A., Tourwé D., Rogiers V., and Vanhaecke T. The novel histone deacetylase inhibitor 4-Me2N-BAVAH differentially affects cell junctions between primary hepatocytes. Toxicology 236 (2007) 92-102