Utilization of lysozyme charge ladders to examine the effects of protein surface charge distribution on binding affinity in ion exchange systems

Langmuir

Volumn 26, Issue 2, 2010, Pages 759-768

  Chung, Wai Keen ^a,b   Evans, Steven T ^a,b   Freed, Alexander S ^a,b   Keba, James J ^c   Baer, Zachary C ^a,b   Rege, Kaushal ^d   Cramer, Steven M ^a,b  

^a RENSSELAER POLYTECHNIC INSTITUTE   (United States)

^b RENSSELAER POLYTECHNIC INSTITUTE   (United States)

^c GENENTECH INC   (United States)

^d Arizona State University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETIC ANHYDRIDES; BINDING AFFINITIES; CAPILLARY ZONE ELECTROPHORESIS; CATION EXCHANGE CHROMATOGRAPHY; CATION EXCHANGES; COELUTION; ELECTROSPRAY IONIZATION MASS SPECTROMETRY; ELECTROSTATIC POTENTIAL MAPS; ELUTION ORDER; ION EXCHANGE SEPARATION; ION EXCHANGE SYSTEMS; LYSINE RESIDUES; MODIFIED PROTEINS; NET CHARGES; POSITIVE CHARGES; POSITIVE SURFACE CHARGE; PROTEIN BINDING; PROTEIN CHARGE; PROTEIN RETENTION; PROTEIN SURFACE; RETENTION TIME;

ACETYLATION; AMINO ACIDS; BINDING ENERGY; BIOCHEMISTRY; CAPILLARY ELECTROPHORESIS; CHARGED PARTICLES; CHROMATOGRAPHIC ANALYSIS; ELECTROSPRAY IONIZATION; ION EXCHANGERS; MASS SPECTROMETERS; MASS SPECTROMETRY; POSITIVE IONS; PROTEINS;

ION EXCHANGE;

ACETIC ANHYDRIDE; LYSINE; LYSOZYME;

ACETYLATION; ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; ION EXCHANGE; ION EXCHANGE CHROMATOGRAPHY; MASS SPECTROMETRY; PROTEIN BINDING; THEORETICAL MODEL;

ACETIC ANHYDRIDES; ACETYLATION; CHROMATOGRAPHY, ION EXCHANGE; ION EXCHANGE; LYSINE; MASS SPECTROMETRY; MODELS, MOLECULAR; MODELS, THEORETICAL; MURAMIDASE; PROTEIN BINDING;

EID: 74249092297     PISSN: 07437463     EISSN: None     Source Type: Journal    
DOI: 10.1021/la902135t     Document Type: Article

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