In vitro selection of ssDNA aptamers using biotinylated target proteins

Methods in Molecular Biology

Volumn 535, Issue , 2009, Pages 19-32

  Mayer, Günter ^a   Höver, Thomas ^a  

^a UNIVERSITY OF BONN   (Germany)

Author keywords

aptamers; biotin; in vitro selection; SELEX; ssDNA; streptavidin

Indexed keywords

APTAMER; BIOTIN; PROTEIN; SINGLE STRANDED DNA; STREPTAVIDIN; THROMBIN;

ARTICLE; BIOTINYLATION; CAPILLARY ELECTROPHORESIS; CHEMISTRY; COMBINATORIAL CHEMISTRY; GENETICS; HUMAN; ISOTOPE LABELING; METABOLISM; METHODOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS;

APTAMERS, NUCLEOTIDE; BIOTIN; BIOTINYLATION; DNA, SINGLE-STRANDED; ELECTROPHORESIS, CAPILLARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; ISOTOPE LABELING; PROTEINS; SELEX APTAMER TECHNIQUE; STREPTAVIDIN; THROMBIN;

EID: 75749141091     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-557-2_2     Document Type: Article

References (12)

1. Famulok, M., Hartig, J.S. and Mayer, G. (2007) Functional aptamers and aptazymes in biotechnology, diagnostics, and therapy. Chem. Rev.107, 3715-3743. (Pubitemid 47534316)
2. Ellington, A.D. and Szostak, J.W. (1990) In vitro selection of RNA molecules that bind specific ligands. Nature (London) 346, 818-821.
3. Müller, J., Wulffen, B., Pötzsch, B. and Mayer, G. (2007) Multi-domain targeting generates a high affinity thrombin-inhibiting bivalent aptamer. ChemBioChem 8, 2223-2226.
4. Mayer, G. and Jenne, A. (2004) Aptamers in research and drug development. BioDrugs 6, 351-359. (Pubitemid 40023610)
5. Bock, L.C., Griffin, L.C. Latham, J.A., Vermaas, E.H. and Toole, J.J. (1992) Selection of single stranded DNA molecules that bind and inhibit human thrombin. Nature 355, 564-566.
6. Mayer, G., Wulffen, B., Huber, C., Brock-mann, J., Flicke, B., Neumann, L., Hafen-bradl, D., Klebl, B.M., Lohse, M.J., Krasel, C. and Blind, M. (2008) An RNA molecule that specifically inhibits G-protein coupled receptor kinase 2 in vitro. RNA 14, 524-534. (Pubitemid 351397817)
7. Schurer, H., Stembera, K., Knoll, D., Mayer, G., Blind, M., Forster, H., Famulok, M., Welzel, P. and Hahn, U. (2001) Apta-mers that bind to the antibiotic moenomycin A. Bioorg. Med. Chem. 9, 2557-2563. (Pubitemid 32824158)
8. Raddatz, M.-S.L., Dolf, A., Knolle, P., Endl, E., Famulok, M. and Mayer, G. (2008) Enrichment of cell-targeting and population-specific aptamers by fluorescent-activated cell-sorting. Angew. Chem. Int. Ed. 47, 5190-5193.
9. Famulok, M., Mayer, G. and Blind, M. (2000) Nucleic acid aptamers-From selection in vitro to application in vivo. Acc. Chem. Res. 33, 591-99.
10. Wang, K.Y., Krawczyk, S. H., Bischofberger, N., Swaminatham, S. and Bolton, P.H. (1993) The tertiary structure of a DNA aptamer which binds to and inhibits throm-bin determines activity. Biochemistry 32, 11285-11292. (Pubitemid 23332039)
11. Li, W.X., Kaplan, A.V., Grant, G.W., Toole, J.J. and Leung, L.L.K. (1994) A novel nucleotide-based thrombin inhibitor inhibits clot-bound thrombin and reduces arterial platelet thrombus formation. Blood 83, 677-682. (Pubitemid 24043727)
12. Müller, J., El-Maarri, O., Oldenburg, J., Pötzsch, B. and Mayer, G. (2008) Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography. Anal. Bioanal. Chem. 390, 1033-1037.