Brain phosphoinositide extraction, fractionation, and analysis by MALDI-TOF MS

Methods in Molecular Biology

Volumn 579, Issue , 2009, Pages 189-200

  Johanson, Roy A ^a   Berry, Gerard T ^b  

^a THOMAS JEFFERSON UNIVERSITY   (United States)

^b BOSTON CHILDREN S HOSPITAL   (United States)

Author keywords

MALDI TOF MS; Phosphoinositides

Indexed keywords

PHOSPHATIDYLCHOLINE; PHOSPHATIDYLINOSITIDE; PHOSPHATIDYLINOSITOL; PHOSPHATIDYLINOSITOL 4 PHOSPHATE; PHOSPHATIDYLINOSITOL 4,5 BISPHOSPHATE; CATION EXCHANGE RESIN;

ADSORPTION; ANALYTIC METHOD; ARTICLE; BRAIN TISSUE; CATION EXCHANGE; EXTRACTION; FRACTIONATION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; SEPARATION TECHNIQUE; ANIMAL; BRAIN; C57BL MOUSE; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; MOUSE; STANDARD;

ANIMALS; BRAIN; CATION EXCHANGE RESINS; CHROMATOGRAPHY, ION EXCHANGE; MICE; MICE, INBRED C57BL; PHOSPHATIDYLINOSITOLS; REFERENCE STANDARDS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 74949092964     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60761-322-0_9     Document Type: Article

References (20)

1. Han, X. and Gross, R. W. (2005) Shotgun lipidomics: electrospray ionization mass spectrometric analysis and quantitation of cellular lipidomes directly from crude extracts of biological samples. Mass Spectrom. Rev. 24, 367–412.
2. Han, X. and Cheng, H. (2005) Characterization and direct quantitation of cerebroside molecular species from lipid extracts by shotgun lipidomics. J. Lipid Res. 46, 163–175.
3. Schiller, J., Suss, R., Arnhold, J., Fuchs, B., Lessig, J., Muller, M., Petkovic, M., Spalteholz, H., Zschornig, O., and Arnold, K. (2004) Matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass spectrometry in lipid and phospholipid research. Prog. Lipid Res. 43, 449–488.
4. Johanson, R. A., Buccafusca, R., Quong, J. N., Shaw, M. A., and Berry, G. T. (2007) Phosphatidylcholine removal from brain lipid extracts expands lipid detection and enhances phosphoinositide quantification by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Anal. Biochem. 362, 155–167.
5. Milne, S., Ivanova, P., Forrester, J., and Alex Brown, H. (2006) Lipidomics: an analysis of cellular lipids by ESI-MS. Methods 39, 92–103.
6. Petkovic, M., Schiller, J., Muller, M., Benard, S., Reichl, S., Arnold, K., and Arnhold, J. (2001) Detection of individual phospholipids in lipid mixtures by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry: phosphatidylcholine prevents the detection of further species. Anal. Biochem. 289, 202–216.
7. Schiller, J., Arnhold, J., Benard, S., Muller, M., Reichl, S., and Arnold, K. (1999) Lipid analysis by matrix-assisted laser desorption and ionization mass spectrometry: A methodological approach. Anal. Biochem. 267, 46–56.
8. Muller, M., Schiller, J., Petkovic, M., Oehrl, W., Heinze, R., Wetzker, R., Arnold, K., and Arnhold, J. (2001) Limits for the detection of (poly-)phosphoinositides by matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS). Chem. Phys. Lipids 110, 151–164.
9. Schiller, J., Suss, R., Fuchs, B., Muller, M., Zschornig, O., and Arnold, K. (2003) Combined application of TLC and matrixassisted laser desorption and ionization timeof- flight mass spectrometry (MALDI-TOF MS) to phospholipid analysis of brain. Chromatographia 57, S297–S302.
10. Schacht, J. (1981) Extraction and purification of polyphosphoinositides. Methods Enzymol. 72, 626–631.
11. Arthur, G. and Sheltawy, A. (1980) The presence of lysophosphatidylcholine in chromaffin granules. Biochem. J. 191, 523–532.
12. Christie, W. W. (1993) (Ed.), Advances in Lipid Methodology – Two, Oily Press, Dundee, 1993, pp. 195–213. (available online at http://www.lipidlibrary.co.uk/topics/extract2/ index.htm)
13. Hauser, G., Eichberg, J., and Gonzalez-Sastre, F. (1971) Regional distribution of polyphosphoinositides in rat brain. Biochim. Biophys. Acta 248, 87–95.
14. Hauser, G. and Eichberg, J. (1973) Improved conditions for the preservation and extraction of polyphosphoinositides. Biochim. Biophys. Acta 326, 201–209.
15. Nishihara, M. and Keenan, R. W. (1985) Inositol phospholipid levels of rat forebrain obtained by freeze-blowing method. Biochim. Biophys. Acta 835, 415–418.
16. Bligh, E. G. and Dyer, W. J. (1959) A rapid method for total lipid extraction and purification. Can. J. Biochem. Physiol. 37, 911–917.
17. Hendrickson, H. S. and Ballou, C. E. (1964) Ion exchange chromatography of intact brain phosphoinositides on diethylaminoethyl cellulose by gradient salt elution in a mixed solvent system. J. Biol. Chem. 239, 1369–1373.
18. Kiselev, G. V. (1982) Preparative isolation of polyphosphoinositide fractions from ox brain. Biochim. Biophys. Acta 712, 719–721.
19. Low, M. G. (1990) Purification of Phosphatidylinositol 4-Phosphate and Phosphatidylinositol 4,5-Bisphosphate by Column Chromatography. In: Methods in Inositide Research, (Irvine, R. F. ed.) Raven Press, New York, pp.145–151.
20. Baldwin, M. A. (2005) Mass spectrometers for the analysis of biomolecules. Methods Enzymol. 402, 3–48.