Evaluation of an antibody-free ADP fetection assay: ADP-Glo

Assay and Drug Development Technologies

Volumn 7, Issue 6, 2009, Pages 598-605

  Li, Hu ^a   Totoritis, Rachel D ^a   Lor, Leng A ^a   Schwartz, Benjamin ^a   Caprioli, Peter ^a   Jurewicz, Anthony J ^a   Zhang, Guofeng ^a  

^a GLAXOSMITHKLINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; CALCIUM CALMODULIN DEPENDENT PROTEIN KINASE II; LUCIFERASE; LUCIFERIN; PROTEIN KINASE; PROTEIN KINASE INHIBITOR; PROTEIN TYROSINE KINASE; REAGENT;

ACCURACY; ARTICLE; ASSAY; AUTOPHOSPHORYLATION; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; DRUG TARGETING; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME INHIBITION; ENZYME MECHANISM; EVALUATION; FLUORESCENCE POLARIZATION; HIGH THROUGHPUT SCREENING; SCINTILLATION PROXIMITY ASSAY; SENSITIVITY ANALYSIS; TECHNOLOGY;

ADENOSINE DIPHOSPHATE; ANTIBODIES; CHEMISTRY TECHNIQUES, ANALYTICAL; IMMUNOASSAY; LUMINESCENT MEASUREMENTS; LUMINESCENT PROTEINS; PHOSPHOTRANSFERASES; PROTEIN INTERACTION MAPPING; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 76449107446     PISSN: 1540658X     EISSN: None     Source Type: Journal    
DOI: 10.1089/adt.2009.0221     Document Type: Article

References (20)

1. Cohen P: Protein kinases-the major drug targets of the twenty-first century? Nat Rev Drug Discov 2002;1:309-315.
2. Garber K: The second wave in kinase cancer drugs. Nat Biotechnol 2006;24: 127-130.
3. Druker BJ: Current treatment approaches for chronic myelogenous leukemia. Cancer J 2001;7(Suppl 1):S14-S18.
4. Druker BJ, Talpaz M, Resta DJ, Peng B, Buchdunger E, Ford JM, et al.: Efficacy and safety of a specific inhibitor of the BCR-ABL tyrosine kinase in chronic myeloid leukemia. N Engl J Med 2001;344:1031-1037.
5. Eglen RM, Reisine T: The current status of drug discovery against the human kinome. Assay Drug Dev Technol 2009;7:22-43.
6. Cohen P: Targeting protein kinases for the development of anti-inflammatory drugs. Curr Opin Cell Biol 2009;21:317-324.
7. Gaestel M, Kotlyarov A, Kracht M: Targeting innate immunity protein kinase signalling in inflammation. Nat Rev Drug Discov 2009;8:480-499.
8. Singh P, Harden BJ, Lillywhite BJ, Broad PM: Identification of kinase inhibitors by an ATP depletion method. Assay Drug Dev Technol 2004;2:161-169.
9. Munagala N, Nguyen S, Lam W, Lee J, Joly A, McMillan K, et al.: Identification of small molecule ceramide kinase inhibitors using a homogeneous chemiluminescence high throughput assay. Assay Drug Dev Technol 2007;5:65-73.
10. Koresawa M, Okabe T: High-throughput screening with quantitation of ATP consumption: a universal non-radioisotope, homogeneous assay for protein kinase. Assay Drug Dev Technol 2004;2:153-160.
11. Lowery RG, Kleman-Leyer K: Transcreener: screening enzymes involved in covalent regulation. Expert Opin Ther Targets 2006;10:179-190.
12. Charter NW, Kauffman L, Singh R, Eglen RM: A generic, homogenous method for measuring kinase and inhibitor activity via adenosine 5?-diphosphate accumulation. J Biomol Screen 2006;11:390-399.
13. Jia Y, Quinn CM, Kwak S, Talanian RV: Current in vitro kinase assay technologies: the quest for a universal format. Curr Drug Discov Technol 2008;5:59-69.
14. Zhang JH, Chung TD, Oldenburg KR: A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays. J Biomol Screen 1999;4:67-73.
15. Macarrón R, Hertzberg RP: Design and implementation of high throughput screening assays. Methods Mol Biol 2002;190:1-29.
16. Yang J, Copeland RA, Lai Z: Defi ning balanced conditions for inhibitor screening assays that target bisubstrate enzymes. J Biomol Screen 2009;14:111-120.
17. Inglese J, Johnson RL, Simeonov A, Xia M, Zheng W, Austin CP, et al.: Highthroughput screening assays for the Identification of chemical probes. Nat Chem Biol 2007;3:466-479.
18. Ma H, Deacon S, Horiuchi K: The challenge of selecting protein kinase assays for lead discovery optimization. Expert Opin Drug Discov 2008;3:607-621.
19. Hong L, Quinn CM, Jia Y: Evaluating the utility of the HTRF Transcreener ADP assay technology: a comparison with the standard HTRF assay technology. Anal Biochem 2009;391:31-38.
20. Jia Y, Gu X, Brinker A, Warmuth M: Measuring the tyrosine kinase activity: a review of biochemical and cellular assay technologies. Expert Opin Drug Discov 2008;3:959-978.