Isolation and characterization of an immunosuppressive protein from venom of the pupa-specific endoparasitoid Pteromalus puparum

Journal of Invertebrate Pathology

Volumn 99, Issue 2, 2008, Pages 186-191

  Wu, Ma li ^a   Ye, Gong yin ^a   Zhu, Jia ying ^a   Chen, Xue xin ^a   Hu, Cui ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

Chromatography; Encapsulation; Hemocyte spreading; Immune suppression; Pieris rapae; Pteromalus puparum; Venom

Indexed keywords

IMMUNOSUPPRESSIVE AGENT; WASP VENOM;

ANIMAL; ARTICLE; BLOOD CELL; BUTTERFLY; CHEMISTRY; DRUG EFFECT; IMMUNOLOGY; INSECT; POLYACRYLAMIDE GEL ELECTROPHORESIS; WASP;

ANIMALS; BUTTERFLIES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HEMOCYTES; IMMUNOSUPPRESSIVE AGENTS; PUPA; WASP VENOMS; WASPS;

HEXAPODA; PIERIS RAPAE; PTEROMALUS PUPARUM;

EID: 52049090112     PISSN: 00222011     EISSN: 10960805     Source Type: Journal    
DOI: 10.1016/j.jip.2008.07.005     Document Type: Article

References (51)

1. Abt M., and Rivers D.B. Characterization of phenoloxidase activity in venom from the ectoparasitoid Nasonia vitripennis (Walker) (Hyemnoptera: Pteromalidae). J. Invertebr. Pathol. 94 (2007) 108-118
2. Asgari S. Venom proteins from polydnavirus-producing endoparasitoids: their role in host-parasite interactions. Arch. Insect Biochem. Physiol. 61 (2006) 146-156
3. Asgari S. Endoparasitoid venom proteins as modulators of host immunity and development. In: Rivers D., and Yolder J. (Eds). Recent Advances in the Biochemistry, Toxicity, and Mode of Action of Parasitic Wasp Venoms (2007), Research Signpost, Kerala, India 57-73
4. Asgari S., Zareie R., Zhang G., and Schmidt O. Isolation and characterization of a novel venom protein from an endoparasitoid, Cotesia rubecula (Hym. Braconidae). Arch. Insect Biochem. Physiol. 53 (2003) 92-100
5. Asgari S., Zhang G., Zareie R., and Schmidt O. A serine proteinase homolog venom protein from an endoparasitoid wasp inhibits melanization of the host hemolymph, Insect Biochem. Mol. Biol. 33 (2003) 1017-1024
6. Beckage N.E., and Gelman D.B. Wasp parasitoid disruption of host development: implications for new biologically based strategies for insect control. Annu. Rev. Entomol. 49 (2004) 299-330
7. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
8. Cai J., Ye G.Y., and Hu C. Parasitism of Pieris rapae (Lepidoptera: Pieridae) by a pupal endoparasitoid, Pteromalus puparum (Hymenoptera: Pteromalidae): effects of parasitization and venom on host hemocytes. J. Insect Physiol. 50 (2004) 315-322
9. Dani M.P., Edwards J.P., and Richards E.H. Hydrolase activity in the venom of the pupal endoparasitic wasp, Pimpla hypochondriaca. Comp. Biochem. Physiol. 141B (2005) 373-381
10. Dani M.P., Richards E.H., Isaac R.E., and Edwards J.P. Antibacterial and proteolytic activity in venom from the endoparasitic wasp Pimpla hypochondriaca (Hymenoptera: Ichneumonidae). J. Insect Physiol. 49 (2003) 945-954
11. Digilio M.C., Isidoro N., Tremblay E., and Pennacchio F. Host castration by Aphidius ervi venom proteins. J. Insect Physiol. 46 (2000) 1041-1050
12. Ergin E., Uçkan F., and Rivers D.B. Biochemical characterization and mode of action of venom from the endoparasitoid wasp Pimpla turionellae L. In: Rivers D., and Yolder J. (Eds). Recent Advances in the Biochemistry, Toxicity, and Mode of Action of Parasitic Wasp Venoms (2007), Research Signpost, Kerala, India 129-160
13. Falabella P., Riviello L., Caccialupi P., Rossodivita T., Valente M.T., De Stradis M.L., Tranfaglia A., Varricchio P., Gigliotti S., Graziani F., Malva C., and Pennacchio F. A γ-glutamyl transpeptidase of Aphidius ervi venom induces apoptosis in the ovaries of host aphids. Insect Biochem. Mol. Biol. 37 (2007) 453-465
14. Glatz R.V., Asgari S., and Schmidt O. Evolution of polydnaviruses as insect immune suppressors. Trends Microbiol. 12 (2004) 545-554
15. Jones D., Sawicki G., and Wozniak M. Sequence, structure and expression of a wasp venom protein with a negatively charged signal peptide and a novel repeating internal structure. J. Biol. Chem. 267 (1992) 14871-14878
16. Krishnan A., Nair P.N., and Jones D. Isolation, cloning, and characterization of new chitinase stored in active from in chitin-linked venom reservoir. J. Biol. Chem. 269 (1994) 20971-20976
17. Labrosse C., Eslin P., Doury G., Drezen J.M., and Poirié M. Haemocyte changes in D. melanogaster in response to long gland components of the parasitoid wasp Leptopilina boulardi: a Rho-GAP protein as an important factor. J. Insect Physiol. 51 (2005) 161-170
18. Labrosse C., Stasiak K., Lesobre J., Grangeia A., Huguet E., Drezen J.M., and Poirié M. A RhoGAP protein as a main immune suppressive factor in the Leptopilina boulard (Hymenoptera, Figitidae)-Drosophila melanogaster interaction. Insect Biochem. Mol. Biol. 35 (2005) 93-103
19. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
20. Moreau S.J.M., and Guillot S. Advances and prospects on biosynthesis, structures and functions of venom proteins from parasitic wasps. Insect Biochem. Mol. Biol. 35 (2005) 1209-1223
21. Moreau S.J.M., Cherqui A., Dubois F., Doury G., Fourdrain Y., Bulet P., Sabatier L., Saarela J., Prévost G., and Giordanengo P. Identification of an aspartyglucosaminidase-like protein in the venom pf the parasitic wasp Asobara tabida (Hymenoptera: Braconidae). Insect Biochem. Mol. Biol. 34 (2004) 485-492
22. Nakamatsu Y., and Tanaka T. Venom of ectoparasitoid, Euplectrus sp. near plathypenae (Hymenoptera: Eulophidae) regulates the physiological state of Pseudaletia separate (Lepidoptera: Noctuidae) host as food resource. J. Insect Physiol. 49 (2003) 149-159
23. Osman S.E. Die wirkung der secrete der weiblichen genitalanhangsdrüsen von Pimpla turionellae L. (Hym., Ichneumonidae) auf die hämocyten und die einkapselungsreaktion von wirtspuppen. Z. Parasitenk. 57 (1978) 89-100
24. Parkinson N.M., and Weaver R.J. Noxious components of venom from the pupa-specific parasitoid Pimpla hypochondriaca. J. Invertebr. Pathol. 73 (1999) 74-83
25. Parkinson N., Smith I., Weaver R., and Edwards J.P. A new form of arthropod phenoloxidase is abundant in venom of the parasitoid wasp Pimpla hypochondriaca. Insect Biochem. Mol. Biol. 31 (2001) 57-63
26. Parkinson N., Richards E.H., Conyers C., Smith I., and Edwards J.P. Analysis of venom constituents from the parasitoid wasp Pimpla hypochondriaca and cloning of a cDNA encoding a venom protein. Insect Biochem. Mol. Biol. 32 (2002) 729-735
27. Parkinson N., Smith I., Audsley N., and Edwards J.P. Purification of pimplin, a paralytic heterodimeric polypeptide from venom of the parasitoid wasp Pimpla hypochondriaca and cloning of the cDNA encoding one of the subunits. Insect Biochem. Mol. Biol. 32 (2002) 1769-1773
28. Parkinson N.M., Conyers C., and Smith I. A venom protein from the endoparasitoid wasp Pimpla hypochondriaca is similar to snake venom reprolysin-type metalloproteases. J. Invertebr. Pathol. 79 (2002) 129-131
29. Parkinson N.M., Conyers C.M., Keen J.N., MacNicoll A.D., Smith I., and Weaver R.J. cDNAs encoding large venom proteins from the parasitoid wasp Pimpla hypochondriaca identified by random sequence analysis. Comp. Biochem. Physiol. 134C (2003) 513-520
30. Parkinson N.M., Conyers C.M., Keen J.N., MacNicoll A.D., Smith I., Audsley N., and Weaver R.J. Towards a comprehensive view of the primary structure of venom proteins from the paracitoid wasp Pimpla hypochondriaca. Insect Biochem. Mol. Biol. 34 (2004) 565-571
31. Richards E.H., and Parkinson N.M. Venom from the endoparasitic wasp Pimpla hypochondriaca adversely affects the morphology, viability, and immune function of hemocytes from larvae of the tomato moth, Lacanobia oleracea. J. Invertebr. Pathol. 76 (2000) 33-42
32. Rivers D.B., Uckan F., and Ergin E. Characterization and biochemical analyses of venom from the ectoparasitic wasp Nasonia vitripennis (Walker) (Hymenoptera: Pteromalidae). Arch. Insect. Biochem. Physiol. 61 (2006) 21-41
33. Rivers D.B., Hink W.F., and Denlinger D.L. Toxicity of the venom from Nasonia vitripennis (Hymenoptera: Pteromalidae) towards fly hosts, nontarget insects, different developmental stages, and cultured insect cell. Toxicon 31 (1993) 755-765
34. Rivers D.B., Ruggiero L., and Hayes M. The ectoparasitic wasp Nasonia vitripennis (Walker) (Hymenoptera: Pteromalidae) differentially affects cells mediating the immune response of its flesh fly host, Sarcophaga bullata Park (Diptera: Sarcophagidae). J. Insect Physiol. 48 (2002) 1053-1064
35. Schmidt O., Theopold U., and Strand M. Innate immunity and its evasion and suppression by hymenopteran endoparasitoids. BioEssays 23 (2001) 344-351
36. Shelby K.S., and Webb B.A. Polydnavirus-mediated suppression of insect immunity. J. Insect Physiol. 45 (1999) 507-514
37. Simpson R.J. Purifying Proteins for Proteomics: A Laboratory Manual (2004), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York pp. 713-718
38. Strand M.R., and Pech L.L. Immunological basis for compatibility in parasitoid-host relationships. Annu. Rev. Entomol. 40 (1995) 31-56
39. Swain M., and Ross N.W. A silver stain protocol for proteins yielding high resolution and transparent background in sodium dodecyl sulfate-polyacrylamide gels. Electrophoresis 16 (1995) 948-951
40. © Data Processing System: Experimental Design, Statistical Analysis Data Mining (http://www.chinadps.net). Science Press, Beijing, China.
41. Uçkan F., Sinan S., Savaşçi S., and Ergin E. Determination of Venom Components from the Endoparasitoid Wasp Pimpla turionellae L. (Hymenoptera: Ichneumonidae). Ann. Entomol. Soc. Am. 97 (2004) 775-780
42. Vass E., and Nappi A.J. Developmental and immunological aspects of Drosophila-parasitoid relationships. J. Parasitol. 86 (2000) 1259-1270
43. Vinson S.B. How parasitoids deal with the immune system of their host: an overview. Arch. Insect Biochem. Physiol. 13 (1990) 3-27
44. Ye G.Y., Zhu J.Y., Zhang Z., Fang Q., Cai J., and Hu C. Venom from the endoparasitoid Pteromalus puparum (Hymenoptera: Pteromalidae) adversely affects host hemocytes: differential toxicity and microstructural and ultrastructural changes in plasmatocytes and granular cells. In: Rivers D., and Yolder J. (Eds). Recent Advances in the Biochemistry, Toxicity, and Mode of Action of Parasitic Wasp Venoms (2007), Research Signpost, Kerala, India 115-127
45. Zhang G., Schmidt O., and Asgari S. A calreticulin-like protein from endoparasitoid venom fluid is involved in host hemocyte inactivation. Dev. Comp. Immunol. 30 (2006) 756-764
46. Zhang G., Schmidt O., and Asgari S. A novel venom peptide from an endoparasitoid wasp is required for expression of polydnavirus genes in host hemocytes. J. Biol. Chem. 279 (2004) 41580-41585
47. Zhang G., Lu Z.Q., Jiang H., and Asgari S. Negative regulation of prophenoloxidase (proPO) activation by a clip-domain serine proteinase homolog (SPH) from endoparasitoid venom. Insect Biochem. Mol. Biol. 34 (2004) 477-483
48. Zhang Z., Ye G.Y., and Hu C. Effects of venom from two pteromalid wasps Pteromalus puparum and Nasonia vitripennis (Hymenoptera: Pteromalidae) on the spreading, viability and encapsulation capacity of Pieris rapae hemocytes. Acta Entomol. Sinica 47 (2004) 551-561
49. Zhang Z., Ye G.Y., Cai J., and Hu C. Comparative venom toxicity between Pteromalus puparum and Nasonia vitripennis (Hymenoptera: Pteromalidae) toward the hemocytes of their natural hosts, non-target insects and cultured insect cells. Toxicon 46 (2005) 337-349
50. Zhu J.Y., Ye G.Y., and Hu C. Molecular cloning and characterization of acid phosphatase in venom of the endoparasitoid wasp Pteromalus puparum (Hymenoptera: Pteromalidae). Toxicon 51 (2008) 1391-1399
51. Zhu J.Y., Ye G.Y., and Hu C. Morphology and ultrastructure of the venom apparatus in the endoparasitic wasp, Pteromalus puparum (Hymenoptera: Pteromalidae). Micron 39 (2007) 926-933