Maintenance of human embryonic stem cells on gelatin

Chinese Science Bulletin

Volumn 54, Issue 22, 2009, Pages 4214-4220

  Li, Yang ^a   Lin, ChangSheng ^a   Wang, Li ^a   Liu, Ying ^a,b   Mu, XiaoNing ^c   Ma, Yue ^c   Li, LingSong ^a  

^a PEKING UNIVERSITY   (China)

^b CAPITAL MEDICAL UNIVERSITY   (China)

^c INSTITUTE OF BIOPHYSICS   (China)

Author keywords

Feeder free; Gelatin; Human embryonic stem cells; Matrigel; Pluripotency

Indexed keywords

EID: 74549219416     PISSN: 10016538     EISSN: 18619541     Source Type: Journal    
DOI: 10.1007/s11434-009-0630-4     Document Type: Article

References (15)

1. Reubinoff B E, Pera M F, Fong C Y, et al. Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro. Nat Biotechnol, 2000, 18: 399-404.
2. Thomson J A, Itskovitz-Eldor J, Shapiro S S, et al. Embryonic stem cell lines derived from human blastocysts. Science, 1998, 282: 1145-1147.
3. Bongso A. RMaFCY. From human embryos to clinically compliant embryonic stem cells: Blastocyst culture, xeno-free derivation and cryopreservation, properties and applications of embryonic stem cells. Singapore: World Scientific Publishing Co. Pte. Ltd., 2005.
4. Rosler E S, Fisk G J, Ares X, et al. Long-term culture of human embryonic stem cells in feeder-free conditions. Dev Dyn, 2004, 229: 259-274.
5. Amit M, Shariki C, Margulets V, et al. Feeder layer- and serum-free culture of human embryonic stem cells. Biol Reprod, 2004, 70: 837-845.
6. Sato N, Meijer L, Skaltsounis L, et al. Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor. Nat Med, 2004, 10: 55-63.
7. Xu R H, Peck R M, Li D S, et al. Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cells. Nat Methods, 2005, 2: 185-190.
8. Brimble S N, Zeng X, Weiler D A, et al. Karyotypic stability, genotyping, differentiation, feeder-free maintenance, and gene expression sampling in three human embryonic stem cell lines derived prior to August 9, 2001. Stem Cells Dev, 2004, 13: 585-597.
9. Beattie G M, Lopez A D, Bucay N, et al. Activin A maintains pluripotency of human embryonic stem cells in the absence of feeder layers. Stem Cells, 2005, 23: 489-495.
10. Klimanskaya I, Chung Y, Meisner L, et al. Human embryonic stem cells derived without feeder cells. Lancet, 2005, 365: 1636-1641.
11. Xu C, Inokuma M S, Denham J, et al. Feeder-free growth of undifferentiated human embryonic stem cells. Nat Biotechnol, 2001, 19: 971-974.
12. Stojkovic P, Lako M, Przyborski S, et al. Human-serum matrix supports undifferentiated growth of human embryonic stem cells. Stem Cells, 2005, 23: 895-902.
13. Yim E K, Leong K W. Proliferation and differentiation of human embryonic germ cell derivatives in bioactive polymeric fibrous scaffold. J Biomater Sci Polym Ed, 2005, 16: 1193-1217.
14. Li Y J, Chung E H, Rodriguez R T, et al. Hydrogels as artificial matrices for human embryonic stem cell self-renewal. J Biomed Mater Res A, 2006, 79: 1-5.
15. Solter D, Knowles B B. Immunosurgery of mouse blastocyst. Proc Natl Acad Sci USA, 1975, 72: 5099-5102.