Anti-proliferative efficacy of icariin on HepG2 hepatoma and its possible mechanism of action

American Journal of Chinese Medicine

Volumn 37, Issue 6, 2009, Pages 1153-1165

  Yang, Jin Xia ^a   Fichtner, Iduna ^b   Becker, Monika ^b   Lemm, Margit ^b   Wang, Xue Mei ^a  

^a PEKING UNIVERSITY   (China)

^b MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

Author keywords

CD31; Flow Cytometry; HepG2; Icariin; Immunohistochemical Assay; Ki67; MTT Assay

Indexed keywords

CD31 ANTIGEN; ICARIIN; KI 67 ANTIGEN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIANGIOGENIC ACTIVITY; ARTICLE; BODY WEIGHT; BONE MARROW; CANCER INHIBITION; CD4 CD8 RATIO; CD4+ T LYMPHOCYTE; CD8+ T LYMPHOCYTE; CELL STRAIN HEPG2; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG EFFICACY; DRUG MECHANISM; DRUG SCREENING; DRUG STRUCTURE; FLOW CYTOMETRY; HEMATOLOGICAL PARAMETERS; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; IN VIVO STUDY; LIVER CELL CARCINOMA; MALE; MOUSE; NONHUMAN; PROTEIN EXPRESSION; SPLEEN WEIGHT; TUMOR GROWTH; TUMOR VOLUME;

ANIMALS; ANTIGENS, CD19; ANTIGENS, CD31; ANTINEOPLASTIC AGENTS, PHYTOGENIC; BONE MARROW; CARCINOMA, HEPATOCELLULAR; CD4-CD8 RATIO; CD4-POSITIVE T-LYMPHOCYTES; CD8-POSITIVE T-LYMPHOCYTES; CELL LINE, TUMOR; CELL PROLIFERATION; DOSE-RESPONSE RELATIONSHIP, DRUG; EPIMEDIUM; FLAVONOIDS; FLOW CYTOMETRY; HEP G2 CELLS; KI-67 ANTIGEN; LIVER NEOPLASMS, EXPERIMENTAL; MALE; MICE; MICE, NUDE; PHYTOTHERAPY; PLANT EXTRACTS; XENOGRAFT MODEL ANTITUMOR ASSAYS;

EID: 76449085813     PISSN: 0192415X     EISSN: None     Source Type: Journal    
DOI: 10.1142/S0192415X09007569     Document Type: Article

References (27)

1. Adnan, Z., C. Margaret and H. Brian. Value of measuring subpopulations of T and B lymphocytes in patients with musculoskeletal tumours. Arch. Orthop. Trauma Surg. 128: 921-929, 2008.
2. Albelda, S.M., W.A. Muller, C.A. Buck and P.J. Newman. Molecular and cellular properties of PECAM-1 (endoCAM/CD31): a novel vascular cell-cell adhesion molecule. J. Cell Biol. 114(5): 1059-1068, 1991.
3. Appay, V., D.C. Douek and D.A. Price. CD8+ T cell efficacy in vaccination and disease. Nat. Med. 14(6): 623-628, 2008.
4. Attoub, S., A.M. Gaben, S. Al-Salam, M.A. Al-Sultan, A. John, M.G. Nicholls, J. Mester and G. Petroianu. Captopril as a potential inhibitor of lung tumor growth and metastasis. Ann. N.Y. Acad. Sci. 1138: 65-72, 2008.
5. Baluk, P., J. Fuxe,H.Hashizume, T.Romano, E. Lashnits, S.Butz,D.Vestweber, M.Corada,C.Molendini, E. Dejana and D.M. McDonald. Functionally specialized junctions between endothelial cells of lymphatic vessels. J. Exp. Med. 204(10): 2349-2362, 2007.
6. Chen, K.M., B.F. Ge, X.Y. Liu, P.H. Ma, M.B. Lu, M.H. Bai and Y. Wang. Icariin inhibits the osteoclast formation induced by RANKL and macrophage-colony stimulating factor in mouse bone marrow culture. Pharmazie 62(5): 388-391, 2007.
7. Dobrzanski, M.J., J.B. Reome and R.W. Dutton. Therapeutic effects of tumor-reactive type 1 and type 2 CD8+ T cell subpopulations in established pulmonary metastases. J. Immunol. 162: 6671-6680, 1999.
8. El-Serag, H.B. and K.L. Rudolph. Hepatocellular carcinoma: epidemiology and molecular carcinogenesis. Gastroenterology 132: 2557-2576, 2007.
9. Folkman, J. What is the evidence that tumors are angiogenesis dependent? J. Natl. Cancer Inst. 82: 4-6, 1990.
10. Fujimoto, M. and S. Sato. B cell signaling and autoimmune diseases: CD19/CD22 loop as a B cell signaling device to regulate the balance of autoimmunity. J. Dermatol. Sci. 46(1): 1-9, 2007.
11. Huang, J., L.Yuan, X.Wang, T.L. Zhang and K.Wang. Icaritin and its glycosides enhance osteoblastic, but suppress osteoclastic, differentiation and activity in vitro. Life Sci. 81(10): 832-840, 2007a.
12. Huang, X., D. Zhu and Y. Lou. A novel anticancer agent, icaritin, induced cell growth inhibition, G1 arrest and mitochondrial transmembrane potential drop in human prostate carcinoma PC-3 cells. Eur. J. Pharmacol. 14 (1-3): 26-36, 2007b.
13. Ji, R.R., F.Y. Li and X.J. Zhang. Effect of icariin on hypoxia induced vascular endothelial cells injury. Zhongguo Zhong Xi Yi Jie He Za Zhi 25(6): 525-530, 2005.
14. Lau, W.Y. and E.C. Lai. Hepatocellular carcinoma: current management and recent advances. Hepatobiliary Pancreat. Dis. Int. 7(3): 237-257. 2008.
15. Li, L., Q.X. Zhou and J.S. Shi. Protective effects of icariin on neurons injured by cerebral ischemia/reperfusion. Chin. Med. J. (Engl.) 5(19): 1637-1643, 2005.
16. Liu,W.J., Z.C.Xin,H.Xin, Y.M.Yuan, L. Tian and Y.L.Guo. Effects of icariin on erectile function and expression of nitric oxide synthase isoforms in castrated rats. Asian J. Androl. 7(4): 381-388, 2005.
17. Masuzaki, R. and M. Omata. Treatment of hepatocellular carcinoma. Indian J. Gastroenterol. 27(3): 113-122, 2008.
18. Nico,B.,V. Benagiano, D.Mangieri, N.Maruotti, A.Vacca andD.Ribatti. Evaluation ofmicrovascular density in tumors: pro and contra. Histol. Histopathol. 23(5): 601-607, 2008.
19. Pan, Y., L.D. Kong, Y.C. Li, X. Xia, H.F. Kung and F.X. Jiang. Icariin from Epimedium brevicornum attenuates chronicmild stress-induced behavioral and neuroendocrinological alterations in male Wistar rats. Pharmacol. Biochem. Behav. 87(1): 130-140, 2007.
20. Scholzen, T. and J. Gerdes. The Ki-67 protein: from the known and the unknown. J. Cell. Physiol. 182(3): 311-322, 2000.
21. Wang, T., J.C. Zhang, Y. Chen, F. Huang, M.S. Yang and P.G. Xiao. Comparison of antioxidative and antitumor activities of six flavonoids from Epimedium koreanum. Zhongguo Zhong Yao Za Zhi 32(8): 715-718, 2007.
22. Wicks, S.M., R. Tong, C.Z. Wang, M. O'Connor, T. Karrison, S. Li, J. Moss and C.S. Yuan. Safety and tolerability of Ganoderma lucidum in healthy subjects: a double-blind randomized placebocontrolled trial. Am. J. Chin. Med. 35: 407-414, 2007.
23. Xu, H.B. and Z.Q. Huang. Icariin enhances endothelial nitric-oxide synthase expression on human endothelial cells in vitro. Vascul. Pharmacol. 47(1): 18-24. 2007.
24. Zhang,D.W.,Y.Cheng,N.L.Wang, J.C. Zhang, M.S.Yang andX.S.Yao. Effects of total flavonoids and flavonol glycosides from Epimedium koreanum Nakai on the proliferation and differentiation of primary osteoblasts. Phytomedicine 15(1-2): 55-61, 2008. (Pubitemid 350297899)
25. Zhang, Z.B. and Q.T. Yang. The testosterone mimetic properties of icariin. Asian J. Androl. 8(5): 601-605, 2006.
26. Zhao, F.,Y.Z. Tang and Z.Q. Liu. Protective effect of icariin on DNA against radical-induced oxidative damage. J. Pharm. Pharmacol. 59(12): 1729-1732, 2007.
27. Ziche, M. and P.M. Gullino. Angiogenesis and neoplastic progression in vitro. J. Natl. Cancer Inst. 69: 483-487, 1982.