In vitro investigations of Cynara scolymus L. extract on cell physiology of HepG2 liver cells

Brazilian Journal of Pharmaceutical Sciences

Volumn 45, Issue 2, 2009, Pages 201-208

  Löhr, Gesine ^a   Deters, Alexandra ^a   Hensel, Andreas ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

Artichoke; Asteraceae; Cell proliferation; Cynara scolymus L.; Gene expression; Liver cells

Indexed keywords

ALCOHOL; CHLOROGENIC ACID; CYNARA SCOLYMUS EXTRACT; CYNARINE; CYTOCHROME P450 1A2; CYTOCHROME P450 3A4; DIMETHYL SULFOXIDE; FLAVONOID; GLUTATHIONE PEROXIDASE 2; GLUTATHIONE TRANSFERASE; HYDROGEN PEROXIDE; LACTATE DEHYDROGENASE; LIVER ENZYME; LUTEOLIN; LUTEOLIN 7 GLUCOSIDE; QUINIC ACID;

ARTICHOKE; ARTICLE; CELL FUNCTION; CELL PROLIFERATION; CELL PROTECTION; CONTROLLED STUDY; CYTOLOGY; CYTOTOXICITY; DRUG INHIBITION; ENZYME ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION REGULATION; GENE INDUCTION; HUMAN; HUMAN CELL; IN VITRO STUDY; INCUBATION TIME; LIVER CELL; MITOCHONDRION; PROTEIN CONTENT; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EID: 76149099304     PISSN: 19848250     EISSN: 21759790     Source Type: Journal    
DOI: 10.1590/S1984-82502009000200003     Document Type: Article

References (23)

1. 4 toxicity in isolated rat hepatocytes. J. Nat. Prod., v.50, p.612-617, 1987.
2. BRAND, N.; Cynara. In: HÄNSEL, R.; SÉLLER, K., RIMPLER, H., SCHNEIDER, G., (Eds.). Hagers Handbuch der Pharmazeutischen Praxis, Drogen. 5. ed. Berlin, Heidelberg, New York: Springer Publishers, 1992. v.4, p.1117-1131.
3. CANTERO, G.; CAMPANELLA, C.; MATEOS, S.; CORTES, F. Topoisomerase II inhibition and high yield of endoreduplication induced by the flavonoids luteolin and quercetin. Mutagenesis, v.21, p.321-325, 2006.
4. DAUER, A.; HENSEL, A.; LHOSTE, F.; KNASMUELLER, S.; MERSCH-SUNDERMANN, V. Genotoxic and antigenotoxic effects of catechin and tannins from the bark of Hamamelis virginiana L. in metabolically competent, human hepatoma cells (HepG2) using single cell electrophoresis. Phytochem., v.63, p.199-207, 2003.
5. ESCOP Monograph - The Scientific Foundation for Herbal Medicinal Products. Cynarae folium - Artichoke leaf. 2.ed. New York: Thieme Publishers, 2003.
6. GEBHARD, R. Anticholestatic activity of flavonoids from artichoke (Cynara scolymus L.) and their metabolites. Med. Sci. Mon., v.7, supl.1, p.316-320, 2001.
7. GEBHARD, R. Antioxidative and protective properties of extracts from leaves of the artichoke (Cynara scolymus L.) against hydroperoxide- induced oxidative stress in cultured rat hepatocytes. Tox. Apl. Pharmacol., v.144, p.279-286, 1997.
8. GEBHARD, R. Artischockenextrakt - in vitro Nachweis einer Hemmwirkung auf die Cholesterolbiosynthese. Medizin Welt, v.46, p.348-350, 1995.
9. GEBHARD, R. Neue Erkenntnisse zur Wirkung von Artischockenextrakte. Z. Allgemeinmed., v.72, p.20-23, 1996.
10. HOFMANN, T.; DETERS, A.; MÜLLER, G.; STARK, T.; WITTSCHIER, N.; HENSEL, A. Occurence of N-Phenylpropenoyl-L-amino acids in different herbal drugs and influence on human keratinocytes, human liver cells and against adhesion of H. pylori to human stomach. Planta Med., v.73, p.142-150, 2007.
11. LOWRY, O.H.; NIRA, J.; ROSEBROUGH, N.J.; FARR, A.L.; RANDALL, R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. v.193, p.265-275, 1951.
12. MAROS, T.; SERES-STRUMR, L.; RAIZ, G.; RETEJI, C.; KOVACS, V.V.; HINTS, M. Wirkung der Cynara scolymus-Extrakte auf die Regeneration der Rattenleber Drug Res., v.18, p.884-886, 1968.
13. MARTIN, A.; CLYNES, M. Comparison of 5 microplate colorimetric assays for in vitro cytotoxicity testing and cell proliferation assays. Cytotechnology, v.11, p.9-58, 1993.
14. MATUSCHOWSKI, P.; GUMBINGER, H.G.; NAHRSTEDT, A.; WINTERHOFF, H. Testing of Cynara scolymus L. in the isolated perfunded rat liver. Planta Med., v. 63, p. 55-56, 1997.,
15. MATUSCHOWSKI, P.; NAHRSTEDT, A.; WINTERHOFF, H. Pharmakologische Untersuchungen eines Frischpflanzenpresssaftes aus Cynara scolymus auf choleretische Wirkung. Z. Phytother., v.26, p.216-221, 2005.
16. MOSMANN, M. Rapid colorimetric assay for cellular growth and survival: applications to Proliferation and cytotoxicity assays. J. Immunol. Methods, v.65, p.55-63, 1983.
17. PITTLER, M.H.; THOMPSON, C.J.; ERNST, E. Artichoke leaf extract for treating hypercholesterolaemia (Review). The Cochrane Database of Syst. Rev., volume?, Issue 3, pages?, 2002.
18. PORSTMANN, T.; TERNYK, T.; AVRAMEAS, S. Quantification of 5-bromo-2′-deoxyuridine into DNA: an enzyme immunoassay for the assessment of the lymphoid cell proliferative response. J. Immunol. Methods, v.82, p.169-179, 1985.
19. PREZIOSI, P. Dal Cynara scolymus allacito 1,4-dicaffeilchinico. Il Fármaco (ed. Sc.), v. 17, p. 701-745, 1962.
20. STOEV, S.D.; STEFANOV, M.; DENEB, S.; RADIC, B.;, DOMINAN, A.M.; PERAICA, M. Experimental mycotoxicosis in Chickens induced by ochratoxin A and penicillic acid and intervention with natural plant extracts. Vet. Res. Común., v.28, p.724-46, 2004.
21. SUZUKI, T.; ONOGAWA, T.; ASANDO, N.; MIZUTAMARI, H.; MAKKAICHI, T.; TANEMOTO, M.; et al. Identification and characterization of novel rat and human gonad-specific organic anion transporters. Mol. Endocrinol., v.17, p.1203-1215, 2003.
22. WITTEMER, S.M.; PLOCH, M.; WINDECK, T.; MÜLLER, S.C.; DREWELOW, B.; DERENDORF, H.; et al.Bioavailability and pharmacokinetic properties of caffeoylquinic acids and flavonoids after oral administration of artichocke leaf extracts in human. Phytomedicine, v.12, p.25-38, 2005.
23. WOJCICKI, J. Effect of 1,5-dicaffeylquinic acid (cynarine) on colesterol levels in serum and liver of acute ethanol-induced rats. Drug Alc. Dep., v.3, p.143-145, 1978.