Technical note: Efficiency of total demineralization and ion-exchange column for DNA extraction from bone

American Journal of Physical Anthropology

Volumn 141, Issue 1, 2010, Pages 158-162

  Seo, Seung Bum ^a   Zhang, Aihua ^a   Kim, Hye Yeon ^a   Yi, Jin A ^a   Lee, Hye Young ^a   Shin, Dong Hoon ^a   Lee, Soong Deok ^a  

^a Seoul National University   (South Korea)

Author keywords

Bone; DNA extraction; Ion exchange column; Total demineralization

Indexed keywords

ARTICLE; BONE DEMINERALIZATION; CONTROLLED STUDY; DNA EXTRACTION; DNA PURIFICATION; GENE LOCUS; HUMAN; ION EXCHANGE; ION EXCHANGE CHROMATOGRAPHY; POSTHUMOUS CARE; REAL TIME POLYMERASE CHAIN REACTION;

BONE AND BONES; BONE DEMINERALIZATION TECHNIQUE; CHROMATOGRAPHY, ION EXCHANGE; DNA; FORENSIC ANTHROPOLOGY; HUMANS; POLYMERASE CHAIN REACTION;

EID: 74749099380     PISSN: 00029483     EISSN: 10968644     Source Type: Journal    
DOI: 10.1002/ajpa.21193     Document Type: Article

References (22)

1. Adachi N, Shinoda K, Umetsu K, Matsumura H. 2009. Mitochondrial DNA analysis of Jomon skeletons from the Funadomari site. Hokkaido, and its implication for the origins of Native American. Am J Phys Anthropol 138:255-265.
2. Applied Biosystems. 2005. Quantifiler™ human DNA quantification kit user's manual. Foster City (CA): Applied Biosystems.
3. Applied Biosystems. 2006. AmpFlSTR® Identifiler® PCR amplification kit user's manual. Foster City (CA): Applied Biosystems.
4. Braid MD, Daniels LM, Kitts CL. 2003. Removal of PCR inhibitors from soil DNA by chemical flocculation. J Microbiol Methods 52:389-393.
5. Davoren J, Vanek D, Konjhodzić R, Crews J, Huffine E, Parsons TJ. 2007. Highly effective DNA extraction method for nuclear short tandem repeat testing of skeletal remains from mass graves. Croat Med J 48:478-485.
6. Gill P, Ivanov PL, Kimpton C, Piercy R, Benson N, Tully G, Evett I, Hagelberg E, Sullivan K. 1994. Identification of the remains of the Romanov family by DNA analysis. Nat Genet 6:130-135.
7. Holmlund G, Lodestad I, Nilsson H, Lindblom B. 2006. Experiences from DNA analysis in Sweden for the identification of tsunami victims. Int Congr Ser 1288:744-746.
8. Kemp BM, Malhi RS, McDonough J, Bolnick DA, Eshleman JA, Rickards O, Martinez-Labarga C, Johnson JR, Lorenz JG, Dixon EJ, Fifield TE, Heaton TH, Worl R, Smith DG. 2007. Genetic analysis of early holocene skeletal remains from Alaska and its implications for the settlement of the Americas. Am J Phys Anthropol 132:605-621.
9. Kemp BM, Smith DG. 2005. Use of bleach to eliminate contaminating DNA from the surface of bones and teeth. Forensic Sci Int 154:53-61.
10. Kim K, Kim KY, Jeon E, Togloom A, Cho YO, Lee MS, Lkhagvasuren G, Choi JH, Tumen D, Ja Park A, Kim KC, Park KW,
11. Kim JH, Noh M, Yoo KJ, Lee KH. 2008. Technical note: improved ancient DNA purification for PCR using ionexchange columns. Am J Phys Anthropol 136:114-121.
12. Kurosaki K, Matsushita T, Ueda S. 1993. Individual DNA identification from ancient human remains. Am J Hum Genet 53:638-643.
13. Lindahl T. 1993. Instability and decay of the primary structure of DNA. Nature 362:709-715.
14. Loreille OM, Diegoli TM, Irwin JA, Coble MD, Parsons TJ. 2007. High efficiency DNA extraction from bone by total demineralization. Forensic Sci Int Genet 1:191-195.
15. Nasidze I, Quinque D, Dupanloup I, Cordaux R, Kokshunova L, Stoneking M. 2005. Genetic evidence for the Mongolian ancestry of Kalmyks. Am J Phys Anthropol 128:846-854.
16. Poinar HN. 2002. The genetic secrets some fossils hold. Acc Chem Res 35:676-684.
17. QIAGEN companies. 2001. QIAGEN Genomic DNA handbook.
18. QIAGEN companies. 2006. QIAquick® spin handbook.
19. Rohland N, Hofreiter M. 2007. Comparison and optimization of ancient DNA extraction. Biotechniques 42:343-352.
20. Stone AC, Milner GR, Pääbo S, Stoneking M. 1996. Sex determination of ancient human skeletons using DNA. Am J Phys Anthropol 99:231-238.
21. Tsai YL, Olson BH. 1992. Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction. Appl Environ Microbiol 58:2292-2295.
22. Yang DY, Eng B, Waye JS, Dudar JC, Saunders SR. 1998. Technical note: improved DNA extraction from ancient bones using silica-based spin columns. Am J Phys Anthropol 105:539-543.