Expression of GFP under the control of the RNA helicase VASA permits fluorescence-activated cell sorting isolation of human primordial germ cells

Stem Cells

Volumn 28, Issue 1, 2010, Pages 84-92

  Tilgner, Katarzyna ^a   Atkinson, Stuart P ^a   Yung, Sun ^a   Golebiewska, Anna ^a   Stojkovic, Miodrag ^b   Moreno, Ruben ^b   Lako, Majlinda ^a   Armstrong, Lyle ^a  

^a NEWCASTLE UNIVERSITY   (United Kingdom)

^b CENTRO DE INVESTIGACIÓN PRÍNCIPE FELIPE   (Spain)

Author keywords

Embryonic stem cells; Meiosis; Primordial germ cells; VASA

Indexed keywords

GREEN FLUORESCENT PROTEIN; RNA HELICASE;

ARTICLE; CELL DIFFERENTIATION; CELL ISOLATION; EMBRYONIC STEM CELL; FLUORESCENCE ACTIVATED CELL SORTING; GENE CONSTRUCT; HUMAN; HUMAN CELL; PRIMORDIAL GERM CELL; PROTEIN EXPRESSION; REPORTER GENE;

CELL DIFFERENTIATION; CELL LINE; CELL SEPARATION; CHROMATIN ASSEMBLY AND DISASSEMBLY; DEAD-BOX RNA HELICASES; EMBRYONIC STEM CELLS; FEMALE; FLOW CYTOMETRY; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GERM CELLS; GREEN FLUORESCENT PROTEINS; HUMANS; MEIOSIS; MICROSCOPY, CONFOCAL; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TIME FACTORS; TRANSFECTION;

MUS MUSCULUS;

EID: 75349103553     PISSN: 10665099     EISSN: None     Source Type: Journal    
DOI: 10.1002/stem.263     Document Type: Article

References (40)

1. Johnson L, Staub C, Neaves WB et al. Live human germ cells in the context of their spermatogenic stages. Hum Reprod 2001;16(8): 1575-1582.
2. Heyn R, Makabe S, Motta PM. Ultrastructural dynamics of human testicular cords from 6 to 16 weeks of embryonic development. Study by transmission and high resolution scanning electron microscopy. Ital J Anat Embryol 1998;103(4 Suppl 1):17-29.
3. Pesce M, Gioia Klinger F, De Felici M. Derivation in culture of primordial germ cells from cells of the mouse epiblast: Phenotypic induction and growth control by Bmp4 signalling. Mech Dev 2002; 112(1-2):15-24.
4. West JA, Park IH, Daley GQ et al. In vitro generation of germ cells from murine embryonic stem cells. Nat Protoc 2006;1(4):2026-2036.
5. Toyooka Y, Tsunekawa N, Akasu R et al. Embryonic stem cells can form germ cells in vitro. Proc Natl Acad Sci U S A 2003;100(20): 11457-11462.
6. Hübner K, Fuhrmann G, Christenson LK et al. Derivation of oocytes from mouse embryonic stem cells. Science 2003;300(5623):1251-1256.
7. Clark AT, Bodnar MS, Fox M et al. Spontaneous differentiation of germ cells from human embryonic stem cells in vitro. Hum Mol Genet 2004; 13(7):727-739.
8. Kierszenbaum AL. Genomic imprinting and epigenetic reprogramming: Unearthing the garden of forking paths. Mol Reprod Dev 2002; 63(3):269-272.
9. Sato S, Yoshimizu T, Sato E et al. Erasure of methylation imprinting of Igf2r during mouse primordial germ-cell development. Mol Reprod Dev 2003;65(1):41-50.
10. Tilgner K, Atkinson SP, Golebiewska A et al. Isolation of primordial germ cells from differentiating human embryonic stem cells. Stem Cells 2008;26(12):3075-3085.
11. Capela A, Temple S. LeX is expressed by principle progenitor cells in the embryonic nervous system, is secreted into their environment and binds Wnt-1. Dev Biol 2006;291(2):300-313.
12. Castrillon DH, Quade BJ, Wang TY et al. The human VASA gene is specifically expressed in the germ cell lineage. Proc Natl Acad Sci U S A 2000;97(17):9585-9590.
13. Ikenishi K, Tanaka TS. Spatio-temporal expression of Xenopus vasa homolog, XVLG1, in oocytes and embryos: The presence of XVLG1 RNA in somatic cells as well as germline cells. Dev Growth Differ 2000;42(2):95-103.
14. Bártfai R, Orbán L. The vasa locus in zebrafish: Multiple RGG boxes from duplications. DNA Cell Biol 2003;22(1):47-54.
15. Braat AK, van de Water S, Goos H et al. Vasa protein expression and localization in the zebrafish. Mech Dev 2000;95(1-2):271-274.
16. Noce T, Okamoto-Ito S, Tsunekawa N. Vasa homolog genes in mammalian germ cell development. Cell Struct Funct 2001;26(3):131-136.
17. Toyooka Y, Tsunekawa N, Takahashi Y et al. Expression and intracellular localization of mouse Vasa-homologue protein during germ cell development. Mech Dev 2000;93(1-2):139-149.
18. Minematsu T, Harumi T, Naito M. Germ cell-specific expression of GFP gene induced by chicken vasa homologue (Cvh) promoter in early chicken embryos. Mol Reprod Dev 2008;75(10):1515-1522.
19. Kobayashi T, Yoshizaki G, Takeuchi Y et al. Isolation of highly pure and viable primordial germ cells from rainbow trout by GFP-dependent flow cytometry. Mol Reprod Dev 2004;67(1):91-100.
20. Seki Y, Yamaji M, Yabuta Y et al. Cellular dynamics associated with the genome-wide epigenetic reprogramming in migrating primordial germ cells in mice. Development 2007;134(14):2627-2638.
21. Stojkovic M, Lako M, Stojkovic P et al. Derivation of human embryonic stem cells from day-8 blastocysts recovered after three-step in vitro culture. Stem Cells 2004;22(5):790-797.
22. Rugg-Gunn PJ, Ferguson-Smith AC, Pedersen RA. Epigenetic status of human embryonic stem cells. Nat Genet 2005;37(6):585-587.
23. Dahl JA, Collas P. A quick and quantitative chromatin immunoprecipitation assay for small cell samples. Front Biosci 2007;12:4925-4931.
24. Dahl JA, Collas P. Q2ChIP, a quick and quantitative chromatin immunoprecipitation assay, unravels epigenetic dynamics of developmentally regulated genes in human carcinoma cells. Stem Cells 2007; 25(4):1037-1046.
25. King DC, Taylor J, Elnitski L et al. Evaluation of regulatory potential and conservation scores for detecting cis-regulatory modules in aligned mammalian genome sequences. Genome Res 2005;15(8): 1051-1060.
26. Anderson RA, Fulton N, Cowan G et al. Conserved and divergent patterns of expression of DAZL, VASA and OCT4 in the germ cells of the human fetal ovary and testis. BMC Dev Biol 2007;7:136.
27. Mise N, Fuchikami T, Sugimoto M et al. Differences and similarities in the developmental status of embryo-derived stem cells and primordial germ cells revealed by global expression profiling. Genes Cells 2008;13(8):863-877.
28. Chalmel F, Rolland AD, Niederhauser-Wiederkehr C et al. The conserved transcriptome in human and rodent male gametogenesis. Proc Natl Acad Sci U S A 2007;104(20):8346-8351.
29. Zheng P, Dean J. Oocyte-specific genes affect folliculogenesis, fertilization, and early development. Semin Reprod Med 2007;25(4): 243-251.
30. Toyoda S, Miyazaki T, Miyazaki S et al. Sohlh2 affects differentiation of KIT positive oocytes and spermatogonia. Dev Biol 2009;325(1): 238-248.
31. Hock R, Witte F, Brocher J et al. Expression of HMGA2 variants during oogenesis and early embryogenesis of Xenopus laevis. Eur J Cell Biol 2006;85(6):519-528.
32. Sleer LS, Taylor CC. Cell-type localization of platelet-derived growth factors and receptors in the postnatal rat ovary and follicle. Biol Reprod 2007;76(3):379-390.
33. Swain JE, Ding J, Wu J et al. Regulation of spindle and chromatin dynamics during early and late stages of oocyte maturation by aurora kinases. Mol Hum Reprod 2008;14(5):291-299.
34. Osterlund C, Töhönen V, Forslund KO et al. Mage-b4, a novel melanoma antigen (MAGE) gene specifically expressed during germ cell differentiation. Cancer Res 2000;60(4):1054-1061.
35. Dietrich AJ, Mulder RJ. A light- and electron microscopic analysis of meiotic prophase in female mice. Chromosoma 1983;88(5): 377-385.
36. Clark SJ, Harrison J, Paul CL et al. High sensitivity mapping of methylated cytosines. Nucleic Acids Res 1994;22(15):2990-2997.
37. Kawakami T, Zhang C, Okada Y et al. Erasure of methylation imprint at the promoter and CTCF-binding site upstream of H19 in human testicular germ cell tumors of adolescents indicate their fetal germ cell origin. Oncogene 2006;25(23):3225-3236.
38. Borghol N, Loronage J, Blachere T et al. Epigenetic status of the H19 locus in human oocytes following in vitro maturation. Genomics 2006;87(3):417-426.
39. Takai D, Gonzales FA, Tsai YC et al. Large scale mapping of methylcytosines in CTCF-binding sites in the human H19 promoter and aberrant hypomethylation in human bladder cancer. Hum Mol Genet 2001; 10(23):2619-2626.
40. Sasaki H, Matsui Y. Epigenetic events in mammalian germ-cell development: reprogramming and beyond. Nat Rev Genet 2008;9(2): 129-140.