Therapeutic silencing of microRNA-122 in primates with chronic hepatitis C virus infection

Science

Volumn 327, Issue 5962, 2010, Pages 198-201

  Lanford, Robert E ^a   Hildebrandt Eriksen, Elisabeth S ^b   Petri, Andreas ^b   Persson, Robert ^b   Lindow, Morten ^b   Munk, Martin E ^b   Kauppinen, Sakari ^b,c   Rum, Henrik ^b  

^a SOUTHWEST NATIONAL PRIMATE RESEARCH CENTER   (United States)

^b Santaris Pharma ^*  (Denmark)

^c AALBORG UNIVERSITY   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIVIRUS AGENT; INTERFERON; LOCKED NUCLEIC ACID MODIFIED OLIGONUCLEOTIDE; MICRORNA; SPC 3649; UNCLASSIFIED DRUG;

GENE EXPRESSION; GENETIC ANALYSIS; NUCLEIC ACID; PATHOLOGY; RNA;

ANTIVIRAL THERAPY; AREA UNDER THE CURVE; ARTICLE; CHIMPANZEE; DOWN REGULATION; DRUG BLOOD LEVEL; DRUG HALF LIFE; DRUG MEGADOSE; GENE SILENCING; HEPATITIS C; HEPATITIS C VIRUS; LIVER BIOPSY; LOW DRUG DOSE; MAXIMUM PLASMA CONCENTRATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; TRANSCRIPTOMICS; TREATMENT RESPONSE; VIREMIA; VIRUS LOAD; VIRUS RESISTANCE;

ANIMALS; ANTIVIRAL AGENTS; CHEMOKINE CXCL10; CHOLESTEROL; DISEASE MODELS, ANIMAL; DRUG EVALUATION, PRECLINICAL; DRUG RESISTANCE, VIRAL; FEMALE; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; HEPACIVIRUS; HEPATITIS C, CHRONIC; INTERFERONS; LIVER; MALE; MICRORNAS; PAN TROGLODYTES; PHOSPHOROTHIOATE OLIGONUCLEOTIDES; RNA, MESSENGER; RNA, VIRAL; VIRAL LOAD; VIREMIA;

ANIMALIA; HEPATITIS C VIRUS; PAN; PRIMATES;

EID: 74249112787     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1178178     Document Type: Article

References (20)

1. F. V. Chisari, Nature 436, 930 (2005).
2. J. J. Feld, J. H. Hoofnagle, Nature 436, 967 (2005).
3. C. L Jopling, M. K. Yi, A. M. Lancaster, S. M. Lemon, P. Sarnow, Science 309,1577 (2005).
4. C. L. Jopling, S. Schütz, P. Sarnow, Cell Host Microbe 4, 77 (2008).
5. G. Randall et al, Proc. Natl. Acad. Sci. U.S.A. 104, 12884 (2007).
6. J. Elmén et al, Nature 452, 896 (2008).
7. M. Sarasin-Filipowicz, J. Krol, I. Markiewicz, M. H. Heim, W. Filipowicz, Nat. Med. 15, 31 (2009).
8. C. M. Chen et al, Antimicrob. Agents Chemother. 51, 4290 (2007).
9. R. E. Lanford et al, Hepatology 46, 999 (2007).
10. R. E. Lanford et al, Hepatology 43, 961 (2006).
11. L. M. Chen et al, Gastroenterology 128, 1437 (2005).
12. M. Lagging et al; DITTO-HCV Study Group, Hepatology 44, 1617 (2006).
13. M. Diago et al, Gut 55, 374 (2006).
14. D. Butera et al, Blood 106, 1175 (2005).
15. A. I. Romero et al., J. Infect. Dis. 194, 895 (2006).
16. M. Sarasin-Filipowicz et al, Proc. Natl. Acad. Sci. U.S.A. 105, 7034 (2008).
17. J. J. Feld et al, Hepatology 46, 1548 (2007).
18. C. B. Bigger et al, J. Virol. 78, 13779 (2004).
19. J. Elmén et al, Nucleic Acids Res. 36, 1153 (2008).
20. We thank D. Chavez, B. Guerra, and H. Lee for excellent technical assistance; K. Brasky for veterinary support; L. Giavedoni for immunological analyses; P. Giclas for complement analyses; and E. Dick for pathology examinations. Supported by a grant from the Danish National Advanced Technology Foundation (S.K.); the primate studies performed at the Southwest National Primate Research Center are supported by NIH base grant P51 RR13986 and by the National Center for Research Resources (Research Facilities Improvement Program grant C06 RR12087). The expression microarray data have been deposited in the ArrayExpress repository under accession number E-MEXP-2375. The GEO accession number for the sequencing data is GSE18919. E.S.H-E., A.P., and S.K. have a pending patent on the method of treatment.