A review of Neospora caninum and neosporosis

Veterinary Parasitology

Volumn 67, Issue 1-2, 1996, Pages 1-59

  Dubey, J P ^a   Lindsay, D S ^b  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

^b AUBURN UNIVERSITY   (United States)

Author keywords

abortion; animals; Neospora caninum; neosporosis; paralysis; tachyzoites; tissue cysts

Indexed keywords

CLINDAMYCIN; COTRIMAZINE; PYRIMETHAMINE; SULFADIAZINE; SULFONAMIDE; TRIMABAC; TRIMETHOPRIM; UNCLASSIFIED DRUG;

ABORTION; CATTLE; DRUG EFFICACY; INFECTION CONTROL; LIFE CYCLE; NONHUMAN; PARASITOSIS; PROTOZOAL INFECTION; REVIEW; TOXOPLASMA GONDII; VETERINARY MEDICINE;

ABORTION, VETERINARY; ANIMALS; CATTLE; CATTLE DISEASES; COCCIDIOSIS; DISEASE TRANSMISSION, VERTICAL; DOG DISEASES; DOGS; FEMALE; HUMANS; MOLECULAR SEQUENCE DATA; NEOSPORA; POLYMERASE CHAIN REACTION; PREGNANCY; PREGNANCY COMPLICATIONS, PARASITIC; ZOONOSES;

EID: 0030566651     PISSN: 03044017     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0304-4017(96)01035-7     Document Type: Review

References (193)

1. Abbitt, B., Craig, T.M., Jones, L.P., Huey, R.L. and Eugster, K., 1993. Protozoal abortion in a herd of cattle concurrently infected with Hammondia pardalis. J. Am. Vet. Med. Assoc., 3: 444-448.
2. Agerholm, J.S. and Barr, B.C., 1994. Bovine abortions associated with Neospora in Denmark. Acta Vet. Scand., 35: 461-464.
3. Anderson, M.L., Blanchard, P.C., Barr, B.C., Dubey, J.P., Hoffman, R.L. and Conrad, P.A., 1991. Neospora-like protozoan infection as a major cause of abortion in California dairy cattle. J. Am. Vet. Med. Assoc., 198: 241-244.
4. Anderson, M., Picanso, J., Thurmond, M., Blanchard, P., Layton, B., Palmer, C., Case, J., Barr, B., Dubey, J.P. and Conrad, P., 1992. Epidemiological investigations of bovine protozoal abortion in California dairy herds. In: E.I. Williams (Editor). Proceedings of the XVII World Buiatrics Congress and XXV American Association of Bovine Practitioners Conference, St. Paul, Minnesota. August 31-September 4, 1992. Stillwater, OK, Frontier Printers, Vol. 2: 74-78.
5. Anderson, M.L., Barr, B.C. and Conrad, P.A., 1994. Protozoal causes of reproductive failure in domestic ruminants. Vet. Clin. N. Am. Food Anim. Pract., 10: 439-461.
6. Anderson, M.L., Palmer, C.W., Thurmond, M.C., Picanso, J.P., Blanchard, P.C., Breitmeyer, R.E., Layton, A.W., McAllister, M., Daft, B., Kinde, H., Read, D.H., Dubey, J.P., Conrad, P.A. and Barr, B.C., 1995. Evaluation of abortions in cattle attributable to neosporosis in selected dairy herds in California. J. Am. Vet. Med. Assoc., 207: 1206-1210.
7. Baker, D.G., Morishita, T.Y., Brooks, D.L., Shen, S.K., Lindsay, D.S. and Dubey, J.P., 1995. Experimental oral inoculations in birds to evaluate potential definitive host of Neospora caninum. J. Parasitol., 81: 783-785.
8. Barber, J., Trees, A.J., Owen, M. and Tennant, B., 1993. Isolation of Neospora caninum from a British dog. Vet. Rec., 133: 531-532.
9. Barber, J.S., Holmdahl, O.J.M., Owen, M.R., Guy, F., Uggla, A. and Trees, A.J., 1995. Characterization of the first European isolate of Neospora caninum (Dubey, Carpenter, Speer, Topper and Uggla). Parasitology, 111: 563-568.
10. Barber, J.S. and Trees, A.J., 1996. Clinical aspects of twenty-seven cases of neosporosis in dogs. Vet. Rec., in press.
11. Barber, J.S., Payne-Johnson, C.E. and Trees, A.J., 1996. Distribution of Neospora caninum within the central nervous system and other tissues of six dogs with clinical neosporosis. J. Small Anim. Pract., in press.
12. Barr, B.C., Anderson, M.L., Blanchard, P.C., Daft, B.M., Kinde, H. and Conrad, P.A., 1990. Bovine fetal encephalitis and myocarditis associated with protozoal infections. Vet. Pathol., 27: 354-361.
13. Barr, B.C., Anderson, M.L., Dubey, J.P. and Conrad, P.A., 1991a. Neospora-like protozoal infections associated with bovine abortions. Vet. Pathol., 28: 110-116.
14. Barr, B.C., Conrad, P.A., Dubey, J.P. and Anderson, M.L., 1991b. Neospora-like encephalomyelitis in a calf: pathology, ultrastructure, and immunoreactivity. J. Vet. Diagn. Invest., 3: 39-46.
15. Barr, B.C., Anderson, M.L., Woods, L.W., Dubey, J.P. and Conrad, P.A., 1992. Neospora-like protozoal infections associated with abortion in goats. J. Vet. Diagn. Invest., 4: 365-367.
16. Barr, B.C., Conrad, P.A., Breitmeyer, R., Sverlow, K., Anderson, M.L., Reynolds, J., Chauvet, A.E., Dubey, J.P. and Ardans, A.A., 1993. Congenital Neospora infection in calves born from cows that had previously aborted Neospora-infected fetuses: Four cases (1990-1992). J. Am. Vet. Med. Assoc., 202: 113-117.
17. Barr, B.C., Conrad, P.A., Sverlow, K.W., Tarantal, A.F. and Hendrickx, A.G., 1994a. Experimental fetal and transplacental Neospora infection in the nonhuman primate. Lab. Invest., 71: 236-242.
18. Barr, B.C., Rowe, J.D., Sverlow, K.W., BonDurant, R.H., Ardans, A.A., Oliver, M.N. and Conrad, P.A., 1994b. Experimental reproduction of bovine fetal Neospora infection and death with a bovine Neospora isolate. J. Vet. Diagn. Invest., 6: 207-215.
19. Barr, B.C., Anderson, M.L., Sverlow, K.W. and Conrad, P.A., 1995. Diagnosis of bovine fetal Neospora infection with an indirect fluorescent antibody test. Vet. Rec., 137: 611-613.
20. Barta, J.R. and Dubey, J.P., 1992. Characterization of anti-Neospora caninum hyperimmune rabbit serum by Western blot analysis and immunoelectron microscopy. Parasitol. Res., 78: 689-694.
21. Baszler, T.V., Knowles, D.P., Dubey, J.P., Gay, J.M., Mathison, B.A. and McElwain, T.F., 1996. Serological diagnosis of bovine neosporosis by Neospora caninum monoclonal antibody-based competitive inhibition ELISA. J. Clin. Microbiol., 34: 1423-1428.
22. Bildfell, R., Davidson, J. and Dubey, J.P., 1994. Neospora-induced protozoal bovine abortion in Prince Edward Island. Can. Vet. J., 35: 122.
23. Bjerkås, I., 1992. Infections with Neospora caninum and Neospora-like parasites in dogs, with special emphasis on infections in Norway. New and emerging infectious diseases. 12th International Symposium Proceedings, World Association of Veterinary Microbiologists, Immunologists and Specialists in Infectious Diseases. Davis, CA, pp. 275-279.
24. Bjerkås, I., Mohn, S.F. and Presthus, J., 1984. Unidentified cyst-forming sporozoon causing encephalomyelitis and myositis in dogs. Z. Parasitenkd., 70: 271-274.
25. Bjerkås, I. and Presthus, J., 1988. Immuno-histochemical and ultrastructural characteristics of a cyst-forming sporozoon associated with encephalomyelitis and myositis in dogs. Acta Pathol. Microbiol. Immunol. Scand., 96: 445-454.
26. Bjerkås, I. and Presthus, J., 1989. The neuropathology in toxoplasmosis-like infection caused by a newly recognized cyst-forming sporozoon in dogs. Acta Pathol. Microbiol. Immunol. Scand., 97: 459-468.
27. Bjerkås, I. and Dubey, J.P., 1991. Evidence that Neospora caninum is identical to the Toxoplasma-like parasite of Norwegian dogs. Acta Vet. Scand., 32: 407-410.
28. Bjerkås, I., Jenkins, M.C. and Dubey, J.P., 1994. Identification and characterization of Neospora caninum tachyzoite antigens useful for diagnosis of neosporosis. Clin. Diagn. Lab. Immunol., 1: 214-221.
29. Björkman, C., Lundén, A., Holmdahl, O.J.M., Barber, J., Trees, A.J. and Uggla, A., 1994a. Neospora caninum in dogs: detection of antibodies by ELISA using an iscom antigen. Parasite Immunol., 16: 643-648.
30. Björkman, C., Lundén, A. and Uggla, A., 1994b. Prevalence of antibodies to Neospora caninum and Toxoplasma gondii in Swedish dogs. Acta Vet. Scand., 35: 445-447.
31. Björkman, C., Holmdahl, J. and Uggla, A., 1996a. An indirect enzyme linked immunoassay (ELISA) for demonstration of antibodies to Neospora caninum in serum and milk cattle. Vet. Parasitol., in press.
32. Björkman, C., Johansson, O., Stenlund, S., Holmdahl, J. and Uggla, A., 1996b. Neospora species infection in a herd of dairy cattle. J. Am. Vet. Med. Assoc., 188: 1441-1444.
33. Blanchard, P.C., Hietala, S.K. and Thurmond, M.C., 1995. Diagnostic interpretation of Neospora serology. Abstract. Proc. 38th Ann. Meet. Am, Assoc. Vet. Lab. Diagn., Sparks, Nevada, p. 40.
34. Boulton, J.G., Gill, P.A., Cook, R.W., Fraser, G.C., Harper, P.A.W. and Dubey, J.P., 1995. Bovine Neospora abortion in north-eastern New South Wales. Aust. Vet. J., 72: 119-120.
35. Braund, K.G., Blagburn, B.L., Toivio-Kinnucan, M., Amling, K.A. and Pidgeon, G.L., 1988. Toxoplasma polymyositis/polyneuropathy - a new clinical variant in two mature dogs. J. Am. Anim. Hosp. Assoc., 24: 93-97.
36. Brindley, P.J., Gazzinelli, R.T., Denkers, E.Y., Davis, S.W., Dubey, J.P., Belfort, R., Martins, M.-C., Silveira, C., Jamra, L., Waters, A.P. and Sher, A., 1993. Differentiation of Toxoplasma gondii from closely related coccidia by riboprint analysis and a surface antigen gene polymerase chain reaction. Am. J. Trop. Med. Hyg., 48: 447-456.
37. Bryan, L.A., Gajadhar, A.A., Dubey, J.P. and Haines, D.M., 1994. Bovine neonatal encephalomyelitis associated with a Neospora sp. protozoan. Can. Vet. J., 35: 111-113.
38. Burkhardt, E., Dubey, J.P., Korte, G. and Bauer, C., 1992. Zwei Erkrankungen infolge einer Infektion mit Neospora caninum bei Hundewelpen in Deutschland. Kleintierpraxis, 37: 701-706.
39. Buxton, D., Caldow, G.L., Maley, S.W. and Marks, J., 1995. Bovine neosporosis in Scotland: interim results of a serological survey. Proceedings, Symposium Neospora abortus bij het rund, 8 November 1995, Morra 2, Drachten, pp. 25-30.
40. Cochrane, S.M. and Dubey, J.P., 1993. Neosporosis in a golden retriever dog from Ontario. Can. Vet. J., 34: 232-233.
41. Cole, R.A., Lindsay, D.S., Dubey, J.P. and Blagburn, B.L., 1993. Detection of Neospora caninum in tissue sections using a murine monoclonal antibody. J. Vet. Diagn. Invest., 5: 579-584.
42. Cole, R.A., Lindsay, D.S., Dubey, J.P., Toivio-Kinnucan, M.A. and Blagburn, B.L., 1994. Characterization of a murine monoclonal antibody generated against Neospora caninum by western blot analysis and immunoelectron microscopy. Am. J. Vet. Res., 55: 1717-1722.
43. Cole, R.A., Lindsay, D.S., Blagburn, B.L. and Dubey, J.P., 1995a. Vertical transmission of Neospora caninum in mice. J. Parasitol., 81: 730-732.
44. Cole, R.A., Lindsay, D.S., Blagburn, B.L., Sorjonen, D.C. and Dubey, J.P., 1995b. Vertical transmission of Neospora caninum in dogs. J. Parasitol., 81: 208-211.
45. Conrad, P.A., Barr, B.C., Sverlow, K.W., Andersen, M., Daft, B., Kinde, H., Dubey, J.P., Munson, L. and Ardans, A., 1993a. In vitro isolation and characterization of a Neospora sp. from aborted bovine foetuses. Parasitology, 106: 239-249.
46. Conrad, P.A., Sverlow, K., Anderson, M., Rowe, J., BonDurant, R., Tuter, G., Breitmeyer, R., Palmer, C., Thurmond, M., Ardans, A., Dubey, J.P., Duhamel, G. and Barr, B., 1993b. Detection of serum antibody responses in cattle with natural or experimental Neospora infections. J. Vet. Diagn. Invest., 5: 572-578.
47. Cuddon, P., Lin, D.-S., Bowman, D.D., Lindsay, D.S., Miller, T.K., Duncan, I.D., deLahunta, A., Cummings, J., Suter, M., Cooper, B., King, J.M. and Dubey, J.P., 1992. Neospora caninum infection in English Springer Spaniel littermates: Diagnostic evaluation and organism isolation. J. Vet. Intern. Med., 6: 325-332.
48. Cummings, J.F., de Lahunta, A., Suter, M.M, and Jacobson, R.H., 1988. Canine protozoan polyradiculoneuritis. Acta Neuropathol., 76: 46-54.
49. Daft, B.M., Barr, B.C., Collins, N., and Sverlow, K., 1996, Neospora encephalomyelitis and polyradiculoneuritis in an aged mare with Cushing's disease. Equine Vet. J., in press.
50. Dannatt, L., Guy, F., and Trees, A.J., 1995. Abortion due to Neospora species in a dairy herd. Vet. Rec., 137: 566-567.
51. Dubey, J.P., 1989. Congenital neosporosis in a calf. Vet. Rec., 125: 486.
52. Dubey, J.P., 1993a. Toxoplasma, Neospora, Sarcocystis, and other tissue cyst-forming coccidia of humans and animals. In: J.P. Kreier (Editor). Parasitic Protozoa. Volume 6. Academic Press., NY, pp. 1-158.
53. Dubey, J.P., 1993b. Protozoal abortion in cattle. J. Am. Vet. Med. Assoc., 203: 1250-1251.
54. Dubey, J.P., Hughes, H.P.A., Lillehoj, H.S., Gamble, H.R. and Munday, B.L., 1987. Placental transfer of specific antibodies during ovine congenital toxoplasmosis. Am. J. Vet. Res., 48: 474-476.
55. Dubey, J.P. and Beattie, C.P., 1988. Toxoplasmosis of animals and man. CRC Press, Boca Raton, FL, pp. 1-200.
56. Dubey, J.P., Carpenter, J.L., Speer, C.A., Topper, M.J. and Uggla, A., 1988a. Newly recognized fatal protozoan disease of dogs. J. Am. Vet. Med. Assoc., 192: 1269-1285.
57. Dubey, J.P., Hattel, A.L., Lindsay, D.S. and Topper, M.J., 1988b. Neonatal Neospora caninum infection in dogs: Isolation of the causative agent and experimental transmission. J. Am. Vet. Med. Assoc., 193: 1259-1263.
58. Dubey, J.P., Leathers, C.W. and Lindsay, D.S., 1989. Neospora caninum-like protozoon associated with fatal myelitis in newborn calves. J. Parasitol., 75: 146-148.
59. Dubey, J.P. and Lindsay, D.S., 1989a. Transplacental Neospora caninum infection in cats. J. Parasitol., 75: 765-771.
60. Dubey, J.P. and Lindsay, D.S., 1989b. Transplacental Neospora caninum infection in dogs. Am. J. Vet. Res., 50: 1578-1579.
61. Dubey, J.P. and Lindsay, D.S., 1989c. Fatal Neospora caninum infection in kittens. J. Parasitol., 75: 148-151.
62. Dubey, J.P. and Lindsay, D.S., 1990a. Neosporosis in dogs. Vet. Parasitol., 36: 147-151.
63. Dubey, J.P. and Lindsay, D.S., 1990b. Neospora caninum induced abortion in sheep. J. Vet. Diagn. Invest., 2: 230-233.
64. Dubey, J.P. and Porterfield, M.L., 1990. Neospora caninum (Apicomplexa) in an aborted equine fetus. J. Parasitol., 76: 732-734.
65. Dubey, J.P., Hartley, W.J. and Lindsay, D.S., 1990a. Congenital Neospora caninum infection in a calf with spinal cord anomaly. J. Am. Vet. Med. Assoc., 197: 1043-1044.
66. Dubey, J.P., Hartley, W.J., Lindsay, D.S. and Topper, M.J., 1990b. Fatal congenital Neospora caninum infection in a lamb. J. Parasitol., 76: 127-130.
67. Dubey, J.P., Higgins, R.J., Smith, J.H. and O'Toole, T.D., 1990c. Neospora caninum encephalomyelitis in a British dog. Vet. Rec., 326: 193-194.
68. Dubey, J.P., Koestner, A. and Piper, R.C., 1990d. Repeated transplacental transmission of Neospora caninum in dogs. J. Am. Vet. Med. Assoc., 197: 857-860.
69. Dubey, J.P., Lindsay, D.S. and Lipscomb, T.P., 1990e. Neosporosis in cats. Vet. Pathol., 27: 335-339.
70. Dubey, J.P., Miller, S., Lindsay, D.S. and Topper, M.J., 1990f. Neospora caninum-associated myocarditis and encephalitis in an aborted calf. J. Vet. Diagn. Invest., 2: 66-69.
71. Dubey, J.P., Acland, H.M. and Hamir, A.N., 1992a. Neospora caninwn (Apicomplexa) in a stillborn goat. J. Parasitol., 78: 532-534.
72. Dubey, J.P., Janovitz, E.B. and Skowronek, A.J., 1992b. Clinical neosporosis in a 4-week-old Hereford calf. Vet. Parasitol., 43: 137-141.
73. Dubey, J.P., Lindsay, D.S., Anderson, M.L., Davis, S.W. and Shen, S.K., 1992c. Induced transplacental transmission of Neospora caninum in cattle. J. Am. Vet. Med. Assoc., 201: 709-713.
74. Dubey, J.P. and Lindsay, D.S., 1993. Neosporosis. Parasitol. Today, 9: 452-458.
75. Dubey, J.P. and de Lahunta, A., 1993. Neosporosis associated congenital limb deformities in a calf. Appl. Parasitol., 34:229-233.
76. Dubey, J.P., Hamir, A.N., Shen, S.K., Thulliez, P. and Rupprecht, C.E., 1993. Experimental Toxoplasma gondii infection in raccoons (Procyon lotor). J. Parasitol., 79: 548-552.
77. Dubey, J.P., Metzger, F.L.J., Hattel, A.L., Lindsay, D.S. and Fritz, D.L., 1995. Canine cutaneous neosporosis: clinical improvement with clindamycin. Vet. Dermatol., 6: 37-43.
78. Dubey, J.P., Lindsay, D.S., Adams, D.S., Gay, J.M., Baszler, T.V., Blagburn, B.L. and Thulliez, P., 1996a. Serologic responses of cattle and other animals infected with Neospora caninum. Am. J. Vet. Res., 57: 329-336.
79. Dubey, J.P., Morales, J.A., Villalobos, P., Lindsay, D.S., Blagburn, B.L. and Topper, M.J., 1996b. Neosporosis-associated abortion in a dairy goat. J. Am. Vet. Med. Assoc., 208: 263-265.
80. Dubey, J.P., Rigoulet, J., Lagourette, P., George, C., Longeart, L. and Le Net, J.L., 1996c. Fatal transplacental neosporosis in a deer (Cervus eldi siamensis) from a zoo. J. Parasitol., 82: 338-339.
81. Ellis, J., Luton, K., Baverstock, P.R., Brindley, P.J., Nimmo, K.A. and Johnson, A.M., 1994. The phytogeny of Neospora caninum. Mol. Biochem. Parasitol., 64: 303-311.
82. Flagstad, A., Jensen, H.E., Bjerkås, I. and Rasmussen, K., 1995. Neospora caninum infection in a litter of Labrador Retriever dogs in Denmark. Acta. Vet. Scand., 36: 387-391.
83. Fritz, D., George, C., Dubey, J.P., Trees, A.J., Barber, J.S., Hopfner, C.L., Mehaut, S., Le Net, J.L. and Longeart, L., 1996. Neospora caninum - associated nodular dermatitis in a middle-aged dog. Canine Pract., in press.
84. Gasser, R.B., Edwards, G. and Cole, R.A., 1993. Neosporosis in a dog. Aust. Vet. Practit., 23: 190-193.
85. Gray, M.L., Harmon, B.G., Sales, L. and Dubey, J.P., 1996. Visceral neosporosis in a 10-year-old horse. J. Vet. Diagn. Invest., 8: 130-133.
86. Greene, C.E., Cook, J.R. and Mahaffey, E.A., 1985. Clindamycin for treatment of Toxoplasma polymyositis in a dog. J. Am. Vet. Med. Assoc., 187: 631-634.
87. Greig, B., Rossow, K.D., Collins, J.E. and Dubey, J.P., 1995. Neospora caninum pneumonia in an adult dog. J. Am. Vet. Med. Assoc., 206: 1000-1001.
88. Guo, Z.-G. and Johnson, A.M., 1995. Genetic comparison of Neospora caninum with Toxoplasma and Sarcocystis by random amplified polymorphic DNA-polymerase chain reaction. Parasitol. Res., 81: 365-370.
89. Harmelin, A., Perl, S., Nyska, A., Yakobson, B., Shpigel, N., Orgad, U. and Dubey, J.P., 1995. Neosporosis-associated bovine abortion in Israel. Vet. Rec., 136: 80.
90. Hartley, W.J. and Bridge, P.S., 1975. A case of suspected congenital Toxoplasma encephalomyelitis in a lamb associated with a spinal cord anomaly. Br. Vet. J., 131: 380-384.
91. Hay, W.H., Shell, L.G., Lindsay, D.S. and Dubey, J.P., 1990. Diagnosis and treatment of Neospora caninum infectiondoga J. Am. Vet Med. Assoc., 197: 87-89.
92. Hemphill, A., Gottstein, B. and Kaufmann, H., 1996. Adhesion and invasion of bovine endothelial cells by Neospora caninum. Parasitology, 112: 183-197.
93. Hemphill, A. and Gottstein, B., 1996. Identification of a major surface protein on Neospora caninum tachyzoites. Parasitol. Res., 82: 497-504.
94. Hilali, M., Lindberg, R., Waller, T. and Wallin, B., 1986. Enigmatic cyst-forming sporozoon in the spinal cord of a dog. Acta Vet. Scand., 27: 623-625.
95. Ho, M.S.Y., Barr, B.C., Marsh, A.E., Anderson, M.L., Rowe, J.D., Tarantal, A.F., Hendrickx, A.G., Sverlow, K., Dubey, J.P. and Conrad, P.A., 1996. Identification of bovine Neospora parasites by PCR amplification and specific small subunit rRNA sequence probe hybridization. J. Clin. Microbiol., 34: 1203-1208.
96. Holmdahl, O.J.M., Mattsson, J.G., Uggla, A. and Johansson, K.E., 1994. The phylogeny of Neospora caninum and Toxoplasma gondii based on ribosomal RNA sequences. FEMS Microbiol. Letters, 119: 187-192.
97. Holmdahl, O.J.M., Björkman, D. and Uggla, A., 1995. A case of Neospora associated bovine abortion in Sweden. Acta Vet. Scand., 36: 279-281.
98. Holmdahl, O.J.M. and Mattsson, J.G., 1996. Rapid and sensitive identification of Neospora caninum by in vitro amplification of the internal transcribed spacer 1. Parasitology, 112: 177-182.
99. Hoskins, J.D., Bunge, M.M., Dubey, J.P. and Duncan, D.E., 1991. Disseminated infection with Neospora caninum in a ten-year-old dog. Cornell Vet., 81: 329-334.
100. 3H uracil. J. Comp. Pathol., 113: 95-100.
101. Jackson, W., de Lahunta, A., Adaska, J., Cooper, B. and Dubey, J.P., 1995. Neospora caninum in an adult dog with progressive cerebellar signs. Progr. Vet. Neurol., 6: 124-127.
102. Jacobson, L.S. and Jardine, J.E., 1993. Neospora caninum infection in three Labrador littermates. J. South Afr. Vet. Assoc., 64: 47-51.
103. Jardine, J.E., 1996. The ultrastructure of bradyzoites and tissue cysts of Neospora caninum in dogs: absence of distinguishing morphological features between parasites of canine and bovine origin. Vet. Parasitol., 62: 231-240.
104. Jardine, J.E. and Dubey, J.P., 1992. Canine neosporosis in South Africa. Vet. Parasitol., 44: 291-294.
105. Jardine, J.E. and Last, R.D., 1993. Neospora caninum in aborted twin calves. J. South Afr. Vet. Assoc., 64: 101-102.
106. Kaufmann, H., Yamage, M., Roditi, I., Dobbelaere, D., Dubey, J.P., Holmdahi, O.J.M., Trees, A. and Gottstein, B., 1996. Discrimination of Neospora caninum from Toxoplasma gondii and other apicomplexan parasites by hybridization and PCR. Mol. Cell. Probes, 10: 289-297.
107. Knowler, C. and Wheeler, S.J., 1995. Neospora caninum infection in three dogs. J. Small Anim. Pract., 36: 172-177.
108. Lally, N.C., Jenkins, M.C. and Dubey, J.P., 1996a. Evaluation of two Neospora caninum recombinant antigens for use in an ELISA for the diagnosis of bovine neosporosis. Clin. Diagn. Lab. Immunol., 3: 275-279.
109. Lally, N.C., Jenkins, M.C. and Dubey, J.P., 1996b. Development of polymerase chain reaction assay for the diagnosis of neosporosis using the Neospora caninum 14-3-3 gene. Mol. Biochem. Parasitol., 75: 169-178.
110. Lathe, C.L., 1994. Neospora caninum in British dogs. Vet. Rec., 134: 532.
111. Lindsay, D.S. and Dubey, J.P., 1989a. In vitro development of Neospora caninum (Protozoa: Apicomplexa) from dogs. J. Parasitol., 75: 163-165.
112. Lindsay, D.S. and Dubey, J.P., 1989b. Evaluation of anti-coccidial drugs' inhibition of Neospora caninum development in cell cultures. J. Parasitol., 75: 990-992.
113. Lindsay, D.S. and Dubey, J.P., 1989c. Immunohistochemical diagnosis of Neospora caninum in tissue sections. Am. J. Vet. Res., 50: 1981-1983.
114. Lindsay, D.S. and Dubey, J.P., 1989d. Neospora caninum (Protozoa: Apicomplexa) infections in mice. J. Parasitol., 75: 772-779.
115. Lindsay, D.S., Blagbum, B.L. and Dubey, J.P., 1990a. Infection of mice with Neospora caninum (Protozoa: Apicomplexa) does not protect against challenge with Toxoplasma gondii. Infect. Immun., 58: 2699-2700.
116. Lindsay, D.S., Dubey, J.P., Upton, S.J. and Ridley, R.K., 1990b. Serological prevalence of Neospora caninum and Toxoplasma gondii in dogs from Kansas. J. Helminthol. Soc. Wash., 57: 86-88.
117. Lindsay, D.S. and Dubey, J.P., 1990a. Effects of sulfadiazine and amprolium on Neospora caninum (Protozoa: Apicomplexa) infections in mice. J. Parasitol., 76: 177-179.
118. Lindsay, D.S. and Dubey, J.P., 1990b. Infections in mice with tachyzoites and bradyzoites of Neospora caninum (Protozoa: Apicomplexa). J. Parasitol., 76: 410-413.
119. Lindsay, D.S. and Dubey, J.P., 1990c. Neospora caninum (Protozoa: Apicomplexa) infections in rats. Can. J. Zool., 68: 1595-1599.
120. Lindsay, D.S., Dubey, J.P. and Blagburn, B.L., 1991. Characterization of a Neospora caninum (Protozoa: Apicomplexa) isolate (NC-3) in mice. J. Alabama Acad. Sci., 62: 1-8.
121. Lindsay, D.S., Blagburn, B.L. and Dubey, J.P., 1992. Factors affecting the survival of Neospora caninum bradyzoites in murine tissues. J. Parasitol., 78: 70-72.
122. Lindsay, D.S., Speer, C.A., Toivio-Kinnucan, M.A., Dubey, J.P, and Blagbum, B.L., 1993. Use of infected cultured cells to compare ultrastructural features of Neospora caninum from dogs and Toxoplasma gondii. Am. J. Vet. Res., 54: 103-106.
123. Lindsay, D.S., Rippey, N.S., Cole, R.A., Parsons, L.C., Dubey, J.P., Tidwell, R.R. and Blagburn, B.L., 1994. Examination of the activities of 43 chemotherapeutic agents against Neospora caninum tachyzoites in cultured cells. Am. J. Vet. Res., 55: 976-981.
124. Lindsay, D.S., Lenz, S.D., Cole, R.A., Dubey, J.P. and Blagbum, B.L., 1995a. Mouse model for central nervous system Neospora caninum infections. J. Parasitol., 81: 313-315.
125. Lindsay, D.S., Rippey, N.S., Powe, T.A., Sartin, E.A., Dubey, J.P. and Blagburn, B.L., 1995b. Abortions, fetal death, and stillbirths in pregnant pygmy goats inoculated with tachyzoites of Neospora caninum. Am. J. Vet. Res., 56: 1176-1180.
126. Lindsay, D.S., Butler, J.M., Rippey, N.S. and Blagburn, B.L., 1996a. Demonstration of synergistic effects of sulfonamides and dihydrofolate reductase/thymidylate synthase inhibitors against Neospora caninum tachyzoites in cultured cells, and characterization of mutants resistant to pyrimethamine. Am. J. Vet. Res., 57: 68-72.
127. Lindsay, D.S., Kelly, E.J., McKown, R., Stein, F.J., Plozer, J., Herman, J., Blagburn, B.L. and Dubey, J.P., 1996b. Prevalence of Neospora caninum and Toxoplasma gondii antibodies in coyotes (Canis latrans) and experimental infections of coyotes with Neospora caninum. J. Parasitol., 82, 657-659.
128. Lindsay, D.S., Steinberg, H., Dubielzig, R.R., Semrad, S.D., Konkle, D.M., Miller, P.E., and Blagbum, B.L., 1996c. Central nervous system neosporosis in a foal. J. Vet. Diagn. Invest., in press.
129. Lindsay, D.S., Butler, J.M., Blagbum, B.L., 1996d. Efficacy of decoquinate against Neospora caninum tachyzoites in cell cutures. Vet. Parasitol., in press.
130. Long, M.T. and Baszler, T.V., 1996. Fetal loss in Balb/c mice infected with Neospora caninum. J. Parasitol., in press.
131. Marsh, A.E., Barr, B.C., Svertow, K., Ho, M., Dubey, J.P. and Conrad, P.A., 1995. Sequence analysis and comparison of ribosomal DNA from bovine Neospora to similar coccidial parasites. J. Parasitol., 81: 530-535.
132. Marsh, A.E., Barr, B.C., Madigan, J.E. and Conrad, P.A.. 1996. In vitro cultivation and characterization of a Neospora isolate obtained from a horse with protozoal myeloencephalitis. Proc. Am. Soc. Parasitol. and The Society of Protozoologists, Tucson, Arizona, 11-15 June, 1996. Abstract No. 114.
133. Mayhew, I.G., Smith, K.C., Dubey, J.P., Gatward, L.K. and McGlennon, N.J., 1991. Treatment of encephalomyelitis due to Neospora caninum in a litter of puppies. J. Small Anim. Pract., 32: 609-612.
134. McAllister, M., 1993. Protozoal abortion in cattle. J. Am. Vet. Med. Assoc., 203: 1251.
135. McAllister, M.M., Huffman, E.M., Hietala, S.K., Conrad, P.A., Anderson, M.L. and Salman, M.O., 1996a. Evidence suggesting a point source exposure in an outbreak of bovine abortion due to neosporosis. J. Vet. Diagn. Invest, 8: 355-357.
136. McAllister, M.M., McGuire, A.M., Jolley, W.R., Lindsay, D.S., Trees, A.J. and Stobart, R.H., 1996b. Experimental neosporosis in pregnant ewes and their offspring. Vet. Pathol., in press.
137. McAllister, M.M., Parmley, S.F., Weiss, L.M., Welch, V.J. and McGuire, A.M., 1996c. An immunohistochemical method for detecting bradyzoite antigen (BAG5) in Toxoplasma gondii-infected tissues cross-reacts with a Neospora caninumbradyzoite antigen. J. Parasitol., 82: 354-355.
138. McGlennon, N.J., Jefferie., A.R. and Casas, C., 1990. Polyradiculo-neuritis and polymyositis due to a Toxoplasma-like protozoan: Diagnosis and treatment. J. Small Anim. Pract., 31: 102-104.
139. McGuire, A.M., McAllister, M.M. and Jolley, W.R., 1996. Separation and cryopreservation of Neospora caninum tissue cysts from murine brains. J. Parasitol., in press.
140. McIntosh, D.W. and Haines, D.M., 1994. Neospora infection in an aborted fetus in British Columbia. Can. Vet. J., 35: 114-115.
141. McNamee, P.T. and Jeffrey, M., 1994. Neospora-associated bovine abortion in Northern Ireland. Vet. Rec., 134: 48.
142. McNamee, P.T., Trees, A.J., Guy, F., Moffett, D. and Kilpatrick, D., 1996. The diagnosis and prevalence of neosporosis in Northern Ireland cattle. Vet. Rec., 138: 419-420.
143. Moen, A.R. and Wouda, W., 1995. Field experiences with bovine Neospora abortion in Dutch dairy herds. Proceedings, Symposium Neospora Abortus Bij Het Rund, 8 November 1995, Morra 2, Drachten, pp. 11-17.
144. Moen, A.R., Wouda, W. and van Werven, T., 1995a. Clinical and sero-epidemiological follow-up study in four dairy herds with an outbreak of Neospora abortion. Proc. Dutch Society for Veterinary Epidemiology and Economics. Lelystad, 13 December 1995, pp. 93-103.
145. Morales, J.A., Dubey, J.P., Rodriguez, F., Esquivel, R.L. and Fritz, D., 1995. Neosporosis and toxoplasmosis-associated paralysis in dogs in Costa Rica. Appl. Parasitol., 36: 179-184.
146. Munday, B.L., Dubey, J.P. and Mason, R.W., 1990. Neospora caninum infection in dogs. Aust. Vet. J., 67: 76.
147. Nietfeld, J.C., Dubey, J.P., Anderson, M.L., Libal, M.C., Yaeger, M.J. and Neiger, R.D., 1992. Neospora-like protozoan infection as a cause of abortion in dairy cattle. J. Vet. Diagn, Invest., 4: 223-226.
148. O'Toole, D. and Jeffrey, M., 1987. Congenital sporozoan encephalomyelitis in a calf. Vet. Rec., 121: 563-566.
149. Obendorf, D.L., Murray, N., Veldhuis, G., Munday, B.L. and Dubey, J.P., 1995. Abortion caused by neosporosis in cattle. Aust. Vet. J., 72: 117-118.
150. Odin, M. and Dubey, J.P., 1993. Sudden death associated with Neospora caninum-myocarditis in a dog. J. Am. Vet. Med. Assoc., 203: 831-833.
151. Ogino, H., Watanabe, E., Watanabe, S., Agawa, H., Narita, M., Haritani, M. and Kawashima, K., 1992. Neosporosis in the aborted fetus and newborn calf. J. Comp. Pathol., 107: 231-237.
152. Otter, A., Jeffrey, M., Griffiths, I.B. and Dubey, J.P., 1995. A survey of the incidence of Neospora caninum infection in aborted and stillborn bovine fetuses in England and Wales. Vet. Rec., 136: 602-606.
153. Paré, J., Thurmond, M.C. and Hietala, S.K., 1994. Congenital Neospora infection in dairy cattle. Vet. Rec., 134: 531-532.
154. Paré, J., Hietala, S.K. and Thurmond, M.C., 1995a. Interpretation of an indirect fluorescent antibody test for diagnosis of Neospora sp. infection in cattle. J. Vet. Diagn. Invest., 7: 273-275.
155. Paré, J., Hietala, S.K. and Thurmond, M.C., 1995b. An enzyme-linked immunosorbent assay (ELISA) for serological diagnosis of Neospora sp. infection in cattle. J. Vet. Diagn. Invest., 7: 352-359.
156. Paré, J., Thurmond, M.C. and Hietala, S.K., 1996a. Congenital Neospora caninum infection in dairy cattle and associated calfhood mortality. Can. J. Vet. Res., in press.
157. Paré, J., Thurmond, M.C., and Hietala, S.K., 1996b. Neospora caninum antibodies in cows during pregnancy as a predictor of congenital infection and abortion. J. Parasitol., in press.
158. Parish, S.M., Maag-Miller, L., Besser, T.E., Weidner, J.P., McElwain, T., Knowles, D.P. and Leathers, C.W., 1987. Myelitis associated with protozoal infection in newborn calves. J. Am. Vet. Med. Assoc., 191: 1599-1600.
159. Payne, S. and Ellis, J., 1996. Detection of Neospora caninum DNA by the polymerase chain reaction. Int. J. Parasitol., 26: 347-351.
160. Poncelet, L., Bjerkas, I., Charlier, G., Coignoul, F., Losson, B. and Balligand, M., 1990a. Confirmation de la présence de Neospora caninum en Belgique. Ann. Méd. Vét., 134: 501-503.
161. Poncelet, L., Coignoul, F., Fontaine, J. and Balligand, M., 1990b. Infestation de chiots par Neospora canis en Belgique et en France? Ann, Méd. Vét., 134: 167-171.
162. Pumarola, M., Añor, S., Ramis, A.J., Borràs, D., Gorraiz, J. and Dubey, J.P., 1996. Neospora caninum infection in a Napolitan mastiff dog from Spain. Vet. Parasitol., 64: 315-317.
163. Reichel, M.P. and Drake, J.M., 1996. The diagnosis of Neospora caninum abortions in cattle. N.Z. Vet. J., in press.
164. Rogers, D.G., Grotelueschen, D.M., Anderson, M.L., McCullough, M.S., Shain, W.S. and Dubey, J.P., 1993. Endemic protozoal abortions in a dairy cow herd. Agri-Practice, 14: 16-21.
165. Rudbäck, E., Mannonen, J., Nikander, S. and Henriksson, K., 1991. Neopsora caninum-uusi parasiitti Suomessa? Suomen Eläinlääkärilehti, 97: 526-529.
166. Ruehlmann, D., Podell, M., Oglesbee, M. and Dubey, J.P., 1995. Canine neosporosis: A case report and literature review. J. Am. Anim. Hosp. Assoc., 31: 174-183.
167. Sheahan, B.J., Caffrey, J.F., Dubey, J.P. and McHenry, D.F., 1993. Neospora caninum encephalomyelitis in seven dogs. Irish Vet. J., 46: 3-7.
168. Shivaprasad, H.L., Ely, R. and Dubey, J.P., 1989. A Neospora-like protozoon found in an aborted bovine placenta. Vet. Parasitol., 34: 145-148.
169. Speer, C.A. and Dubey, J.P., 1989. Ultrastructure of tachyzoites, bradyzoites, and tissue cysts of Neospora caninum. J. Protozool., 36: 458-463.
170. Sréter, T., Sebestyén, P. and Dubey, J.P., 1992. Neosporosis in a dog in Hungary. Parasit. Hung., 25: 5-8.
171. Stenlund, S., Björkman, C., Holmdahl, O.J.M., Kindahl, H. and Uggla, A., 1996. Characterization of a Swedish bovine isolate of Neospora caninum. Parasitol. Res., in press.
172. Thilsted, J.P. and Dubey, J.P., 1989. Neosporosis-like abortions in a herd of dairy cattle. J. Vet. Diagn. Invest., 1: 205-209.
173. Thomton, R.N., Thompson, E.J. and Dubey, J.P., 1991. Neospora abortion in New Zealand cattle. N.Z. Vet. J., 39: 129-133.
174. Thornton, R.N., Gajadhar, A. and Evans, J., 1994. Neospora abortion epidemic in a dairy herd. N.Z. Vet. J., 42: 190-191.
175. Thurmond, M. and Hietala, S., 1995. Strategies to control Neospora infection in cattle. Bovine Pract., No. 29: 60-63.
176. Thurmond, M., Hietala, S., Blanchard, P. and DeBey, S., 1995a. Congenital transmission of Neospora caninum in herds experiencing endemic or epidemic abortion. Abstract. Proc. 38th Am. Meet. Am. Assoc. Vet. Lab. Diagn., Sparks, Nevada, p. 97.
177. Thurmond, M.C., Anderson, M.L. and Blanchard, P.C., 1995b. Secular and seasonal trends of Neospora abortion in California dairy cows. J. Parasitol., 81: 364-367.
178. Trees, A.J., Guy, F., Tennant, B.J., Balfour, A.H. and Dubey, J.P., 1993. Prevalence of antibodies to Neospora caninum in a population of urban dogs in England. Vet. Rec., 132: 125-126.
179. Trees, A.J., Guy, F., Low, J.C., Roberts, L., Buxton, D. and Dubey, J.P., 1994. Serological evidence implicating Neospora species as a cause of abortion in British cattle. Vet. Rec., 134: 405-407.
180. Uggla, A., Dubey, J.P., Funkquist, B. and Segall, T., 1989a. Fatal Neospora caninum-infektion hos riesenschnauzer. Svensk Veterinärtidning, 41: 271-274.
181. Uggta, A., Dubey, J.P., Lundmark, G. and Olson, P., 1989b. Encephalomyelitis and myositis in a Boxer puppy due to a Neospora-like infection. Vet. Parasitol., 32: 255-260.
182. Umemura, T., Shiraki, K., Monta, T., Shimada, A., Haratani, M., Kobayashi, M. and Yamagata, S., 1992. Neosporosis in a dog: The first case report in Japan. J. Vet. Med. Sci., 54: 157-159.
183. Williams, D.J.L., McGarry, J., Guy, F., Barber, J.S. and Trees, A.J., 1996. A novel ELISA for detection of Neospora-specific antibodies in cattle. Vet. Rec., in press.
184. Wolf, M., Cachin, M., Vandevelde, M., Tipold, A. and Dubey, J.P., 1991. Zur klinischen Diagnostik des protozoären Myositissyndroms (Neospora caninum) des Welpen. Tierarztl. Prax., 19: 302-306.
185. Woods, L.W., Anderson, M.L., Swift, P.K. and Sverlow, K.W., 1994. Systemic neosporosis in a California black-tailed deer (Odocoileus hemionus columbianus). J. Vet. Diagn. Invest., 6: 508-510.
186. Wouda, W., van den Ingh, T.S.G.A.M., van Knapen, F., Sluyter, F.J.H., Koeman, J.P. and Dubey, J.P., 1992. Neospora abortus bij net rund in Nederland. Tijdschr. Diergeneeskd., 117: 599-602.
187. Wouda, W., de Jong, J.K., Van Knapen, F. and Walvoort, H.C., 1993. Neospora caninum als oorzaak van verlammingsverschijnselen bij jonge honden. Tijdschr. Diergeneeskd., 118: 397-401.
188. Wouda, W., de Gee, A.L.W., Moen, A.R. and Van Knapen, F., 1995. Laboratory experiences with bovine Neospora abortion in Dutch dairy herds. Proceedings, Symposium Neospora Abortus Bij Het Rund, 8 November 1995, Morra 2, Drachten, pp. 3-9.
189. Wouda, W., Moen, A.R., Visser, I.J.R. and van Knapen, F., 1996. Bovine fetal neosporosis: A comparison of epizootic and sporadic abortion cases and different age classes with regards to lesion severity and immunohistochemical identification in brain, heart and liver. J. Vet. Diagn. Invest., in press.
190. Yaeger, M.J., Shawd-Wessels, S. and Leslie-Steen, P., 1994. Neospora abortion storm in a midwestem dairy. J. Vet. Diagn. Invest., 6: 506-508.
191. Yamage, M., Flechtner, O. and Gottstein, B., 1996. Neospora caninum: specific oligonucleotide primers for the detection of brain 'cyst' DNA of experimentally infected nude mice by the polymerase chain reaction (PCR). J. Parasitol., 82: 272-279.
192. Yamane, I., Thomford, J.W., Gardner, I.A., Dubey, J.P., Levy, M. and Conrad, P.A., 1993. Evaluation of the indirect fluorescent antibody test for diagnosis of Babesia gibxoni infections in dogs. Am. J. Vet. Res., 54: 1579-1584.
193. Yamane, I., Kokuho, T., Shimura, K., Eto, M., Haritani, M., Ouchi, Y., Sverlow, K.W. and Conrad, P.A., 1996. In vitro isolation of a bovine Neospora in Japan. Vet. Rec., 138: 652.