Effects of vitamin C and n-acetylcysteine against cyclophosphamide-induced genotoxicity in exfoliated bladder cells of mice in vivo

Journal of B.U.ON.

Volumn 14, Issue 4, 2009, Pages 647-652

  Gurbuz, Neslihan ^a   Ozkul, A ^b   Burgaz, S ^a  

^a GAZI UNIVERSITY   (Turkey)

^b ANKARA UNIVERSITY   (Turkey)

Author keywords

Chemoprevention; Exfoliated bladder cells; Genotoxicity; Micronucleus; N acetylcysteine; Vitamin C

Indexed keywords

ACETYLCYSTEINE; ASCORBIC ACID; CYCLOPHOSPHAMIDE; SODIUM CHLORIDE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BLADDER BLEEDING; BLADDER EPITHELIUM; BLADDER MUCOSA; BLADDER WALL; CANCER PREVENTION; CELL INFILTRATION; CELL ISOLATION; CELL PROTECTION; CONTROLLED STUDY; CYSTITIS; DESQUAMATION; DOSE RESPONSE; DRUG CYTOTOXICITY; DRUG DOSE COMPARISON; EPITHELIUM CELL; FEULGEN STAINING; GENOTOXICITY; HISTOPATHOLOGY; MALE; MICRONUCLEUS TEST; MOUSE; NONHUMAN; TREATMENT DURATION;

ACETYLCYSTEINE; ANIMALS; ANTINEOPLASTIC AGENTS, ALKYLATING; ANTIOXIDANTS; ASCORBIC ACID; CYCLOPHOSPHAMIDE; HEMORRHAGE; INFLAMMATION; MALE; MICE; MICE, INBRED BALB C; MICRONUCLEI, CHROMOSOME-DEFECTIVE; MICRONUCLEUS TESTS; URINARY BLADDER;

EID: 75149141886     PISSN: 11070625     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (46)

1. Morse MA, Stoner GD. Cancer chemoprevention, principles and prospects. Carcinogenesis 1993; 14:1737-1746.
2. Stich HF, San RHC, Rosin MP. Adaptation of the DNA repair and micronucleus tests to human cell suspensions and exfoliated cells. Ann NY Acad Sci 1983; 407:93-105.
3. Rosin MP. The use of the micronucleus tests on exfoliated cells to identify anti-clastogenic action in humans; A biological marker for the efficacy of chemopreventive agents. Mutat Res 1992; 267:265-276.
4. Konopacka M. Evaluation of frequency of micronuclei in exfoliated cells from bladder of mice treated with benzo(a) pyrene, 2-acetylaminofloren and cyclophosphamide. Cell Biol Int 1994; 18:669-672.
5. Konopacka M, Widel M, Wolny JR. Modifying effect of vitamin C, E and beta-carotene against gamma ray-induced DNA damage in mouse cells. Mutat Res 1998; 417:85-94.
6. De Flora S, Cesarone CF, Balansky RM et al. Chemopreventive properties and mechanisms of N-acetylcysteine. The experimental background. J Cell Biochem 1995; (Suppl 22): 33-41.
7. Bonorden WR, Paria MW. Antioxidant nutrients and protection from free radicals. In: Kotsonis FN, Mackey M, Hjelle J (Eds): Nutritional Toxicology, Raven Press, New York, 1994, pp 19-47.
8. Vijayalaxmi KK, Venu R. In vivo anticlastogenic effects of L-ascorbic acid in mice. Mutat Res 1999; 438:47-51.
9. De Flora S, D'Agostini F, Izotti A, Balansky R. Prevention by N-acetylcysteine of benzo(a)pyrene clastogenicity and DNA adducts in rats. Mutat Res 1991; 250:87-93.
10. Countryman PJ, Heddle JA. The production of micronuclei from chromosome aberrations in irradiated cultures of human lymphocytes. Mutat Res 1976; 41:321-332.
11. Silliphant WM, Townsend FM, Ambrogi LP (Eds). Manual of histologic and special staining technics (2nd Edn). New York, Toronto, London: McGraw Hill Book Company Inc, 1960.
12. Ferguson LR. Antimutagens as cancer chemopreventive agents in the diet. Mutat Res 1994; 307:395-410.
13. Majer BJ, Laky B, Knasmüller S, Kassie F. Use of micronucleus assay with exfoliated epithelial cells as a biomarker for monitoring individuals at elevated risk of genetic damage and in chemoprevention trials. Mutat Res 2001: 489(2-3);147-72
14. Burgaz S, Cakmak G, Erdem O, Yilmaz M, Karakaya AE. Micronuclei frequencies in exfoliated nasal mucosa cells from pathology and anatomy laboratory workers exposed to formaldehyde. Neoplasma 2001; 48:144-147.
15. Burgaz S, Karahalil B, Bayrak P et al. Urinary cyclophosphamide excretion and micronuclei frequencies in peripheral lymphocytes and in exfoliated buccal epithelial cells of nurses handling antineoplastics. Mutat Res 1999; 439:97-104.
16. Burgaz S, Iscan A, Buyukbingol ZK, Bozkurt A, Karakaya AE. Evaluation of micronuclei in exfoliated urothelial cells and urinary thioether excretion of smokers. Mutat Res 1995; 335:163-169.
17. Nersesyan AK. Possible role of the micronucleus assay in diagnostics and secondary prevention of cervix cancer: a mini review. Tsitol Genet 2007; 41:64-66.
18. Chung FL, Xuy Ho CT, Desai D, Han C. Protection against tobacco-specific, nitrosamine-induced lung tumorigenesis by green tea and its components. In: Huang MT, Ho CT, Lee CT, Le CY (Eds): Phenolic compounds in food and their effects on health. Antioxidants and Cancer Prevention. Am Chem Society, Washington DC, 1992, pp 300-307.
19. Crook TR, Souhami RL, McLean AEM. Cytotoxicity, DNA crosslinking and single strand breaks induced by activated cyclophosphamide and acrolein in human leukemia cells. Cancer Res 1986; 46:5029-5034.
20. Sakata T, Smith RA, Garland, Cohen SM. Rat urinary bladder epithelial lesions induced by acrolein. J Environ Pathol Toxicol Oncol 1989; 9:159-169.
21. Salvadori DMF, Riberio LR, Oliveira MDM, Peneria CAB, Becak V. The protective effect of B-carotene on genotoxicity induced by cyclophosphamide Mutat Res 1992; 265: 237-244.
22. De Kristina A, Agarwal K, Mukherjec A, Sengupta D. Inhibition by capsaicin against cyclophosphamide-induced clastogenicity and DNA damage in mice. Mutat Res 1995; 335: 253-258.
23. Roberts JC, Francetic DJ. Chemoprotection against cyclophosphamide- induced urotoxicity. Anticancer Res 1994; 14: 383-388.
24. Chorvatovicova D, Navarova J. Suppressing effects of glucan on micronuclei induced by cyclophosphamide in mice. Mutat Res 1992; 282:147-150.
25. Chorvatovicova D, Bauer V. Stobadine-inhibitor of cyclophosphamide- induced micronuclei in mice. Mutagenesis 1994; 9:241-244.
26. Sen S, Mukherjee A, Agarwall K, Sharma A. Phenethyl isothiocyanate modulates clastogenicity of mitomycin C and cyclophosphamide in vivo. Mutat Res 1996; 371:159-164.
27. Nersesyan A, Muradyan R, Arsenyan F. The influence of three newly synthesized pyrimidine-containing compounds on micronucleus induction and tumor growth. J BUON 2007; 12: 521-527.
28. Bose S, Sinha SP. Modulation of ochratoxin-produced genotoxicity in mice by vitamin C. Food Chem Toxicol 1994; 32: 533-537.
29. Roberts JC, Francetic DJ. Chemoprotection against cyclophosphamide- induced urotoxicity. Ribose-Cysteine. Anti-cancer Res 1994; 14:383-388.
30. Reynolds JEF (Ed): Martindale, The Extra Pharmacopoeia (31st Edn). The Pharmaceutical Press, London, 1996, pp 427-455.
31. Amara-Mokrane YA, Lehucher-Michel MP, Balansard G, Dumenil G, Botta A. Protective effects of alpha-hederin, chlorophyllin and ascorbic acid towards the induction of micronuclei by doxorubicin in cultured human lymphocytes. Mutagenesis 1996; 11:161-167.
32. Siddique YH, Beg T, Afzal M, Antigenotoxic effects of ascorbic acid against megestrol acetate-induced genotoxicity in mice. Hum Exp Toxicol 2005; 24:121-127.
33. De Vires N, De Flora S. N-acetyl-1-cysteine. J Cell Biochem 1993 (Suppl 17F): 270-277.
34. De Flora S, Izotti A, Di Agostini F, Balansky RM. Mechanisms of N-acetylcysteine in the prevention of DNA damage and cancer, with special reference to smoking-related end-points. Carcinogenesis 2001; 22:999-1013.
35. De Flora, Izotti A, Di Agostini F, Cesarone CF. Antioxidant activity and other, mechanisms of thiols involved in chemoprevention of mutation and cancer. Am J Med 1991; 91 (Suppl 3c):122-130.
36. Boone CW, Steele VE, Kelloff GI. Screening for chemopreventive (anticarcinogenic) compounds in rodents. Mutat Res 1992; 267:251-255.
37. Wei M, Arnold L, Cano M et al. Effects of co-administration of antioxidants and arsenicals on the rat urinary bladder epithelium. Toxicol Sci 2004; 83:237-245.
38. Chen N, Aleksa K, Woodland C, Rieder M, Koren G. N-acetylcysteine prevents ifosfamide-induced nephrotoxicity in rats. Br J Pharmacol 2008; 153:1364-1372
39. Kaplan M, Atakan IH, Aydogdu N et al. Influence of N-acetylcysteine on renal toxicity of cadmium in rats. Pediatr Nephrol 2008; 23:233-241.
40. Izotti A, Orlando M, Gasparini L et al. In vitro inhibition by N-acetylcysteine of oxidative DNA modifications detected by 32 P postlabelling. Free Radie Res 1998; 28:165-178.
41. Zhao C, Shichi H. Prevention of acetaminophen-induced cataract by a combination of diallyl disulfide and N-acetylcysteine. J Ocul Pharmacol Ther 1998; 14:345-355.
42. Balansky RM, De Flora S. Chemoprevention by N-acetylcysteine of urethane-induced clastogenicity and lung tumors in mice. Int J Cancer 1998; 77:302-305.
43. Gurtoo HL, Hipkens JH, Sharma SD. Role of glutathione in the metabolism-depended toxicity and chemotherapy of cyclophosphamide. Cancer Res 1981; 41:3584-3591.
44. Berrigan MJ, Marinello AJ, Pavelic Z, Williams CJ, Struck RF, Gurto HL. Protective role of thiols in cyclophosphamideinduced urotoxicity and depression of hepatic drug metabolism. Cancer Res 1982; 42:3688-3695.
45. Di Agostini F, Balansky RM, Camoirano A, De Flora S. Interactions between N-acetylcysteine and ascorbic acid in modulating mutagenesis and carcinogenesis. Int J Cancer 2000; 88:702-707.
46. Balansky RM, De Flora S. Interactions between N-acetyl-cysteine and sodium selenite in modulating the clastogenicity of urethane and 2-acetylaminofluorene in mice. Int J Cancer 2004; 108:158-161.