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Volumn 170, Issue 2, 2010, Pages 84-92

Biochemical and biophysical characterisation of DBL1α1-varO, the rosetting domain of PfEMP1 from the VarO line of Plasmodium falciparum

Author keywords

Oligosaccharide binding; PfEMP1; Plasmodium falciparum; Recombinant domains; Rosetting

Indexed keywords

DUFFY BINDING LIKE DOMAIN ALPHA PROTEIN; DUFFY BINDING PROTEIN; ERYTHROCYTE MEMBRANE PROTEIN 1; HEPARIN; MALTOSE BINDING PROTEIN; OLIGOSACCHARIDE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

EID: 74849134917     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2009.12.008     Document Type: Article
Times cited : (26)

References (44)
  • 2
    • 0029052741 scopus 로고
    • Cloning the P. falciparum gene encoding PfEMP1, a malarial variant antigen and adherence receptor on the surface of parasitized human erythrocytes
    • Baruch D.I., Pasloske B.L., Singh H.B., et al. Cloning the P. falciparum gene encoding PfEMP1, a malarial variant antigen and adherence receptor on the surface of parasitized human erythrocytes. Cell 82 (1995) 77-87
    • (1995) Cell , vol.82 , pp. 77-87
    • Baruch, D.I.1    Pasloske, B.L.2    Singh, H.B.3
  • 3
    • 0029070914 scopus 로고
    • The large diverse gene family var encodes proteins involved in cytoadherence and antigenic variation of Plasmodium falciparum-infected erythrocytes
    • Su X.Z., Heatwole V.M., Wertheimer S.P., et al. The large diverse gene family var encodes proteins involved in cytoadherence and antigenic variation of Plasmodium falciparum-infected erythrocytes. Cell 82 (1995) 89-100
    • (1995) Cell , vol.82 , pp. 89-100
    • Su, X.Z.1    Heatwole, V.M.2    Wertheimer, S.P.3
  • 4
    • 33746318909 scopus 로고    scopus 로고
    • A family affair: var genes, PfEMP1 binding, and malaria disease
    • Kraemer S.M., and Smith J.D. A family affair: var genes, PfEMP1 binding, and malaria disease. Curr Opin Microbiol 9 (2006) 374-380
    • (2006) Curr Opin Microbiol , vol.9 , pp. 374-380
    • Kraemer, S.M.1    Smith, J.D.2
  • 5
    • 0033727467 scopus 로고    scopus 로고
    • Classification of adhesive domains in the Plasmodium falciparum erythrocyte membrane protein 1 family
    • Smith J.D., Subramanian G., Gamain B., Baruch D.I., and Miller L.H. Classification of adhesive domains in the Plasmodium falciparum erythrocyte membrane protein 1 family. Mol Biochem Parasitol 110 (2000) 293-310
    • (2000) Mol Biochem Parasitol , vol.110 , pp. 293-310
    • Smith, J.D.1    Subramanian, G.2    Gamain, B.3    Baruch, D.I.4    Miller, L.H.5
  • 6
    • 0035521149 scopus 로고    scopus 로고
    • Decoding the language of var genes and Plasmodium falciparum sequestration
    • Smith J.D., Gamain B., Baruch D.I., and Kyes S. Decoding the language of var genes and Plasmodium falciparum sequestration. Trends Parasitol 17 (2001) 538-545
    • (2001) Trends Parasitol , vol.17 , pp. 538-545
    • Smith, J.D.1    Gamain, B.2    Baruch, D.I.3    Kyes, S.4
  • 7
    • 0037015614 scopus 로고    scopus 로고
    • Genome sequence of the human malaria parasite Plasmodium falciparum
    • Gardner M.J., Hall N., Fung E., et al. Genome sequence of the human malaria parasite Plasmodium falciparum. Nature 419 (2002) 498-511
    • (2002) Nature , vol.419 , pp. 498-511
    • Gardner, M.J.1    Hall, N.2    Fung, E.3
  • 8
    • 0032530397 scopus 로고    scopus 로고
    • Antigenic variation in malaria: in situ switching, relaxed and mutually exclusive transcription of var genes during intra-erythrocytic development in Plasmodium falciparum
    • Scherf A., Hernandez-Rivas R., Buffet P., et al. Antigenic variation in malaria: in situ switching, relaxed and mutually exclusive transcription of var genes during intra-erythrocytic development in Plasmodium falciparum. EMBO J 17 (1998) 5418-5426
    • (1998) EMBO J , vol.17 , pp. 5418-5426
    • Scherf, A.1    Hernandez-Rivas, R.2    Buffet, P.3
  • 9
    • 0032560859 scopus 로고    scopus 로고
    • Developmental selection of var gene expression in Plasmodium falciparum
    • Chen Q., Fernandez V., Sundström A., et al. Developmental selection of var gene expression in Plasmodium falciparum. Nature 394 (1998) 392-395
    • (1998) Nature , vol.394 , pp. 392-395
    • Chen, Q.1    Fernandez, V.2    Sundström, A.3
  • 10
    • 0025612532 scopus 로고
    • Human cerebral malaria: association with erythrocyte rosetting and lack of anti-rosetting antibodies
    • Carlson J., Helmby H., Hill A.V., Brewster D., Greenwood B.M., and Wahlgren M. Human cerebral malaria: association with erythrocyte rosetting and lack of anti-rosetting antibodies. Lancet 336 (1990) 1457-1460
    • (1990) Lancet , vol.336 , pp. 1457-1460
    • Carlson, J.1    Helmby, H.2    Hill, A.V.3    Brewster, D.4    Greenwood, B.M.5    Wahlgren, M.6
  • 11
    • 0034701409 scopus 로고    scopus 로고
    • Autoagglutination of malaria-infected red blood cells and malaria severity
    • Roberts D.J., Pain A., Kai O., Kortok M., and Marsh K. Autoagglutination of malaria-infected red blood cells and malaria severity. Lancet 355 (2000) 1427-1428
    • (2000) Lancet , vol.355 , pp. 1427-1428
    • Roberts, D.J.1    Pain, A.2    Kai, O.3    Kortok, M.4    Marsh, K.5
  • 12
    • 42649140849 scopus 로고    scopus 로고
    • Rosetting is associated with increased Plasmodium falciparum in vivo multiplication rate in the Saimiri sciureus monkey
    • Le Scanf C., Vigan-Womas I., Contamin H., Guillotte M., Bischoff E., and Mercereau-Puijalon O. Rosetting is associated with increased Plasmodium falciparum in vivo multiplication rate in the Saimiri sciureus monkey. Microb Infect 10 (2008) 447-451
    • (2008) Microb Infect , vol.10 , pp. 447-451
    • Le Scanf, C.1    Vigan-Womas, I.2    Contamin, H.3    Guillotte, M.4    Bischoff, E.5    Mercereau-Puijalon, O.6
  • 13
    • 0031963302 scopus 로고    scopus 로고
    • Identification of Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) as the rosetting ligand of the malaria parasite P. falciparum
    • Chen Q., Barragan A., Fernandez V., et al. Identification of Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) as the rosetting ligand of the malaria parasite P. falciparum. J Exp Med 187 (1998) 15-23
    • (1998) J Exp Med , vol.187 , pp. 15-23
    • Chen, Q.1    Barragan, A.2    Fernandez, V.3
  • 14
    • 1842403514 scopus 로고    scopus 로고
    • P. falciparum rosetting mediated by a parasite-variant erythrocyte membrane protein and complement-receptor 1
    • Rowe J.A., Moulds J.M., Newbold C.I., and Miller L.H. P. falciparum rosetting mediated by a parasite-variant erythrocyte membrane protein and complement-receptor 1. Nature 388 (1997) 292-295
    • (1997) Nature , vol.388 , pp. 292-295
    • Rowe, J.A.1    Moulds, J.M.2    Newbold, C.I.3    Miller, L.H.4
  • 15
    • 57349087682 scopus 로고    scopus 로고
    • An in vivo and in vitro model of Plasmodium falciparum rosetting and autoagglutination mediated by varO, a group A var gene encoding a frequent serotype
    • Vigan-Womas, Guillotte M., Le Scanf C., et al. An in vivo and in vitro model of Plasmodium falciparum rosetting and autoagglutination mediated by varO, a group A var gene encoding a frequent serotype. Infect Immun 76 (2008) 5565-5580
    • (2008) Infect Immun , vol.76 , pp. 5565-5580
    • Vigan-Womas1    Guillotte, M.2    Le Scanf, C.3
  • 16
    • 0026770547 scopus 로고
    • Disruption of Plasmodium falciparum erythrocyte rosettes by standard heparin and heparin devoid of anticoagulant activity
    • Carlson J., Ekre H.P., Helmby H., Gysin J., Greenwood B.M., and Wahlgren M. Disruption of Plasmodium falciparum erythrocyte rosettes by standard heparin and heparin devoid of anticoagulant activity. Am J Trop Med Hyg 46 (1992) 595-602
    • (1992) Am J Trop Med Hyg , vol.46 , pp. 595-602
    • Carlson, J.1    Ekre, H.P.2    Helmby, H.3    Gysin, J.4    Greenwood, B.M.5    Wahlgren, M.6
  • 17
    • 0034103535 scopus 로고    scopus 로고
    • Blood group A antigen is a coreceptor in Plasmodium falciparum rosetting
    • Barragan A., Kremsner P.G., Wahlgren M., and Carlson J. Blood group A antigen is a coreceptor in Plasmodium falciparum rosetting. Infect Immun 68 (2000) 2971-2975
    • (2000) Infect Immun , vol.68 , pp. 2971-2975
    • Barragan, A.1    Kremsner, P.G.2    Wahlgren, M.3    Carlson, J.4
  • 18
    • 0037443518 scopus 로고    scopus 로고
    • Heparan sulfate on endothelial cells mediates the binding of Plasmodium falciparum-infected erythrocytes via the DBL1α domain of PfEMP1
    • Vogt A.M., Barragan A., Chen Q., Kironde F., Spillmann D., and Wahlgren M. Heparan sulfate on endothelial cells mediates the binding of Plasmodium falciparum-infected erythrocytes via the DBL1α domain of PfEMP1. Blood 101 (2003) 2405-2411
    • (2003) Blood , vol.101 , pp. 2405-2411
    • Vogt, A.M.1    Barragan, A.2    Chen, Q.3    Kironde, F.4    Spillmann, D.5    Wahlgren, M.6
  • 19
    • 0034548046 scopus 로고    scopus 로고
    • Mapping of the region of complement receptor (CR) 1 required for Plasmodium falciparum rosetting and demonstration of the importance of CR1 in rosetting in field isolates
    • Rowe J.A., Rogerson S.J., Raza A., et al. Mapping of the region of complement receptor (CR) 1 required for Plasmodium falciparum rosetting and demonstration of the importance of CR1 in rosetting in field isolates. J Immunol 165 (2000) 6341-6346
    • (2000) J Immunol , vol.165 , pp. 6341-6346
    • Rowe, J.A.1    Rogerson, S.J.2    Raza, A.3
  • 20
    • 0028004529 scopus 로고
    • Sulfated glycoconjugates as disrupters of Plasmodium falciparum erythrocyte rosettes
    • Rogerson S.J., Reeder J.C., Al-Yaman F., and Brown G.V. Sulfated glycoconjugates as disrupters of Plasmodium falciparum erythrocyte rosettes. Am J Trop Med Hyg 51 (1994) 198-203
    • (1994) Am J Trop Med Hyg , vol.51 , pp. 198-203
    • Rogerson, S.J.1    Reeder, J.C.2    Al-Yaman, F.3    Brown, G.V.4
  • 21
    • 0028566589 scopus 로고
    • Plasmodium falciparum: a family of sulphated glycoconjugates disrupts erythrocyte rosettes
    • Rowe A., Berendt A.R., Marsh K., and Newbold C.I. Plasmodium falciparum: a family of sulphated glycoconjugates disrupts erythrocyte rosettes. Exp Parasitol 79 (1994) 506-516
    • (1994) Exp Parasitol , vol.79 , pp. 506-516
    • Rowe, A.1    Berendt, A.R.2    Marsh, K.3    Newbold, C.I.4
  • 22
    • 41649094162 scopus 로고    scopus 로고
    • Disruption of rosetting in Plasmodium falciparum malaria with chemically modified heparin and low molecular weight derivatives possessing reduced anticoagulant and other serine protease inhibition activities
    • Skidmore M.A., Dumax-Vorzet A.F., Guimond S.E., et al. Disruption of rosetting in Plasmodium falciparum malaria with chemically modified heparin and low molecular weight derivatives possessing reduced anticoagulant and other serine protease inhibition activities. J Med Chem 510 (2000) 1453-1458
    • (2000) J Med Chem , vol.510 , pp. 1453-1458
    • Skidmore, M.A.1    Dumax-Vorzet, A.F.2    Guimond, S.E.3
  • 23
    • 0033562629 scopus 로고    scopus 로고
    • Analyzing protein circular dichroism spectra for accurate secondary structures
    • Johnson W.C. Analyzing protein circular dichroism spectra for accurate secondary structures. Proteins 35 (1999) 307-312
    • (1999) Proteins , vol.35 , pp. 307-312
    • Johnson, W.C.1
  • 24
    • 0036154258 scopus 로고    scopus 로고
    • Size-distribution analysis of proteins by analytical ultracentrifugation: strategies and application to model systems
    • Schuck P., Perugini M.S., Gonzales N.R., Howlett G.J., and Schubert D. Size-distribution analysis of proteins by analytical ultracentrifugation: strategies and application to model systems. Biophys J 82 (2002) 1096-1111
    • (2002) Biophys J , vol.82 , pp. 1096-1111
    • Schuck, P.1    Perugini, M.S.2    Gonzales, N.R.3    Howlett, G.J.4    Schubert, D.5
  • 25
    • 40449129266 scopus 로고    scopus 로고
    • Overproduction, purification and crystallization of a chondroitin sulfate A-binding DBL domain from a Plasmodium falciparum var2csa-encoded PfEMP1 protein
    • Higgins M.K. Overproduction, purification and crystallization of a chondroitin sulfate A-binding DBL domain from a Plasmodium falciparum var2csa-encoded PfEMP1 protein. Acta Crystallogr F 64 (2008) 221-223
    • (2008) Acta Crystallogr F , vol.64 , pp. 221-223
    • Higgins, M.K.1
  • 26
    • 0001229341 scopus 로고    scopus 로고
    • Calculation of hydrodynamic properties of globular proteins from their atomic-level structure
    • Garcia de la Torre J., Huertas M.L., and Carrasco B. Calculation of hydrodynamic properties of globular proteins from their atomic-level structure. Biophys J 78 (2000) 719-730
    • (2000) Biophys J , vol.78 , pp. 719-730
    • Garcia de la Torre, J.1    Huertas, M.L.2    Carrasco, B.3
  • 27
    • 51349112071 scopus 로고    scopus 로고
    • The structure of a chondroitin sulfate-binding domain important in placental malaria
    • Higgins M.K. The structure of a chondroitin sulfate-binding domain important in placental malaria. J Biol Chem 283 (2008) 21842-21846
    • (2008) J Biol Chem , vol.283 , pp. 21842-21846
    • Higgins, M.K.1
  • 28
    • 0028899619 scopus 로고
    • Immune pressure selects for Plasmodium falciparum parasites presenting distinct red blood cell surface antigens and inducing strain-specific protection in Saimiri sciureus monkeys
    • Fandeur T., Le Scanf C., Bonnemains B., Slomianny C., and Mercereau-Puijalon O. Immune pressure selects for Plasmodium falciparum parasites presenting distinct red blood cell surface antigens and inducing strain-specific protection in Saimiri sciureus monkeys. J Exp Med 181 (1995) 283-295
    • (1995) J Exp Med , vol.181 , pp. 283-295
    • Fandeur, T.1    Le Scanf, C.2    Bonnemains, B.3    Slomianny, C.4    Mercereau-Puijalon, O.5
  • 29
    • 0031088904 scopus 로고    scopus 로고
    • A modification in restriction pattern of the Plasmodium falciparum Pf60 multigene family associated with a specific antigenic variation switch in the Palo Alto line
    • Le Scanf C., Carcy B., Bonnefoy S., Fandeur T., and Mercereau-Puijalon O. A modification in restriction pattern of the Plasmodium falciparum Pf60 multigene family associated with a specific antigenic variation switch in the Palo Alto line. Behring Inst Mitt 99 (1997) p16-p24
    • (1997) Behring Inst Mitt , Issue.99
    • Le Scanf, C.1    Carcy, B.2    Bonnefoy, S.3    Fandeur, T.4    Mercereau-Puijalon, O.5
  • 30
    • 0027291015 scopus 로고
    • Prediction of protein secondary structure at better than 70% accuracy
    • Rost B., and Sander C. Prediction of protein secondary structure at better than 70% accuracy. J Mol Biol 232 (1993) 584-599
    • (1993) J Mol Biol , vol.232 , pp. 584-599
    • Rost, B.1    Sander, C.2
  • 31
    • 22744456158 scopus 로고    scopus 로고
    • Structural basis for the EBA-175 erythrocyte invasion pathway of the malaria parasite Plasmodium falciparum
    • Tolia N.H., Enemark E.J., Sim B.K., and Joshua-Tor L. Structural basis for the EBA-175 erythrocyte invasion pathway of the malaria parasite Plasmodium falciparum. Cell 122 (2005) 183-193
    • (2005) Cell , vol.122 , pp. 183-193
    • Tolia, N.H.1    Enemark, E.J.2    Sim, B.K.3    Joshua-Tor, L.4
  • 32
    • 33845432121 scopus 로고    scopus 로고
    • Receptorbinding studies of the DBLγ domain of Plasmodium falciparum erythrocyte membrane protein 1 from a placental isolate
    • Badaut C., Faure G., Tuikue Ndam N.G., et al. Receptorbinding studies of the DBLγ domain of Plasmodium falciparum erythrocyte membrane protein 1 from a placental isolate. Mol Biochem Parasitol 151 (2007) 89-99
    • (2007) Mol Biochem Parasitol , vol.151 , pp. 89-99
    • Badaut, C.1    Faure, G.2    Tuikue Ndam, N.G.3
  • 33
    • 32544440337 scopus 로고    scopus 로고
    • Structural basis for Duffy recognition by the malaria parasite Duffybinding-like domain
    • Singh S.K., Hora R., Belrhali H., Chitnis C.E., and Sharma A. Structural basis for Duffy recognition by the malaria parasite Duffybinding-like domain. Nature 439 (2006) 741-744
    • (2006) Nature , vol.439 , pp. 741-744
    • Singh, S.K.1    Hora, R.2    Belrhali, H.3    Chitnis, C.E.4    Sharma, A.5
  • 34
    • 51349147433 scopus 로고    scopus 로고
    • Structure of the DBL3x domain of pregnancy-associated malaria protein VAR2CSA complexed with chondroitin sulfate A
    • Singh K., Gittis A.G., Nguyen P., Gowda D.C., Miller L.H., and Garboczi D.N. Structure of the DBL3x domain of pregnancy-associated malaria protein VAR2CSA complexed with chondroitin sulfate A. Nat Struct Mol Biol 15 (2008) 932-938
    • (2008) Nat Struct Mol Biol , vol.15 , pp. 932-938
    • Singh, K.1    Gittis, A.G.2    Nguyen, P.3    Gowda, D.C.4    Miller, L.H.5    Garboczi, D.N.6
  • 35
    • 0042326726 scopus 로고    scopus 로고
    • Definition of structural elements in Plasmodium vivax and P. knowlesi Duffy-binding domains necessary for erythrocyte invasion
    • Singh S.K., Singh A.P., Pandey S., Yazandi S.S., Chitnis C.E., and Amit Shama A. Definition of structural elements in Plasmodium vivax and P. knowlesi Duffy-binding domains necessary for erythrocyte invasion. Biochem J 374 (2003) 193-198
    • (2003) Biochem J , vol.374 , pp. 193-198
    • Singh, S.K.1    Singh, A.P.2    Pandey, S.3    Yazandi, S.S.4    Chitnis, C.E.5    Amit Shama, A.6
  • 36
    • 68349128076 scopus 로고    scopus 로고
    • Optimizing expression of the pregnancy malaria vaccine candidate, VAR2CSA in Pichia pastoris
    • Avril M., Hathaway M.J., Cartwright M.M., Gose S.O., Narum D.L., and Smith J.D. Optimizing expression of the pregnancy malaria vaccine candidate, VAR2CSA in Pichia pastoris. Malar J 8 (2009) 143
    • (2009) Malar J , vol.8 , pp. 143
    • Avril, M.1    Hathaway, M.J.2    Cartwright, M.M.3    Gose, S.O.4    Narum, D.L.5    Smith, J.D.6
  • 38
    • 59849124093 scopus 로고    scopus 로고
    • Role of the Plasmodium export element in trafficking parasite proteins to the infected erythrocyte
    • Boddey J.A., Moritz R.L., Simpson R.J., and Cowman A.F. Role of the Plasmodium export element in trafficking parasite proteins to the infected erythrocyte. Traffic c10 (2009) 285-299
    • (2009) Traffic , vol.c10 , pp. 285-299
    • Boddey, J.A.1    Moritz, R.L.2    Simpson, R.J.3    Cowman, A.F.4
  • 39
    • 67650609882 scopus 로고    scopus 로고
    • Chondroitin sulphate A (CSA)-binding of single recombinant Duffy-binding-like domains is not restricted to Plasmodium falciparum erythrocyte membrane protein 1 expressed by CSA-binding parasites
    • Resende M., Ditlev S.B., Nielsen M.A., et al. Chondroitin sulphate A (CSA)-binding of single recombinant Duffy-binding-like domains is not restricted to Plasmodium falciparum erythrocyte membrane protein 1 expressed by CSA-binding parasites. Int J Parasitol 39 (2009) 1195-1204
    • (2009) Int J Parasitol , vol.39 , pp. 1195-1204
    • Resende, M.1    Ditlev, S.B.2    Nielsen, M.A.3
  • 40
    • 0035907331 scopus 로고    scopus 로고
    • Biochemical, biophysical, and functional characterization of bacterially expressed and refolded receptor binding domain of Plasmodium vivax duffy-binding protein
    • Singh S., Pandey K., Chattopadhayay R., et al. Biochemical, biophysical, and functional characterization of bacterially expressed and refolded receptor binding domain of Plasmodium vivax duffy-binding protein. J Biol Chem 276 (2001) 17111-17116
    • (2001) J Biol Chem , vol.276 , pp. 17111-17116
    • Singh, S.1    Pandey, K.2    Chattopadhayay, R.3
  • 41
    • 0037197772 scopus 로고    scopus 로고
    • Antibodies raised against receptor-binding domain of Plasmodium knowlesi Duffy binding protein inhibit erythrocyte invasion
    • Singh A.P., Puri S.K., and Chitnis C.E. Antibodies raised against receptor-binding domain of Plasmodium knowlesi Duffy binding protein inhibit erythrocyte invasion. Mol Biochem Parasitol 121 (2002) 21-31
    • (2002) Mol Biochem Parasitol , vol.121 , pp. 21-31
    • Singh, A.P.1    Puri, S.K.2    Chitnis, C.E.3
  • 42
    • 38049050264 scopus 로고    scopus 로고
    • Plasmodium vivax invasion of human erythrocytes inhibited by antibodies directed against the Duffy binding protein
    • Grimberg B.T., Udomsangpetch R., Xainli J., et al. Plasmodium vivax invasion of human erythrocytes inhibited by antibodies directed against the Duffy binding protein. PLoS Med 4 (2007) e337
    • (2007) PLoS Med , vol.4
    • Grimberg, B.T.1    Udomsangpetch, R.2    Xainli, J.3
  • 43
    • 69049084603 scopus 로고    scopus 로고
    • Functional and immunological characterization of a Duffy binding-like α domain from Plasmodium falciparum erythrocyte membrane protein 1 that mediates rosetting
    • Mayor A., Rovira-Vallbona E., Srivastava A., et al. Functional and immunological characterization of a Duffy binding-like α domain from Plasmodium falciparum erythrocyte membrane protein 1 that mediates rosetting. Infect Immun 77 (2009) 3857-3863
    • (2009) Infect Immun , vol.77 , pp. 3857-3863
    • Mayor, A.1    Rovira-Vallbona, E.2    Srivastava, A.3
  • 44
    • 60749136552 scopus 로고    scopus 로고
    • A general method for the selection of high-level scFv and IgG antibody expression by stably transfected mammalian cells
    • Zuberbühler K., Palumbo A., Bacci C., et al. A general method for the selection of high-level scFv and IgG antibody expression by stably transfected mammalian cells. Protein Eng Des Sel 22 (2009) 169-174
    • (2009) Protein Eng Des Sel , vol.22 , pp. 169-174
    • Zuberbühler, K.1    Palumbo, A.2    Bacci, C.3


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