The AC133+CD38-, but not the rhodamine-low, phenotype tracks LTC-IC and SRC function in human cord blood ex vivo expansion cultures

Blood

Volumn 115, Issue 2, 2010, Pages 257-260

  Ito, Caryn Y ^a   Kirouac, Daniel C ^a   Madlambayan, Gerard J ^b   Yu, Mei ^a   Rogers, Ian ^c   Zandstra, Peter W ^a,d,e,f  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF FLORIDA COLLEGE OF MEDICINE   (United States)

^c MOUNT SINAI HOSPITAL   (Canada)

^d UNIVERSITY OF TORONTO   (Canada)

^e UNIVERSITY OF TORONTO   (Canada)

^f UNIVERSITY HEALTH NETWORK   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CD133 ANTIGEN; CD38 ANTIGEN; RHODAMINE; CD38 PROTEIN, HUMAN; GLYCOPROTEIN; LEUKOCYTE ANTIGEN; MEMBRANE PROTEIN; PEPTIDE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIGEN EXPRESSION; ARTICLE; CELL CULTURE; CELL ISOLATION; CELL SUBPOPULATION; CONTROLLED STUDY; DILUTION; EX VIVO STUDY; GENE EXPRESSION; HEMATOPOIETIC STEM CELL; HUMAN; MOUSE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROSPECTIVE STUDY; SCID MOUSE; SEVERE COMBINED IMMUNODEFICIENCY; UMBILICAL CORD BLOOD; ANIMAL; BIOSYNTHESIS; CULTURE TECHNIQUE; CYTOLOGY; FETUS BLOOD; GENE EXPRESSION REGULATION; HEMATOPOIETIC STEM CELL TRANSPLANTATION; METABOLISM; MOUSE MUTANT; NONOBESE DIABETIC MOUSE; PHYSIOLOGY; XENOGRAFT;

ANIMALS; ANTIGENS, CD; ANTIGENS, CD38; CELL CULTURE TECHNIQUES; CELLS, CULTURED; FETAL BLOOD; GENE EXPRESSION REGULATION; GLYCOPROTEINS; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HEMATOPOIETIC STEM CELLS; HUMANS; MEMBRANE GLYCOPROTEINS; MICE; MICE, INBRED NOD; MICE, SCID; PEPTIDES; TRANSPLANTATION, HETEROLOGOUS;

EID: 75649124978     PISSN: 00064971     EISSN: 15280020     Source Type: Journal    
DOI: 10.1182/blood-2009-07-228106     Document Type: Article

References (17)

1. - cells in lymphohematopoiesis. Leuk Lymphoma. 1998;31(3):285-293.
2. - cells in ex vivo culture during retroviral transduction without a corresponding increase in SCID repopulating cell (SRC) frequency: dissociation of SRC phenotype and function. Blood. 2000;95(1):102-110.
3. Madlambayan GJ, Rogers I, Kirouac DC, et al. Dynamic changes in cellular and microenvironmental composition can be controlled to elicit in vitro human hematopoietic stem cell expansion. Exp Hematol. 2005;33(10):1229-1239.
4. Chaudhary PM, Roninson IB. Expression and activity of P-glycoprotein, a multidrug efflux pump, in human hematopoietic stem cells. Cell. 1991;66(1):85-94.
5. Uchida N, Combs J, Chen S, Zanjani E, Hoffman R, Tsukamoto A. Primitive human hematopoietic cells displaying differential efflux of the rhodamine 123 dye have distinct biological activities. Blood. 1996;88(4):1297-1305.
6. Udomsakdi C, Eaves CJ, Sutherland HJ, Lansdorp PM. Separation of functionally distinct subpopulations of primitive human hematopoietic cells using rhodamine-123. Exp Hematol. 1991; 19(5):338-342.
7. Baum CM, Weissman IL, Tsukamoto AS, Buckle AM, Peault B. Isolation of a candidate human hematopoietic stem-cell population. Proc Natl Acad Sci U S A. 1992;89(7):2804-2808.
8. Miraglia S, Godfrey W, Yin AH, et al. A novel fivetransmembrane hematopoietic stem cell antigen: isolation, characterization, and molecular cloning. Blood. 1997;90(12):5013-5021.
9. Yin AH, Miraglia S, Zanjani ED, et al. AC133, a novel marker for human hematopoietic stem and progenitor cells. Blood. 1997;90(12):5002-5012.
10. Uchida N, Buck DW, He D, et al. Direct isolation of human central nervous system stem cells. Proc Natl Acad Sci U S A. 2000;97(26):14720-14725.
11. Sugiyama T, Rodriguez RT, McLean GW, Kim SK. Conserved markers of fetal pancreatic epithelium permit prospective isolation of islet progenitor cells by FACS. Proc Natl Acad Sci U S A. 2007; 104(1):175-180.
12. O'Brien CA, Pollett A, Gallinger S, Dick JE. A human colon cancer cell capable of initiating tumour growth in immunodeficient mice. Nature. 2007; 445(7123):106-110.
13. Ricci-Vitiani L, Lombardi DG, Pilozzi E, et al. Identification and expansion of human colon-cancer-initiating cells. Nature. 2007;445(7123):111- 115.
14. - population. Blood. 2007; 109(2):543-545.
15. Cox CV, Diamanti P, Evely RS, Kearns PR, Blair A. Expression of CD133 on leukemia-initiating cells in childhood ALL. Blood. 2009;113(14):3287-3296.
16. Madlambayan GJ, Rogers I, Purpura KA, et al. Clinically relevant expansion of hematopoietic stem cells with conserved function in a singleuse, closed-system bioprocess. Biol Blood Marrow Transplant. 2006;12(10):1020-1030.
17. Bhatia M, Bonnet D, Murdoch B, Gan OI, Dick JE. A newly discovered class of human hematopoietic cells with SCID-repopulating activity. Nat Med. 1998;4(9):1038-1045.