Purification and some properties of wild-type and N-terminal-truncated ethanolamine ammonia-lyase of Escherichia coli

Journal of Biochemistry

Volumn 147, Issue 1, 2010, Pages 83-93

  Akita, Keita ^a   Hieda, Naoki ^a   Baba, Nobuyuki ^a   Kawaguchi, Satoshi ^a   Sakamoto, Hirohisa ^a   Nakanishi, Yuka ^a   Yamanishi, Mamoru ^a   Mori, Koichi ^a   Toraya, Tetsuo ^a  

^a OKAYAMA UNIVERSITY   (Japan)

Author keywords

Adenosylcobalamin; Coenzyme B12; Ethanolamine ammonia lyase; Radical enzyme; Truncated enzyme

Indexed keywords

ETHANOLAMINE AMMONIA LYASE;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL GENE; CATALYSIS; ELECTRON SPIN RESONANCE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME STABILITY; ESCHERICHIA COLI; EUTB GENE; EUTC GENE; NONHUMAN; OPERON; SOLUBILITY; STRUCTURE ACTIVITY RELATION;

CATALYSIS; ENZYME STABILITY; ESCHERICHIA COLI; ETHANOLAMINE AMMONIA-LYASE; RECOMBINANT PROTEINS; SOLUBILITY;

ESCHERICHIA COLI;

EID: 75649097075     PISSN: 0021924X     EISSN: 17562651     Source Type: Journal    
DOI: 10.1093/jb/mvp145     Document Type: Article

References (46)

1. Bradbeer, C. (1965) The clostridial fermentations of choline and ethanolamine. II. Requirement for a cobamide coenzyme by an ethanolamine deaminase. J. Biol. Chem. 240, 4675-4681
2. Bradbeer, C. (1965) The clostridial fermentations of choline and ethanolamine. I. Preparation and properties of cell-free extracts. J. Biol. Chem. 240, 4669-4674
3. 12- dependent enzyme ethanolamine deaminase in Salmonella. Nature 95, 150-151
4. 12-dependent ethanolamine ammonia-lyase in Escherichia coli and Klebsiella aerogenes. J. Gen. Microbiol. 95, 173-176
5. Wolf, J.B. and Brey, R.N. (1986) Isolation and genetic characterizations of Bacillus megaterium cobalamin biosynthesis- deficient mutants. J. Bacteriol. 166, 51-58
6. Roof, D.M. and Roth, J.R. (1988) Ethanolamine utilization in Salmonella typhimurium. J. Bacteriol. 170, 3855-3863
7. Frings, J., Wondrak, C., and Schink, B. (1994) Fermentative degradation of triethanolamine by a homoacetogenic bacterium. Arch. Microbiol. 162, 103-107
8. Kaplan, B.H. and Stadtman, E.R. (1968) Ethanolamine deaminase, a cobamide coenzyme-dependent enzyme. I. Purification, assay, and properties of the enzyme. J. Biol. Chem. 243, 1787-1793
9. 12-dependent ethanolamine ammonialyase of Escherichia coli. Biochem. J. 175, 555-563
10. Wallis, O.C., Johnson, A.W., and Lappert, M.F. (1979) Studies on the subunit structure of the adenosylcobalamin- dependent enzyme ethanolamine ammonia-lyase. FEBS Lett. 97, 196-199
11. Faust, L.P. and Babior, B.M. (1992) Overexpression, purification, and some properties of the AdoCbldependent ethanolamine ammonia-lyase from Salmonella typhimurium. Arch. Biochem. Biophys. 294, 50-54
12. Kaplan, B.H. and Stadtman, E.R. (1968) Ethanolamine deaminase, a cobamide coenzyme-dependent enzyme. II. Physical and chemical properties and interaction with cobamides and ethanolamine. J. Biol. Chem. 243, 1794-1803
13. Sheppard, D.E. and Roth, J.R. (1994) A rationale for autoinduction of a transcriptional activator: ethanolamine ammonia-lyase (EutBC) and the operon activator (EutR) compete for adenosyl-cobalamin in Salmonella typhimurium. J. Bacteriol. 176, 1287-1296
14. Kofoid, E., Rappleye, C., Stojiljkovic, I., and Roth, J. (1999) The 17-gene ethanolamine (eut) operon of Salmonella typhimurium encodes five homologues of carboxysome shell proteins. J. Bacteriol. 181, 5317-5329
15. 12 (Dolphin D., ed.) Vol.2, pp. 263-287, John Wiley & Sons, New York
16. 12 (Banerjee R., ed.), pp. 811-833, John Wiley & Sons, New York
17. Reed, G.H. (2004) Radical mechanisms in adenosylcobalamin- dependent enzymes. Curr. Opin. Chem. Biol. 8, 477-483
18. Sando, G.N., Grant, M.E., and Hogenkamp, H.P.C. (1976) The interaction of adeninylalkylcobalamins with ribonucleotide reductase. Biochim. Biophys. Acta 428, 228-232
19. Toraya, T. and Ishida, A. (1991) Roles of the D-ribose and 5,6-dimethylbenzimidazole moieties of the nucleotide loop of adenosylcobalamin in manifestation of coenzymic function in the diol dehydrase reaction. J. Biol. Chem. 266, 5430-5437
20. Yamanishi, M., Yamada, S., Muguruma, H., Murakami, Y., Tobimatsu, T., Ishida, A., Yamauchi, J., and Toraya, T. (1998) Evidence for axial coordination of 5,6-dimethylbenzimidazole to the cobalt atom of adenosylcobalamin bound to diol dehydratase. Biochemistry 37, 4799-4803
21. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. 2nd edn., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
22. Kohara, Y., Akiyama, K., and Isono, K. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50, 495-508
23. Tobimatsu, T., Kawata, M., and Toraya, T. (2005) The N-terminal regions of b and g subunits lower the solubility of adenosylcobalamin-dependent diol dehydratase. Biosci. Biotechnol. Biochem. 69, 455-462
24. Mori, K., Bando, R., Hieda, N., and Toraya, T. (2004) Identification of a reactivating factor for adenosylcobalamin- dependent ethanolamine ammonia lyase. J. Bacteriol. 186, 6845-6854
25. Toraya, T., Ushio, K., Fukui, S., and Hogenkamp, H.P.C. (1977) Studies on the mechanism of the adenosylcobalamin-dependent diol dehydrase reaction by the use of analogs of the coenzyme. J. Biol. Chem. 252, 963-970
26. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275
27. Gill, S.C. and von Hippel, P.H. (1989) Calculation of protein extinction coefficients from amino acid sequence data. Anal. Biochem. 182, 319-326
28. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685
29. Babior, B.M. and Li, T.K. (1969) The binding of cobamides to ethanolamine deaminase. Biochemistry 8, 154-160
30. Hollaway, M.R., Johnson, A.W., Lappert, M.F., and Wallis, O.C. (1980) The number of functional active sites per molecule of the adenosylcobalamin- dependent enzyme, ethanolamine ammonia-lyase, as determined by a kinetic method. Eur. J. Biochem. 111, 177-188
31. Bandarian, V. and Reed, G.H. (1999) Hydrazine cation radical in the active site of ethanolamine ammonia-lyase: mechanism-based inactivation by hydroxyethylhydrazine. Biochemistry 38, 12394-12402
32. Krouwer, J.S., Holmquist, B., Kipnes, R.S., and Babior, B.M. (1980) The mechanism of action of ethanolamine ammonia-lyase, an adenosylcobalamindependent enzyme. Evidence that carbon-cobalt bond cleavage is driven in part by conformational alterations of the corrin ring. Biochim. Biophys. Acta 612, 153-159
33. 12-dependent enzyme. The participation of paramagnetic species in the catalytic deamination of 2-aminopropanol. J. Biol. Chem. 249, 4537-4544
34. 12 coenzyme-dependent ethanolamine deaminase catalysis. J. Am. Chem. Soc. 121, 10522-10528
35. Bender, G., Poyner, R.R., and Reed, G.H. (2008) Identification of the substrate radical intermediate derived from ethanolamine during catalysis by ethanolamine ammonia-lyase. Biochemistry 47, 11360-11366
36. 12-dependent ethanolamine ammonia-lyase catalyzed reaction of S-2- aminopropanol. Biochemistry 41, 8580-8588
37. 12-dependent enzyme reactions. J. Chem. Soc., Faraday Trans. 2 74, 417-431
38. Buettner, G.R. and Coffman, R.E. (1977) EPR determination of the Co(II)-free radical magnetic geometry of the "doublet" species arising in a coenzyme B-12-enzyme reaction. Biochim. Biophys. Acta 480, 495-505
39. 12. Biochim. Biophys. Acta 397, 510-518
40. 12 in the "base-on" mode: ESR investigations on cob(II)alamin. Angew. Chem. Int. Ed. 37, 625-627
41. 12 binding and the direct participation of a potassium ion in enzyme catalysis: X-ray structure of diol dehydratase. Structure 7, 997-1008
42. 12-binding domains of methionine synthase. Science 266, 1669-1674
43. 12. Arch. Biochem. Biophys. 370, 138-141
44. Ke, S.-C., Torrent, M., Museav, D.G., Morokuma, K., and Warncke, K. (1999) Identification of dimethylbenzimidazole axial coordination and characterization of 14N superhyperfine and nuclear quadrupole coupling in cob(II)alamin bound to ethanolamine deaminase in a catalytically-engaged substrate radical-cobalt(II) biradical state. Biochemistry 38, 12681-12689
45. 12-dependent enzyme. The reversible formation of 50-deoxyadenosine from adenosylcobalamin during the catalytic process. J. Biol. Chem. 249, 1689-1695
46. Mauck, L. and Babior, B.M. (1977) Inactivation of ethanolamine ammonia-lyase by 5,50-dithiobis(2- nitrobenzoic acid). Further evidence for the involvement of sulfhydryl groups in adenosylcobalamin-dependent rearrangements. Biochim. Biophys. Acta. 480, 489-494