Identification, cloning and expression of a second gene (vpr1) from the venom of the endoparasitic wasp, Pimpla hypochondriaca that displays immunosuppressive activity

Journal of Insect Physiology

Volumn 56, Issue 2, 2010, Pages 195-203

  Dani, M Paulina ^a   Richards, Elaine H ^a  

^a FOOD AND ENVIRONMENT RESEARCH AGENCY   (United Kingdom)

Author keywords

Encapsulation; Endoparasitoid venom; Haemocyte anti aggregation; Pimpla hypochondriaca; Recombinant venom protein

Indexed keywords

INSECT PROTEIN; WASP VENOM;

AMINO ACID; BACTERIUM; ENDOPARASITE; GENE EXPRESSION; IMMUNE RESPONSE; PROTEIN; VENOM; WASP;

ANIMAL; ARTICLE; BLOOD CELL; CHEMISTRY; DRUG EFFECT; GENETICS; IMMUNOLOGY; ISOLATION AND PURIFICATION; MOLECULAR CLONING; MOLECULAR GENETICS; MOTH; PARASITOLOGY; WASP;

ANIMALS; CLONING, MOLECULAR; HEMOCYTES; INSECT PROTEINS; MOLECULAR SEQUENCE DATA; MOTHS; WASP VENOMS; WASPS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PIMPLA HYPOCHONDRIACA;

EID: 74849138637     PISSN: 00221910     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jinsphys.2009.10.006     Document Type: Article

References (39)

1. Asgari S., Zhang G., Zareie R., and Schmidt O. A serine proteinase homolog venom protein from an endoparasitoid wasp inhibits melanization of host haemolymph. Insect Biochem. Mol. Biol. 33 (2003) 1017-1024
2. Asgari S., Zareie R., Zhang G., and Schmidt O. Isolation and characterization of a novel venom protein from an endoparasitoid, Cotesia rubecula (Hym: Braconidae). Arch. Insect Biochem. Physiol. 53 (2003) 92-100
3. Beckage N.E. Modulation of immune responses to parasitoids by polydnaviruses. Parasitology 116 (1998) S57-S64
4. Cai J., Ye G.-Y., and Hu C. Parasitism of Pieris rapae (Lepidoptera: Pieridae) by a pupal endoparasitoid, Pteromalus puparum (Hymenoptera: Pteromalidae): effects of parasitization and venom on host hemocytes. J. Insect Physiol. 50 (2004) 315-322
5. Colinet D., Schmitz A., Depoix D., Crochard D., and Poirié M. Convergent use of RhoGAP toxins by eukaryotic parasites and bacterial pathogens. PLOS Pathogens 3 (2007) 2029-2037
6. Dani M.P., Richards E.H., Isaac R.E., and Edwards J.P. Antibacterial and proteolytic activity in venom from the endoparasitic wasp Pimpla hypochondriaca (Hymenoptera: Ichneumonidae). J. Insect Physiol. 49 (2003) 945-954
7. Dani M.P., Edwards J.P., and Richards E.H. Hydrolase activity in the venom of the pupal endoparasitic wasp Pimpla hypochondriaca. Comp. Biochem. Physiol. B: Biochem. Mol. Biol. 141 (2005) 373-381
8. Dani M.P., and Richards E.H. Cloning and expression of the gene for an insect haemocyte anti-aggregation protein (VPr3), from the venom of the endoparasitic wasp, Pimpla hypochondriaca. Arch. Insect Biochem. Physiol. 71 (2009) 191-204
9. Fritton M.G., Shaw M.R., and Gauld I.D. Pimpline Ichneumon-flies. In: Barnard P.C., and Askew R.R. (Eds). Handbooks for the Identification of British Insects vol. 7, Part 1 (1988), British Museum (Natural History), London
10. Gupta A.P. Insect immunocytes and other haemocytes: role in cellular and humeral immunity. In: Gupta A.P. (Ed). Immunology of Insects and Other Arthropods (1991), CRC Press, Boca Raton, FL 19-118
11. Kitano H. The role of Apanteles glomeratus venom in defensive response of its host, Pieris rapae crucivora. J. Insect Physiol. 32 (1986) 369-375
12. Krishnan A., Nair P.N., and Jones D. Isolation, cloning, and characterization of a new chitinase stored in active form in chitin-lined venom reservoir. J. Biol. Chem. 269 (1994) 20971-20976
13. Labrosse C., Staslak K., Lesobre J., Grangeia A., Huguet E., Drezen J.M., and Poirié M. A RhoGAP protein as a main immune suppressive factor in the Leptopilina boulardi (Hymenoptera, Figitidae)-Drosophila melanogaster interaction. Insect Biochem. Mol. Biol. 35 (2005) 93-103
14. Labrosse C., Eslin P., Doury G., Drezen J.M., and Poirié. Haemocytes changes in D. melangoaster in response to long gland components of the parasitoid wasp Leptopilina boulardi: a Rho-GAP protein as an important factor. J. Insect Physiol. 51 (2005) 161-170
15. Lackie A.M. Haemocyte behaviour. Adv. Insect Physiol. 21 (1988) 85-177
16. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 277 (1970) 680-685
17. Lavine M.D., and Beckage N.E. Polydnaviruses: potent mediators of host insect immune dysfunction. Parasitol. Today 11 (1995) 365-378
18. McCullagh P., and Nelder J.A. Generalized Linear Models (1992), Chapman and Hall, London
19. Novagen. pET System Manual. 11th ed. (2006)
20. Parkinson N.M., and Weaver R.J. Noxious components of venom from the pupa-specific parasitoid Pimpla hypochondriaca. J. Invertebr. Pathol. 73 (1999) 74-83
21. Parkinson N., Smith I., Weaver R., and Edwards J.P. A new form of arthropod phenoloxidase is abundant in venom of the parasitoid wasp Pimpla hypochondriaca. Insect Biochem. Mol. Biol. 31 (2001) 57-63
22. Parkinson N., Richards E.H., Conyers C., Smith I., and Edwards J.P. Analysis of venom constituents from the parasitoid wasp Pimpla hypochondriaca and cloning of a cDNA encoding a venom protein. Insect Biochem. Mol. Biol. 32 (2002) 729-735
23. Parkinson N., Conyers C., and Smith I. A venom protein from the endoparasitoid wasp Pimpla hypochondriaca is similar to snake venom reprolysin-type metalloproteases. J. Invertebr. Pathol. 79 (2002) 129-131
24. Parkinson N., Smith I., Audsley N., and Edwards J.P. Purification of pimplin, a paralytic heterodimeric polypeptide from venom of the parasitoid wasp Pimpla hypochondriaca, and cloning of the cDNA encoding one of the subunits. Insect Biochem. Mol. Biol. 32 (2002) 1769-1773
25. Parkinson N., Richards E.H., Conyers, Keen J.N., MacNicoll A.D.C., Smith I., and Weaver R.J. cDNAs encoding large venom proteins from the parasitoid wasp Pimpla hypochondriaca identified by random sequence analysis. Comp. Biochem. Physiol. Part C 134 (2003) 513-520
26. Richards E.H., and Parkinson N.M. Venom from the endoparasitic wasp Pimpla hypochondriaca adversely affects the morphology, viability and immune function of haemocytes from larvae of the tomato moth, Lacanobia oleracea. J. Invertebr. Pathol. 76 (2000) 33-42
27. Richards E.H., and Dani M.P. Biochemical isolation of an insect haemocyte anti-aggregation protein from the venom of the endoparasitic wasp. Pimpla hypochondriaca, and identification of its gene. J. Insect Physiol. 54 (2008) 1041-1049
28. Rowley A.F., and Ratcliffe N.A. Insects. In: Ratcliffe N.A., and Rowley A.F. (Eds). Invertebrate Blood Cells (1981), Academic Press, New York 471-490
29. Salt G. The resistance of insect parasitoids to the defence reactions of their hosts. Biol. Rev. 43 (1968) 200-232
30. Strand M.R., and Pech L.L. Immunological basis for compatibility in parasitoid-host relationships. Annu. Rev. Entomol. 40 (1995) 31-56
31. Tanaka T. Effect of the venom of the endoparasitoid, Apanteles kariyai Watanabe, on the cellular defense reaction of the host, Pseudeletia separata Walker. J. Insect Physiol. 33 (1987) 413-420
32. Taylor T., and Jones D. Isolation and characterization of the 32.5 kDa protein from the venom of an endoparasitic wasp. Biochim. et Biophys. Acta 1035 (1990) 37-43
33. Thompson W.R. Part 8: parasites of Lepidoptera. In: Section 1: Parasite Host Catalogue. Catalogue of the Parasites and Predators of Insect Pests (1946) pp. 479-481
34. Thompson W.R. Part 4. Hosts of Hymenoptera. In: Section 2: Host parasite catalogue. A Catalogue of the Parasites and Predators of Insect Pests (1957) pp. 519-520
35. Vinson S.B. How parasitoids deal with the immune system of their host: an overview. Arch. Insect Biochem. Physiol. 13 (1990) 3-27
36. Vinson S.B., and Iwantsch G.F. Host regulation by insect parasitoids. Q. Rev. Biol. 55 (1980) 143-165
37. Wu M.-L., Ye G.-Y., Zhu J.-Y., Chen X.-X., and Cui H. Isolation and characterization of an immunosuppressive protein from venom of the pupa-specific endoparasitoid Pteromalus puparum. J. Invertebr. Pathol. 99 (2008) 186-191
38. Zhang G., Schmidt O., and Asgari S. A novel peptide from an endoparasitoid wasp is required for expression of polydnavirus gene in host hemocytes. J. Biol. Chem. 279 (2004) 41580-41585
39. Zhang G., Schmidt O., and Asgari S. A calreticulin-like protein from endoparasitoid venom fluid is involved in host haemocyte inactivation. Dev. Comp. Immunol. 30 (2006) 756-764