Diagnosis of Neospora caninum in bovine fetuses by histology, immunohistochemistry, and nested-PCR;Pesquisa de Neospora caninum em fetos bovinos por histologia, imunoistoquimica e nested-PCR

Revista Brasileira de Parasitologia Veterinaria

Volumn 18, Issue 4, 2009, Pages 14-19

  Cabral, Aline Diniz ^a   Camargo, Clarice Neves ^a   Galleti, Nara Thiers Cacciatori ^a   Okuda, Liria Hiromi ^a   Pituco, Edviges Maristela ^a   del Fava, Claudia ^a  

^a Centro de Pesquisa e Desenvolvimento de Sanidade Animal CPDSA   (Brazil)

Author keywords

Abortion; Brazil; Cattle; Diagnosis; Neosporosis

Indexed keywords

ANIMALIA; BOS; BOVINAE; NEOSPORA CANINUM;

EID: 74349110915     PISSN: 0103846X     EISSN: None     Source Type: Journal    
DOI: 10.4322/rbpv.01804003     Document Type: Article

References (42)

1. ANDERSON, M. L. et al. Neospora-like protozoan infection as a major cause of abortion in California dairy cattle. Journal of the American Veterinary Medical Association, v. 198, n. 2, p. 241-244, 1991.
2. BARRATT, J. et al. The development and evaluation of a nested PCR assay for detection of Neospora caninum and Hammondia heydorni in feral mouse tissues. Molecular and Cellular Probes, v. 22, n. 4, p. 228-233, 2008.
3. BASZLER, T. V. et al. Detection by PCR of Neospora caninum in foetal tissues from spontaneous bovine abortions. Journal of Clinical Microbiology, v. 37, n. 12, p. 4059-4064, 1999.
4. BJERKAS, I.; MOHN, S. F.; PRESTHUS, J. Unidentified cyst-forming sporozoon causing encephalomyelitis and myositis in dogs. Zeithschrifft Fuer Parasitenkunde, v. 70, n. 2, p. 271-274, 1984.
5. BOENISCH, T. Effect of heat-induced antigen retrieval following inconsistent formalin fixation. Applied Immunohistochemistry & Molecular Morphology, v. 13, n. 3, p. 283-286, 2005.
6. Collantes-Fernandez, E. et al. Quantitative detection of Neospora caninum in bovine aborted fetuses and experimentally infected mice by real-time PCR. Journal of Clinical Microbiology, v. 40, n. 4, p. 1194-1198, 2002.
7. CORBELLINI, L. G. et al. Neosporosis as a cause of abortion in dairy cattle in Rio Grande do Sul, southern Brazil. Veterinary Parasitology, v. 103, n. 3, p. 195-202, 2002.
8. DUBEY, J. P. Review of Neospora caninum and neosporosis in animals. The Korean Journal of Parasitology, v. 41, n. 1, p. 1-16, 2003.
9. DUBEY, J. P. et al. Newly recognized fatal protozoan disease of dogs. Journal of the American Veterinary Medical Association, v. 192, n. 9, p. 1269-285, 1988.
10. DUBEY, J. P.; LINDSAY, D. S. A review of Neospora caninum and neosporosis. Veterinary Parasitology, v. 67, n. 1, p. 1-59, 1996.
11. DUBEY, J. P.; LINDSAY, D. S.; SPEER, C. A. Structures of Toxoplasma gondii tachyzoites, bradyzoites and sporozoites and biology and development of tissue cysts. Clinical Microbiology Reviews, v. 11, n. 2, p. 267-299, 1998
12. DUBEY, J. P.; SCHARES, G.; Ortega-Mora, L. M. Epidemiology and control of neosporosis and Neospora caninum. Journal of Clinical Microbiology, v. 20, n. 2, p. 323-367, 2007.
13. EPERON, S. et al. Susceptibility of B-cell deficient C57BL/6 (microMT) mice to Neospora caninum infection. Parasite Immunology, v. 21, n. 5, p. 225-36, 1999.
14. GONDIM, L. F. P. et al. Neospora caninum infection in an aborted bovine foetus in Brazil. New Zealand Veterinary Journal, v. 47, n. 1, p. 35, 1999.
15. GONDIM, L. F. P. et al. Variation of the internal transcribed spacer 1 sequence within individual strains and among different strains of Neospora caninum. Journal of Parasitology, v. 90, n. 1, p. 119-122, 2004b.
16. GONDIM, L. F. P. et al. Coyotes (Canis latrans) are definitive hosts of Neospora caninum. International Journal for Parasitology, v. 34, n. 2, p. 159-161, 2004a.
17. GOTTSTEIN, B. et al. Molecular and immunodiagnostic investigations on bovine neosporosis in Switzerland. International Journal for Parasitology, v. 28, n. 4, p. 679-691, 1998.
18. HOLMDAHL, O. J. M.; MATTSSON, J. G. Rapid and sensitive identification of Neospora caninum by in vitro amplification of the internal transcribed spacer 1. Parasitology, v. 112, n. 2, p. 177-182, 1996.
19. HUGHES, M. J. et al. The prevalence of Neospora caninum and coinfection with Toxoplasma gondii by PCR analysis in naturally occurring mammal populations. Parasitology, v. 132, n. 1, p. 29-36, 2006.
20. KEY, M.; BOENISCH, T. Antigen Retrieval. In: KEY, M. (ed.). Immunohistochemical staining methods. 4 ed. Carpinteria: Dako Corporation, 2006. p. 41-45.
21. KIRKBRIDE, C. A. Laboratory diagnosis of livestock abortion. 3 ed. Iowa State: University Press, 1990. 260 p.
22. LINDSAY, D. S.; DUBEY, J. P. Immunohistochemical diagnosis of Neospora caninum in tissue sections. American Journal of Veterinary Research, v. 50, n. 11, p. 1981-1983, 1989.
23. McAllister, M. M. et al. Dogs are definitive hosts of Neospora caninum. International Journal for Parasitology, v. 28, n. 9, p. 1473-1478, 1998.
24. MEDINA, L. et al. Survey of Neospora caninum infection by nested PCR in aborted fetuses from dairy farms in Aguascalientes, Mexico. Veterinary Parasitology, v. 136, n. 3-4, p. 187-191, 2006.
25. McNamee, P. T. et al. The diagnosis and prevalence of neosporosis in Northern Ireland cattle. The Veterinary Record, v. 138, n. 17, p. 419-420, 1996.
26. MONTEIRO, R. M. et al. Molecular phylogenetic analysis in Hammondia-like organisms based on partial Hsp70 coding sequences. Parasitology, v. 134, n. 9, p. 1195-1203, 2007.
27. MOORE, D. P. et al. The role of Neospora caninum and Toxoplasma gondii in spontaneous bovine abortion in Argentina. Veterinary Parasitology, v. 156, n. 3-4, p. 163-167, 2008.
28. OTTER, A. et al. A survey of the incidence of Neospora caninum infection in aborted and stillborn bovine fetuses in England and Wales. The Veterinary Record, v. 136, n. 24, p. 602-606, 1995.
29. PAULA, V. S. O. et al. Evaluation of a PCR based on primers to Nc5 gene for the detection of Neospora caninum in brain tissues of bovine aborted fetuses. Veterinary Research Communications, v. 28, n. 7, p. 581-585, 2004.
30. PAYNE, S.; ELLIS, J. Detection of Neospora caninum DNA by the polymerase chain reaction. International Journal for Parasitology, v. 26, n. 4, p. 347-351, 1996.
31. Pereira-Bueno, J. et al. Evaluation by different diagnostic techniques of bovine abortion associated with Neospora caninum in Spain. Veterinary Parasitology, v. 111, n. 2-3, p. 143-152, 2003.
32. PESCADOR, C. A. et al. Histopathological and immunohistochemical aspects of Neospora caninum diagnosis in bovine aborted fetuses. Veterinary Parasitology, v. 150, n. 1-2, p. 159-163, 2007.
33. Ramos-Vara, J. A. et al. Suggested guidelines for immunohistochemical techniques in veterinary diagnostic laboratories. Journal of Veterinary Diagnostic Investigation, v. 20, n. 4, p. 393-413, 2008.
34. SAGER, H. et al. A Swiss case-control study to assess Neospora caninumassociated bovine abortions by PCR, histopathology and serology. Veterinary Parasitology, v. 102, n. 1-2, p. 1-15, 2001.
35. SANTOS, A. P. M. E. et al. Neospora caninum in dairy cattle in Parana State, Brazil: histological and immunohistochemical analysis in fetuses. Semina: Ciências Agrárias, v. 26, n. 4, p. 559-562, 2005.
36. Schares, G. et al. The efficiency of vertical transmission of Neospora caninum in dairy cattle analysed by serological techniques. Veterinary Parasitology, v. 80, n. 2, p. 87-98, 1998.
37. SHIBATA, D. Preparation of nucleic acids from archival material. In: MULLIS, K. B.; FERRE, F.; GIBBS, R. A. (Eds.). The polymerase chain reaction. Boston: Birkhauser, 1994. p. 47-54.
38. Sondgen, P. et al. Bovine neosporosis: immunoblot improves foetal serology. Veterinary Parasitology, v. 102, n. 4, p. 279-290, 2001.
39. THILSTEAD, J. P.; DUBEY, J. P. Neosporosis-like abortions in a herd of dairy cattle. Journal of Veterinary Diagnostic Investigation, v. 1, n. 3, p. 205-209, 1989.
40. WIN EPISCOPE, 2000. Disponivel em: http://www.clive.ed.ac.uk/winepiscope/
41. WOUDA, W. et al. Bovine fetal neosporosis: a comparison of epizootic and sporadic abortion cases and different age classes with regard to lesion severity and immunohistochemical identification of organisms in brain, heart, and liver. Journal of Veterinary Diagnostic Investigation, v. 9, n. 2, p. 180-185, 1997.
42. YAMAGE, M.; FLECHTNER, O.; GOTTSTEIN, B. Neospora caninum: specific oligonucleotide primers for the detection of brain "cyst" DNA of experimentally infected nude mice by the polymerase chain reaction (PCR). Journal of Parasitology, v. 82, n. 2, p. 272-279, 1996.