메뉴 건너뛰기




Volumn 398, Issue 1, 2010, Pages 68-78

HBsAg-vectored vaccines simultaneously deliver CTL responses to protective epitopes from multiple viral pathogens

Author keywords

Cytotoxic T lymphocyte; Hepatitis B surface antigen; Polyepitope; Recombinant DNA vaccine; Vaccine vector

Indexed keywords

DNA VACCINE; HEPATITIS B SURFACE ANTIGEN; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 1; MULTIEPITOPE 2; POLYEPITOPE 3; RECOMBINANT DNA; UNCLASSIFIED DRUG; VIRUS VECTOR;

EID: 75849159736     PISSN: 00426822     EISSN: 10960341     Source Type: Journal    
DOI: 10.1016/j.virol.2009.11.042     Document Type: Article
Times cited : (3)

References (48)
  • 1
    • 0029965055 scopus 로고    scopus 로고
    • Selective expansion of high- or low-avidity cytotoxic T lymphocytes and efficacy for adoptive immunotherapy
    • Alexander-Miller M.A., Leggatt G.R., and Berzofsky J.A. Selective expansion of high- or low-avidity cytotoxic T lymphocytes and efficacy for adoptive immunotherapy. Proc. Natl. Acad. Sci. U. S. A. 93 (1996) 4102-4107
    • (1996) Proc. Natl. Acad. Sci. U. S. A. , vol.93 , pp. 4102-4107
    • Alexander-Miller, M.A.1    Leggatt, G.R.2    Berzofsky, J.A.3
  • 2
    • 0025879780 scopus 로고
    • Endogenously synthesized peptide with an endoplasmic reticulum signal sequence sensitizes antigen processing mutant cells to class I-restricted cell-mediated lysis
    • Aug 1
    • Anderson K., Cresswell P., Gammon M., Hermes J., Williamson A., and Zweerink H. Endogenously synthesized peptide with an endoplasmic reticulum signal sequence sensitizes antigen processing mutant cells to class I-restricted cell-mediated lysis. J. Exp. Med. 174 2 (1991) 489-492 Aug 1
    • (1991) J. Exp. Med. , vol.174 , Issue.2 , pp. 489-492
    • Anderson, K.1    Cresswell, P.2    Gammon, M.3    Hermes, J.4    Williamson, A.5    Zweerink, H.6
  • 5
    • 1642349618 scopus 로고    scopus 로고
    • Assembly, structure, and antigenic properties of virus-like particles rich in HIV-1 envelope gp120
    • Berkower I., Raymond M., Muller J., Spadaccini A., and Aberdeen A. Assembly, structure, and antigenic properties of virus-like particles rich in HIV-1 envelope gp120. Virology 321 (2004) 75-86
    • (2004) Virology , vol.321 , pp. 75-86
    • Berkower, I.1    Raymond, M.2    Muller, J.3    Spadaccini, A.4    Aberdeen, A.5
  • 6
    • 0037163853 scopus 로고    scopus 로고
    • Recombinant dengue virus type 2 envelope/hepatitis B surface antigen hybrid protein expressed in Pichia pastoris can function as a bivalent immunogen
    • Bisht H., Chugh D.A., Raje M., Swaminathan S.S., and Khanna N. Recombinant dengue virus type 2 envelope/hepatitis B surface antigen hybrid protein expressed in Pichia pastoris can function as a bivalent immunogen. J. Biotechnol. 99 (2002) 97-110
    • (2002) J. Biotechnol. , vol.99 , pp. 97-110
    • Bisht, H.1    Chugh, D.A.2    Raje, M.3    Swaminathan, S.S.4    Khanna, N.5
  • 7
    • 0025770151 scopus 로고
    • Mutational analysis of hepatitis B surface antigen particle assembly and secretion
    • Bruss V., and Ganem D. Mutational analysis of hepatitis B surface antigen particle assembly and secretion. J. Virol. 65 (1991) 3813-3820
    • (1991) J. Virol. , vol.65 , pp. 3813-3820
    • Bruss, V.1    Ganem, D.2
  • 8
    • 0032906065 scopus 로고    scopus 로고
    • Improved immunogenicity of HIV-1 epitopes in HBsAg chimeric DNA vaccine plasmids by structural mutations of HBsAg
    • Bryder K., Sbai H., Nielsen H.V., Corbet S., Nielsen C., Whalen R.G., and Fomsgaard A. Improved immunogenicity of HIV-1 epitopes in HBsAg chimeric DNA vaccine plasmids by structural mutations of HBsAg. DNA Cell Biol. 18 (1999) 219-225
    • (1999) DNA Cell Biol. , vol.18 , pp. 219-225
    • Bryder, K.1    Sbai, H.2    Nielsen, H.V.3    Corbet, S.4    Nielsen, C.5    Whalen, R.G.6    Fomsgaard, A.7
  • 9
    • 0029027693 scopus 로고
    • Selection of T cell receptor variable gene-encoded amino acids on the third binding site loop: a factor influencing variable chain selection in a T cell response
    • Callan M.F., Reyburn H.T., Bowness P., Rowland-Jones S., Bell J.I., and McMichael A.J. Selection of T cell receptor variable gene-encoded amino acids on the third binding site loop: a factor influencing variable chain selection in a T cell response. Eur. J. Immunol. 25 (1995) 1529-1534
    • (1995) Eur. J. Immunol. , vol.25 , pp. 1529-1534
    • Callan, M.F.1    Reyburn, H.T.2    Bowness, P.3    Rowland-Jones, S.4    Bell, J.I.5    McMichael, A.J.6
  • 10
    • 0023777814 scopus 로고
    • Cytotoxic T cells clear virus but augment lung pathology in mice infected with respiratory syncytial virus
    • Cannon M.J., Openshaw P.J., and Askonas B.A. Cytotoxic T cells clear virus but augment lung pathology in mice infected with respiratory syncytial virus. J. Exp. Med. 168 (1988) 1163-1168
    • (1988) J. Exp. Med. , vol.168 , pp. 1163-1168
    • Cannon, M.J.1    Openshaw, P.J.2    Askonas, B.A.3
  • 11
    • 0031935029 scopus 로고    scopus 로고
    • A peptide mimic of a protective epitope of respiratory syncytial virus selected from a combinatorial library induces virus-neutralizing antibodies and reduces viral load in vivo
    • Chargelegue D., Obeid O.E., Hsu S.C., Shaw M.D., Denbury A.N., Taylor G., and Steward M.W. A peptide mimic of a protective epitope of respiratory syncytial virus selected from a combinatorial library induces virus-neutralizing antibodies and reduces viral load in vivo. J. Virol. 72 (1998) 2040-2046
    • (1998) J. Virol. , vol.72 , pp. 2040-2046
    • Chargelegue, D.1    Obeid, O.E.2    Hsu, S.C.3    Shaw, M.D.4    Denbury, A.N.5    Taylor, G.6    Steward, M.W.7
  • 12
    • 27144506549 scopus 로고    scopus 로고
    • Reduced secretion of virions and hepatitis B virus (HBV) surface antigen of a naturally occurring HBV variant correlates with the accumulation of the small S envelope protein in the endoplasmic reticulum and Golgi apparatus
    • Chua P.K., Wang R.Y., Lin M.H., Masuda T., Suk F.M., and Shih C. Reduced secretion of virions and hepatitis B virus (HBV) surface antigen of a naturally occurring HBV variant correlates with the accumulation of the small S envelope protein in the endoplasmic reticulum and Golgi apparatus. J. Virol. 79 (2005) 13483-13496
    • (2005) J. Virol. , vol.79 , pp. 13483-13496
    • Chua, P.K.1    Wang, R.Y.2    Lin, M.H.3    Masuda, T.4    Suk, F.M.5    Shih, C.6
  • 13
    • 0345578684 scopus 로고    scopus 로고
    • A synthetic E7 gene of human papillomavirus type 16 that yields enhanced expression of the protein in mammalian cells and is useful for DNA immunization studies
    • Cid-Arregui A., Juarez V., and Zur H.H. A synthetic E7 gene of human papillomavirus type 16 that yields enhanced expression of the protein in mammalian cells and is useful for DNA immunization studies. J. Virol. 77 (2003) 4928-4937
    • (2003) J. Virol. , vol.77 , pp. 4928-4937
    • Cid-Arregui, A.1    Juarez, V.2    Zur, H.H.3
  • 14
    • 0030174678 scopus 로고    scopus 로고
    • DNA-mediated immunization to hepatitis B surface antigen: longevity of primary response and effect of boost
    • Davis H.L., Mancini M., Michel M.L., and Whalen R.G. DNA-mediated immunization to hepatitis B surface antigen: longevity of primary response and effect of boost. Vaccine 14 (1996) 910-915
    • (1996) Vaccine , vol.14 , pp. 910-915
    • Davis, H.L.1    Mancini, M.2    Michel, M.L.3    Whalen, R.G.4
  • 15
    • 0028864849 scopus 로고
    • DNA-mediated immunization in mice induces a potent MHC class I-restricted cytotoxic T lymphocyte response to the hepatitis B envelope protein
    • Davis H.L., Schirmbeck R., Reimann J., and Whalen R.G. DNA-mediated immunization in mice induces a potent MHC class I-restricted cytotoxic T lymphocyte response to the hepatitis B envelope protein. Hum. Gene Ther. 6 (1995) 1447-1456
    • (1995) Hum. Gene Ther. , vol.6 , pp. 1447-1456
    • Davis, H.L.1    Schirmbeck, R.2    Reimann, J.3    Whalen, R.G.4
  • 16
    • 0032503236 scopus 로고    scopus 로고
    • Mice expressing the E7 oncogene of HPV16 in epithelium show central tolerance, and evidence of peripheral anergising tolerance, to E7-encoded cytotoxic T-lymphocyte epitopes
    • Doan T., Chambers M., Street M., Fernando G.J., Herd K., Lambert P., and Tindle R. Mice expressing the E7 oncogene of HPV16 in epithelium show central tolerance, and evidence of peripheral anergising tolerance, to E7-encoded cytotoxic T-lymphocyte epitopes. Virology 244 (1998) 352-364
    • (1998) Virology , vol.244 , pp. 352-364
    • Doan, T.1    Chambers, M.2    Street, M.3    Fernando, G.J.4    Herd, K.5    Lambert, P.6    Tindle, R.7
  • 19
    • 0026033729 scopus 로고
    • Novel pathways of antigen presentation for the maintenance of memory
    • Gray D., Kosco M., and Stockinger B. Novel pathways of antigen presentation for the maintenance of memory. Int. Immunol. 3 (1991) 141-148
    • (1991) Int. Immunol. , vol.3 , pp. 141-148
    • Gray, D.1    Kosco, M.2    Stockinger, B.3
  • 20
    • 0028884598 scopus 로고
    • Adjuvants for human vaccines-current status, problems and future prospects
    • Gupta R.K., and Siber G.R. Adjuvants for human vaccines-current status, problems and future prospects. Vaccine 13 (1995) 1263-1276
    • (1995) Vaccine , vol.13 , pp. 1263-1276
    • Gupta, R.K.1    Siber, G.R.2
  • 21
    • 35548952747 scopus 로고    scopus 로고
    • Multiple copies of a tumor epitope in a recombinant hepatitis B surface antigen (HBsAg) vaccine enhance CTL responses, but not tumor protection
    • Haigh O., Guo H., Edgtton K., Mather M., Herd K.A., and Tindle R.W. Multiple copies of a tumor epitope in a recombinant hepatitis B surface antigen (HBsAg) vaccine enhance CTL responses, but not tumor protection. Virology 368 (2007) 363-375
    • (2007) Virology , vol.368 , pp. 363-375
    • Haigh, O.1    Guo, H.2    Edgtton, K.3    Mather, M.4    Herd, K.A.5    Tindle, R.W.6
  • 22
    • 32444432815 scopus 로고    scopus 로고
    • Cytotoxic T-lymphocyte epitope vaccination protects against human metapneumovirus infection and disease in mice
    • Herd K.A., Mahalingam S., Mackay I.M., Nissen M., Sloots T.P., and Tindle R.W. Cytotoxic T-lymphocyte epitope vaccination protects against human metapneumovirus infection and disease in mice. J. Virol. 80 (2006) 2034-2044
    • (2006) J. Virol. , vol.80 , pp. 2034-2044
    • Herd, K.A.1    Mahalingam, S.2    Mackay, I.M.3    Nissen, M.4    Sloots, T.P.5    Tindle, R.W.6
  • 23
    • 34247872819 scopus 로고    scopus 로고
    • Cellular responses to viral infection in humans: lessons from Epstein-Barr virus
    • Hislop A.D., Taylor G.S., Sauce D., and Rickinson A.B. Cellular responses to viral infection in humans: lessons from Epstein-Barr virus. Annu. Rev. Immunol. 25 (2007) 587-617
    • (2007) Annu. Rev. Immunol. , vol.25 , pp. 587-617
    • Hislop, A.D.1    Taylor, G.S.2    Sauce, D.3    Rickinson, A.B.4
  • 24
    • 0032518481 scopus 로고    scopus 로고
    • Reduction of respiratory syncytial virus titer in the lungs of mice after intranasal immunization with a chimeric peptide consisting of a single CTL epitope linked to a fusion peptide
    • Hsu S.C., Chargelegue D., and Steward M.W. Reduction of respiratory syncytial virus titer in the lungs of mice after intranasal immunization with a chimeric peptide consisting of a single CTL epitope linked to a fusion peptide. Virology 240 (1998) 376-381
    • (1998) Virology , vol.240 , pp. 376-381
    • Hsu, S.C.1    Chargelegue, D.2    Steward, M.W.3
  • 26
    • 0036172967 scopus 로고    scopus 로고
    • Virus-specific CTL responses induced by an H-2K(d)-restricted, motif-negative 15-mer peptide from the fusion protein of respiratory syncytial virus
    • Jiang S., Borthwick N.J., Morrison P., Gao G.F., and Steward M.W. Virus-specific CTL responses induced by an H-2K(d)-restricted, motif-negative 15-mer peptide from the fusion protein of respiratory syncytial virus. J. Gen. Virol. 83 (2002) 429-438
    • (2002) J. Gen. Virol. , vol.83 , pp. 429-438
    • Jiang, S.1    Borthwick, N.J.2    Morrison, P.3    Gao, G.F.4    Steward, M.W.5
  • 27
    • 0042905865 scopus 로고    scopus 로고
    • DNA vaccination: the answer to stable, protective T-cell memory?
    • Kirman J.R., and Seder R.A. DNA vaccination: the answer to stable, protective T-cell memory?. Curr. Opin. Immunol. 15 (2003) 471-476
    • (2003) Curr. Opin. Immunol. , vol.15 , pp. 471-476
    • Kirman, J.R.1    Seder, R.A.2
  • 29
    • 0031215046 scopus 로고    scopus 로고
    • The preferential induction of a Th1 immune response by DNA-based immunization is mediated by the immunostimulatory effect of plasmid DNA
    • Leclerc C., Deriaud E., Rojas M., and Whalen R.G. The preferential induction of a Th1 immune response by DNA-based immunization is mediated by the immunostimulatory effect of plasmid DNA. Cell Immunol. 179 (1997) 97-106
    • (1997) Cell Immunol. , vol.179 , pp. 97-106
    • Leclerc, C.1    Deriaud, E.2    Rojas, M.3    Whalen, R.G.4
  • 30
    • 0030068375 scopus 로고    scopus 로고
    • Treatment of established tumors with a novel vaccine that enhances major histocompatibility class II presentation of tumor antigen
    • Lin K.Y., Guarnieri F.G., Staveley-O'Carroll K.F., Levitsky H.I., August J.T., Pardoll D.M., and Wu T.C. Treatment of established tumors with a novel vaccine that enhances major histocompatibility class II presentation of tumor antigen. Cancer Res. 56 (1996) 21-26
    • (1996) Cancer Res. , vol.56 , pp. 21-26
    • Lin, K.Y.1    Guarnieri, F.G.2    Staveley-O'Carroll, K.F.3    Levitsky, H.I.4    August, J.T.5    Pardoll, D.M.6    Wu, T.C.7
  • 31
    • 0035860626 scopus 로고    scopus 로고
    • Optimization of epitope processing enhances immunogenicity of multiepitope DNA vaccines
    • Livingston B.D., Newman M., Crimi C., McKinney D., Chesnut R., and Sette A. Optimization of epitope processing enhances immunogenicity of multiepitope DNA vaccines. Vaccine 19 (2001) 4652-4660
    • (2001) Vaccine , vol.19 , pp. 4652-4660
    • Livingston, B.D.1    Newman, M.2    Crimi, C.3    McKinney, D.4    Chesnut, R.5    Sette, A.6
  • 32
    • 0034666835 scopus 로고    scopus 로고
    • Multiepitopic HLA-A*0201-restricted immune response against hepatitis B surface antigen after DNA-based immunization
    • Loirat D., Lemonnier F.A., and Michel M.L. Multiepitopic HLA-A*0201-restricted immune response against hepatitis B surface antigen after DNA-based immunization. J. Immunol. 165 (2000) 4748-4755
    • (2000) J. Immunol. , vol.165 , pp. 4748-4755
    • Loirat, D.1    Lemonnier, F.A.2    Michel, M.L.3
  • 33
    • 33846602343 scopus 로고    scopus 로고
    • Optimisation of secretion of recombinant HBsAg virus-like particles: impact on the development of HIV-1/HBV bivalent vaccines
    • Michel M., Lone Y.C., Centlivre M., Roux P., Wain-Hobson S., and Sala M. Optimisation of secretion of recombinant HBsAg virus-like particles: impact on the development of HIV-1/HBV bivalent vaccines. Vaccine 25 (2007) 1901-1911
    • (2007) Vaccine , vol.25 , pp. 1901-1911
    • Michel, M.1    Lone, Y.C.2    Centlivre, M.3    Roux, P.4    Wain-Hobson, S.5    Sala, M.6
  • 34
    • 0035124015 scopus 로고    scopus 로고
    • Antigenicity and immunogenicity of novel chimeric hepatitis B surface antigen particles with exposed hepatitis C virus epitopes
    • Netter H.J., Macnaughton T.B., Woo W.P., Tindle R., and Gowans E.J. Antigenicity and immunogenicity of novel chimeric hepatitis B surface antigen particles with exposed hepatitis C virus epitopes. J. Virol. 75 (2001) 2130-2141
    • (2001) J. Virol. , vol.75 , pp. 2130-2141
    • Netter, H.J.1    Macnaughton, T.B.2    Woo, W.P.3    Tindle, R.4    Gowans, E.J.5
  • 35
    • 0032954943 scopus 로고    scopus 로고
    • Differential binding of viral peptides to HLA-A2 alleles. Implications for human papillomavirus type 16 E7 peptide-based vaccination against cervical carcinoma
    • Ressing M.E., de Jong J.H., Brandt R.M., Drijfhout J.W., Benckhuijsen W.E., Schreuder G.M., Offringa R., Kast W.M., and Melief C.J. Differential binding of viral peptides to HLA-A2 alleles. Implications for human papillomavirus type 16 E7 peptide-based vaccination against cervical carcinoma. Eur. J. Immunol. 29 (1999) 1292-1303
    • (1999) Eur. J. Immunol. , vol.29 , pp. 1292-1303
    • Ressing, M.E.1    de Jong, J.H.2    Brandt, R.M.3    Drijfhout, J.W.4    Benckhuijsen, W.E.5    Schreuder, G.M.6    Offringa, R.7    Kast, W.M.8    Melief, C.J.9
  • 36
    • 38549175830 scopus 로고    scopus 로고
    • DNA vaccines: precision tools for activating effective immunity against cancer
    • Rice J., Ottensmeier C.H., and Stevenson F.K. DNA vaccines: precision tools for activating effective immunity against cancer. Nat. Rev. Cancer 8 (2008) 108-120
    • (2008) Nat. Rev. Cancer , vol.8 , pp. 108-120
    • Rice, J.1    Ottensmeier, C.H.2    Stevenson, F.K.3
  • 37
    • 24144444534 scopus 로고    scopus 로고
    • Clinical safety and efficacy of a powdered Hepatitis B nucleic acid vaccine delivered to the epidermis by a commercial prototype device
    • Roberts L.K., Barr L.J., Fuller D.H., McMahon C.W., Leese P.T., and Jones S. Clinical safety and efficacy of a powdered Hepatitis B nucleic acid vaccine delivered to the epidermis by a commercial prototype device. Vaccine 23 (2005) 4867-4878
    • (2005) Vaccine , vol.23 , pp. 4867-4878
    • Roberts, L.K.1    Barr, L.J.2    Fuller, D.H.3    McMahon, C.W.4    Leese, P.T.5    Jones, S.6
  • 38
    • 0035902080 scopus 로고    scopus 로고
    • Progress in human tumor immunology and immunotherapy
    • Rosenberg S.A. Progress in human tumor immunology and immunotherapy. Nature 411 (2001) 380-384
    • (2001) Nature , vol.411 , pp. 380-384
    • Rosenberg, S.A.1
  • 40
    • 0037135675 scopus 로고    scopus 로고
    • Role of transfection in the priming of cytotoxic T-cells by DNA-mediated immunization
    • Sbai H., Schneider J., Hill A.V., and Whalen R.G. Role of transfection in the priming of cytotoxic T-cells by DNA-mediated immunization. Vaccine 20 (2002) 3137-3147
    • (2002) Vaccine , vol.20 , pp. 3137-3147
    • Sbai, H.1    Schneider, J.2    Hill, A.V.3    Whalen, R.G.4
  • 41
    • 0029101242 scopus 로고
    • Nucleic acid vaccination primes hepatitis B virus surface antigen-specific cytotoxic T lymphocytes in nonresponder mice
    • Schirmbeck R., Bohm W., Ando K., Chisari F.V., and Reimann J. Nucleic acid vaccination primes hepatitis B virus surface antigen-specific cytotoxic T lymphocytes in nonresponder mice. J. Virol. 69 (1995) 5929-5934
    • (1995) J. Virol. , vol.69 , pp. 5929-5934
    • Schirmbeck, R.1    Bohm, W.2    Ando, K.3    Chisari, F.V.4    Reimann, J.5
  • 42
    • 0028299207 scopus 로고
    • Immunization with soluble hepatitis B virus surface protein elicits murine H-2 class I-restricted CD8+ cytotoxic T lymphocyte responses in vivo
    • Schirmbeck R., Melber K., Kuhrober A., Janowicz Z.A., and Reimann J. Immunization with soluble hepatitis B virus surface protein elicits murine H-2 class I-restricted CD8+ cytotoxic T lymphocyte responses in vivo. J. Immunol. 152 (1994) 1110-1119
    • (1994) J. Immunol. , vol.152 , pp. 1110-1119
    • Schirmbeck, R.1    Melber, K.2    Kuhrober, A.3    Janowicz, Z.A.4    Reimann, J.5
  • 43
  • 46
    • 0018647438 scopus 로고
    • Nucleotide sequence of the gene coding for the major protein of hepatitis B virus surface antigen
    • Valenzuela P., Gray P., Quiroga M., Zaldivar J., Goodman H.M., and Rutter W.J. Nucleotide sequence of the gene coding for the major protein of hepatitis B virus surface antigen. Nature 280 (1979) 815-819
    • (1979) Nature , vol.280 , pp. 815-819
    • Valenzuela, P.1    Gray, P.2    Quiroga, M.3    Zaldivar, J.4    Goodman, H.M.5    Rutter, W.J.6
  • 47
    • 0025729554 scopus 로고
    • Analysis of the HLA-restricted influenza-specific cytotoxic T lymphocyte response in transgenic mice carrying a chimeric human-mouse class I major histocompatibility complex
    • Vitiello A., Marchesini D., Furze J., Sherman L.A., and Chesnut R.W. Analysis of the HLA-restricted influenza-specific cytotoxic T lymphocyte response in transgenic mice carrying a chimeric human-mouse class I major histocompatibility complex. J. Exp. Med. 173 (1991) 1007-1015
    • (1991) J. Exp. Med. , vol.173 , pp. 1007-1015
    • Vitiello, A.1    Marchesini, D.2    Furze, J.3    Sherman, L.A.4    Chesnut, R.W.5
  • 48
    • 33645787828 scopus 로고    scopus 로고
    • Hepatitis B surface antigen vector delivers protective cytotoxic T-lymphocyte responses to disease-relevant foreign epitopes
    • Woo W.P., Doan T., Herd K.A., Netter H.J., and Tindle R.W. Hepatitis B surface antigen vector delivers protective cytotoxic T-lymphocyte responses to disease-relevant foreign epitopes. J. Virol. 80 (2006) 3975-3984
    • (2006) J. Virol. , vol.80 , pp. 3975-3984
    • Woo, W.P.1    Doan, T.2    Herd, K.A.3    Netter, H.J.4    Tindle, R.W.5


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.