Monooxygenation of rifampicin catalyzed by the rox gene product of Nocardia farcinica: Structure elucidation, gene identification and role in drug resistance

Journal of Antibiotics

Volumn 63, Issue 1, 2010, Pages 23-28

  Hoshino, Yasutaka ^a   Fujii, Shoko ^a,b   Shinonaga, Hideki ^c   Arai, Koshi ^c   Saito, Fumiko ^a   Fukai, Toshio ^d   Satoh, Hiroyuki ^b   Miyazaki, Yoshitsugu ^a   Ishikawa, Jun ^a  

^a NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

^b TOHO UNIVERSITY   (Japan)

^c TAISHO PHARMACEUTICAL CO LTD   (Japan)

^d YOKOHAMA COLLEGE OF PHARMACY   (Japan)

Author keywords

Drug resistance; Monooxygenase; Nocardia farcinica; Rifampicin; Rox

Indexed keywords

GENE PRODUCT; RIFAMPICIN; RIFAMPICIN MONOOXYGENASE; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; CATALYSIS; DELETION MUTANT; GENE DELETION; GENE MUTATION; GENE OVEREXPRESSION; NOCARDIA FARCINICA; NONHUMAN; NUCLEOTIDE SEQUENCE; OXYGENATION; PRIORITY JOURNAL; ROX GENE; WILD TYPE;

ANTI-BACTERIAL AGENTS; BACTERIA; BACTERIAL PROTEINS; BIOTRANSFORMATION; DRUG RESISTANCE, BACTERIAL; GENE DELETION; GENE DOSAGE; GENE ORDER; MAGNETIC RESONANCE SPECTROSCOPY; MICROBIAL SENSITIVITY TESTS; MIXED FUNCTION OXYGENASES; MOLECULAR STRUCTURE; NOCARDIA; PLASMIDS; RIFAMPIN;

NOCARDIA FARCINICA;

EID: 76449113588     PISSN: 00218820     EISSN: None     Source Type: Journal    
DOI: 10.1038/ja.2009.116     Document Type: Article

References (14)

1. Ishikawa,J. et al. The complete genomic sequence of Nocardia farcinica IFM 10152. Proc. Natl Acad. Sci. USA 101, 14925-14930 (2004).
2. Ishikawa, J., Chiba, K., Kurita, H. & Satoh, H. Contribution of rpoB2 RNA polymerase b subunit gene to rifampin resistance in Nocardia species. Antimicrob. Agents Chemother. 50, 1342-1346 (2006).
3. Tanaka, Y. et al. Different rifampicin inactivation mechanisms in Nocardia and related taxa. Microbiol. Immunol. 40, 1-4 (1996).
4. Yazawa, K. et al. Inactivation of rifampin by Nocardia brasiliensis. Antimicrob. Agents Chemother. 37, 1313-1317 (1993).
5. Yazawa, K., Mikami, Y., Maeda, A., Morisaki, N. & Iwasaki, S. Phosphorylative inactivation of rifampicin by Nocardia otitidiscaviarum. J. Antimicrob. Chemother. 33, 1127-1135 (1994).
6. Quan, S., Venter, H. & Dabbs, E. R. Ribosylative inactivation of rifampin by Mycobacterium smegmatis is a principal contributor to its low susceptibility to this antibiotic. Antimicrob. Agents Chemother. 41, 2456-2460 (1997).
7. Andersen, S. J., Quan, S., Gowan, B. & Dabbs, E. R. Monooxygenase-like sequence of a Rhodococcus equi gene conferring increased resistance to rifampin by inactivating this antibiotic. Antimicrob. Agents Chemother. 41, 218-221 (1997).
8. Chiba, K. et al. Construction of a pair of practical Nocardia-Escherichia coli shuttle vectors. Jpn. J. Infect. Dis. 60, 45-47 (2007).
9. Schäfer, A. et al. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145, 69-73 (1994).
10. Ishikawa, J., Tsuchizaki, N., Yoshida, M., Ishiyama, D. & Hotta, K. Colony PCR for detection of specific DNA sequences in Actinomycetes. Actinomycetologica 14, 1-5 (2000).
11. Tamura, K., Dudley, J., Nei, M. & Kumar, S. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24, 1596-1599 (2007).
12. Casey, M. L. & Whitlock, H. W. Determination of the solution conformation of rifamycin S and derivatives by nuclear magnetic resonance. J. Am. Chem. Soc. 97, 6231-6236 (1975).
13. Walash, M. I., Belal, F., Metwally, M. E. & Hefnawy, M. M. Spectrophotometric determination of rifampin in the presence of its degradation products in pharmaceutical preparations. Anal. Lett. 26, 1905-1917 (1993).
14. Ripamonti, A., Ferrari, P. & Gallo, G. G. Structure identification of rifampicin N-oxide. Farmaco. Sci. 39, 806-809 (1984).