NCLX is an essential component of mitochondrial Na+/Ca 2+ exchange

Proceedings of the National Academy of Sciences of the United States of America

Volumn 107, Issue 1, 2010, Pages 436-441

  Palty, Raz ^a   Silverman, William F ^a   Hershfinkel, Michal ^a   Caporale, Teresa ^a,b   Sensi, Stefano L ^a,b,c   Parnis, Julia ^d   Nolte, Christiane ^d   Fishman, Daniel ^a   Shoshan Barmatz, Varda ^e   Herrmann, Sharon ^a   Khananshvili, Daniel ^e   Sekler, Israel ^a  

^a BEN GURION UNIVERSITY OF THE NEGEV   (Israel)

^b UNIVERSITY OF CHIETI   (Italy)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

^e TEL AVIV UNIVERSITY   (Israel)

Author keywords

CGP 37157; Mitochondrial calcium exchanger; Mitochondrial calcium homeostasis; Sodium calcium exchanger

Indexed keywords

7 CHLORO 5 (2 CHLOROPHENYL) 1,5 DIHYDRO 4,1 BENZOTHIAZEPIN 2(3H) ONE; LITHIUMSS; NCLX PROTEIN; SMALL INTERFERING RNA; SODIUM CALCIUM EXCHANGE PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; EMBRYO; FLUORESCENCE IMAGING; GENE SILENCING; HUMAN; HUMAN CELL; MITOCHONDRION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; SODIUM CALCIUM EXCHANGE;

ANIMALS; BRAIN; CALCIUM; CLONAZEPAM; HOMEOSTASIS; HUMANS; MICE; MITOCHONDRIA; MITOCHONDRIAL MEMBRANES; MYOCARDIUM; RATS; SODIUM; SODIUM-CALCIUM EXCHANGER; THIAZEPINES;

EID: 76249133414     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0908099107     Document Type: Article

References (37)

1. Szabadkai G, Duchen MR (2008) Mitochondria: the hub of cellular Ca2+ signaling. Physiology (Bethesda) 23:84-94.
2. Jiang D, Zhao L, Clapham DE (2009) Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter. Science 326:144-147.
3. Gunter TE, Buntinas L, Sparagna G, Eliseev R, Gunter K (2000) Mitochondrial calcium transport: mechanisms and functions. Cell Calcium 28:285-296.
4. Cox DA, Matlib MA (1993) A role for the mitochondrial Na(+)-Ca2+ exchanger in the regulation of oxidative phosphorylation in isolated heart mitochondria. J Biol Chem 268:938-947.
5. Szabadkai G, et al. (2006) Mitochondrial dynamics and Ca2+ signaling. Biochim Biophys Acta 1763:442-449.
6. Carafoli E, Tiozzo R, Lugli G, Crovetti F, Kratzing C (1974) The release of calcium from heart mitochondria by sodium. J Mol Cell Cardiol 6:361-371.
7. Palty R, et al. (2004) Lithium-calcium exchange is mediated by a distinct potassium-independent sodium-calcium exchanger. J Biol Chem 279:25234-25240.
8. Lytton J (2007) Na+/Ca2+ exchangers: three mammalian gene families control Ca2+ transport. Biochem J 406:365-382.
9. Cai X, Lytton J (2004) Molecular cloning of a sixth member of the K+-dependent Na+/Ca2+ exchanger gene family, NCKX6. J Biol Chem 279:5867-5876.
10. Paucek P, Jabůrek M (2004) Kinetics and ion specificity of Na(+)/Ca(2+) exchange mediated by the reconstituted beef heart mitochondrial Na(+)/Ca(2+) antiporter. Biochim Biophys Acta 1659:83-91.
11. Li W, et al. (1992) Reconstitution, identification, purification, and immunological characterization of the 110-kDa Na+/Ca2+ antiporter from beef heart mitochondria. J Biol Chem 267:17983-17989.
12. Nagai T, Sawano A, Park ES, Miyawaki A (2001) Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc Natl Acad Sci USA 98:3197-3202.
13. Malli R, et al. (2003) Sustained Ca2+ transfer across mitochondria is Essential for mitochondrial Ca2+ buffering, sore-operated Ca2+ entry, and Ca2+ store refilling. J Biol Chem 278:44769-44779.
14. Palty R, et al. (2006) Single alpha-domain constructs of the Na+/Ca2+ exchanger, NCLX, oligomerize to form a functional exchanger. Biochemistry 45:11856-11866.
15. Cox DA, Conforti L, Sperelakis N, Matlib MA (1993) Selectivity of inhibition of Na(+)-Ca2+ exchange of heart mitochondria by benzothiazepine CGP-37157. J Cardiovasc Pharmacol 21:595-599.
16. Luciani DS, Ao P, Hu X, Warnock GL, Johnson JD (2007) Voltage-gated Ca(2+) influx and insulin secretion in human and mouse beta-cells are impaired by the mitochondrial Na(+)/Ca(2+) exchange inhibitor CGP-37157. Eur J Pharmacol 576:18-25.
17. 3H)-one (CGP-37157) in cerebellar granule cells. Biochem Pharmacol 66:2409-2411.
18. 1H-4,1-benzothiazepine-2-one (CGP-37157). J Pharmacol Exp Ther 306:1050-1057.
19. Crompton M, Künzi M, Carafoli E (1977) The calcium-induced and sodium-induced effluxes of calcium from heart mitochondria. Evidence for a sodium-calcium carrier. Eur J Biochem 79:549-558.
20. Cai X, Lytton J (2004) The cation/Ca(2+) exchanger superfamily: phylogenetic analysis and structural implications. Mol Biol Evol 21:1692-1703.
21. Kar P, Chakraborti T, Samanta K, Chakraborti S (2008) mu-Calpain mediated cleavage of the Na+/Ca2+ exchanger in isolated mitochondria under A23187 induced Ca2+ stimulation. Arch Biochem Biophys .
22. Nicholls DG, Chalmers S (2004) The integration of mitochondrial calcium transport and storage. J Bioenerg Biomembr 36:277-281.
23. Hilgemann DW, Yaradanakul A, Wang Y, Fuster D (2006) Molecular control of cardiac sodium homeostasis in health and disease. J Cardiovasc Electrophysiol 17 (Suppl 1): S47-S56.
24. Ginger RS, et al. (2008) SLC24A5 encodes a trans-Golgi network protein with potassium-dependent sodium-calcium exchange activity that regulates human epidermal melanogenesis. J Biol Chem 283:5486-5495.
25. Schultz BD, Frizzell RA, Bridges RJ (1999) Rescue of dysfunctional deltaF508-CFTR chloride channel activity by IBMX. J Membr Biol 170:51-66.
26. Xie X, Wu G, Lu ZH, Rohowsky-Kochan C, Ledeen RW (2004) Presence of sodium-calcium exchanger/GM1 complex in the nuclear envelope of non-neural cells: nature of exchanger-GM1 interaction. Neurochem Res 29:2135-2146.
27. Addya S, et al. (1997) Targeting of NH2-terminal-processed microsomal protein to mitochondria: a novel pathway for the biogenesis of hepatic mitochondrial P450MT2. J Cell Biol 139:589-599.
28. Robin MA, et al. (2000) Vesicular transport of newly synthesized cytochromes P4501A to the outside of rat hepatocyte plasma membranes. J Pharmacol Exp Ther 294:1063-1069.
29. De Marchi U, et al. (2009) Intermediate conductance Ca2+-activated potassium channel (KCa3.1) in the inner mitochondrial membrane of human colon cancer cells. Cell Calcium 45:509-516.
30. Rudolf R, Mongillo M, Magalhães PJ, Pozzan T (2004) In vivo monitoring of Ca(2+) uptake into mitochondria of mouse skeletal muscle during contraction. J Cell Biol 166:527-536.
31. Balemba OB, Bartoo AC, Nelson MT, Mawe GM (2008) Role of mitochondria in spontaneous rhythmic activity and intracellular calcium waves in the guinea pig gallbladder smooth muscle. Am J Physiol Gastrointest Liver Physiol 294:G467-G476.
32. Poburko D, Liao CH, van Breemen C, Demaurex N (2009) Mitochondrial regulation of sarcoplasmic reticulum Ca2+ content in vascular smooth muscle cells. Circ Res 104:104-112.
33. Tinel H, et al. (1999) Active mitochondria surrounding the pancreatic acinar granule region prevent spreading of inositol trisphosphate-evoked local cytosolic Ca(2+) signals. EMBO J 18:4999-5008.
34. Gandhi S, et al. (2009) PINK1-associated Parkinson's disease is caused by neuronal vulnerability to calcium-induced cell death. Mol Cell 33:627-638.
35. Filippin L, Magalhães PJ, Di Benedetto G, Colella M, Pozzan T (2003) Stable interactions between mitochondria and endoplasmic reticulum allow rapid accumulation of calcium in a subpopulation of mitochondria. J Biol Chem 278:39224-39234.
36. Frieden M, et al. (2004) Ca(2+) homeostasis during mitochondrial fragmentation and perinuclear clustering induced by hFis1. J Biol Chem 279:22704-22714.
37. Lee B, et al. (2003) Inhibition of mitochondrial Na+-Ca2+ exchanger increases mitochondrial metabolism and potentiates glucose-stimulated insulin secretion in rat pancreatic islets. Diabetes 52:965-973.