Purification and characterization of a multienzyme complex produced by Paenibacillus curdlanolyticus B-6

Applied Microbiology and Biotechnology

Volumn 85, Issue 3, 2010, Pages 573-580

  Pason, Patthra ^a,b   Kosugi, Akihiko ^a   Waeonukul, Rattiya ^a,b   Tachaapaikoon, Chakrit ^b   Ratanakhanokchai, Khanok ^b   Arai, Takamitsu ^a   Murata, Yoshinori ^a   Nakajima, Jun ^a   Mori, Yutaka ^a  

^a JAPAN INTERNATIONAL RESEARCH CENTER FOR AGRICULTURAL SCIENCES   (Japan)

^b KING MONGKUT'S UNIVERSITY OF TECHNOLOGY THONBURI   (Thailand)

Author keywords

Cellulase; Hemicellulase; Multienzyme complex; Paenibacillus curdlanolyticus; Xylanase

Indexed keywords

AMINO ACID SEQUENCE; CARBOHYDRATE-BINDING MODULES; CARBOXYMETHYL CELLULASE; CELLULOSOME ASSEMBLY; CELLULOSOMES; COHESINS; DEGRADATION ACTIVITY; EXTRACELLULAR; FUNCTIONAL DOMAINS; GLYCOSYL HYDROLASES; HEMICELLULASES; MULTIENZYME COMPLEXES; POLYACRYLAMIDE GELS; SCAFFOLDING PROTEINS; SUPERNATANTS; TERMINAL SIDES; XYLANASE ACTIVITY; XYLANASES;

AMINO ACIDS; CARBOHYDRATES; CHROMATOGRAPHIC ANALYSIS; CLONING; ENZYMES; ORGANIC ACIDS; PURIFICATION;

COMPLEXATION;

260 KDA PROTEIN; 280 KDA PROTEIN; 48 KDA PROTEIN; 60 KDA PROTEIN; 65 KDA PROTEIN; ASPARAGINE; CARBOHYDRATE BINDING PROTEIN; CELLULASE; CELLULOSOME; GLYCINE; GLYCOPROTEIN; GLYCOSIDASE; MULTIENZYME COMPLEX; POLYACRYLAMIDE GEL; SCAFFOLD PROTEIN; UNCLASSIFIED DRUG; XYLAN ENDO 1,3 BETA XYLOSIDASE;

AMINO ACID; BACTERIUM; BIOCHEMISTRY; CELLULOSE; CHROMATOGRAPHY; DEGRADATION; ENZYME ACTIVITY; EXTRACTION METHOD; MIGRATORY BEHAVIOR; PROTEIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; GEL ELECTROPHORESIS; GEL FILTRATION CHROMATOGRAPHY; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PAENIBACILLUS; PAENIBACILLUS CURDLANOLYTICUS B 6; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN STRUCTURE; ZYMOGRAPHY;

CELLULASES; CHROMATOGRAPHY; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDO-1,4-BETA XYLANASES; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PAENIBACILLUS; PROTEIN STRUCTURE, TERTIARY; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID;

PAENIBACILLUS CURDLANOLYTICUS;

EID: 74149093710     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-2117-2     Document Type: Article

References (24)

1. Aspinall GO (1980) Chemistry of cell-wall polysaccharides. In: Preiss J (ed) The biochemistry of plants. Academic, New York, pp 473-500
2. EA Bayer J-P Belaich Y Shoham R Lamed 2004 The cellulosomes: multienzyme machines for degradation of plant cell wall polysaccharides Annu Rev Microbiol 58 521 554 10.1146/annurev.micro.57.030502.091022 1:CAS:528:DC%2BD2cXhtVejsr3M (Pubitemid 39551996)
3. B Berg BV Hofsten G Pettersson 1972 Growth and cellulase formation by Cellvibrio fulvus J Appl Bacteriol 35 201 214 1:CAS:528:DyaE38Xlt1ektrk%3D
4. AL Demain M Newcomb JHD Wu 2005 Cellulase, clostridium, and ethanol Microbiol Mol Biol Rev 69 124 154 10.1128/MMBR.69.1.124-154.2005 1:CAS:528:DC%2BD2MXjtVOhtLg%3D (Pubitemid 40360711)
5. S-Y Ding EA Bayer D Steiner Y Shoham RA Lamed 1999 Novel cellulosomal scaffoldin from Acetivibrio cellulolyticus that contains a family-9 glycosyl hydrolase J Bacteriol 181 6720 6729 1:CAS:528:DyaK1MXntFeqtL8%3D
6. RH Doi A Kosugi 2004 Cellulosomes: plant-cell-wall-degrading enzyme complexes Nat Rev Microbiol 2 541 551 10.1038/nrmicro925 1:CAS:528: DC%2BD2cXkvVSru7Y%3D (Pubitemid 39206267)
7. RH Doi A Kosugi K Murashima Y Tamaru SO Han 2003 Cellulosomes from mesophilic bacteria J Bacteriol 185 5907 5914 10.1128/JB.185.20.5907-5914.2003 1:CAS:528:DC%2BD3sXotFOitbk%3D (Pubitemid 37248441)
8. S Ebina K Matsubara K Nagayama M Yamaki T Gotoh 1995 Carbohydarte gluing, an architectural mechanism in the supramolecular structure of an annelid giant hemoglobin Proc Natl Acad Sci U S A 92 7367 7371 10.1073/pnas.92.16.7367 1:CAS:528:DyaK2MXntlKmtLc%3D
9. L Gal S Pages C Gaudin A Belaich C Reverbel-Leroy C Tardif JP Belaich 1997 Characterization of the cellulolytic complex (cellulosome) produced by Clostridium cellulolyticum Appl Environ Microbiol 63 903 909 1:CAS:528:DyaK2sXhsV2ju7Y%3D
10. MJ Gosalbes JA Perez-Gonzalez R Gonzalez A Navarro 1991 Two beta-glycanase genes are clustered in Bacillus polymyxa: molecular cloning, expression, and sequence analysis of genes encoding a xylanase and an endo-beta-(1, 3)-(1, 4)-glucanase J Bacteriol 173 7705 7710 1:CAS:528: DyaK3sXjtlOgsw%3D%3D
11. ZQ Jiang W Deng XT Li ZL Ai LT Li I Kusakabe 2005 Characterization of a novel, ultra-large xylanolytic complex (Xylanosome) from Streptomyces olivaceoviridis E-86 Enzyme Microb Technol 36 923 929 10.1016/j.enzmictec.2005. 01.023 1:CAS:528:DC%2BD2MXjs1Kku7s%3D (Pubitemid 40591711)
12. S Jindou I Borovok MT Rincon HJ Flint DA Antonopoulos ME Berg BA White EA Bayer R Lamed 2006 Conservation and divergence in cellulosome architecture between two strains of Ruminococcus flavefaciens J Bacteriol 188 7971 7976 10.1128/JB.00973-06 1:CAS:528:DC%2BD28Xht1ahsb3N (Pubitemid 44764534)
13. UK Laemmli 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 277 680 685 10.1038/227680a0
14. R Lamed EA Bayer 1988 The cellulosome of Clostridium thermocellum Adv Appl Microbiol 33 1 46 10.1016/S0065-2164(08)70203-X
15. R Lamed E Setter EA Bayer 1983 Characterization of a cellulose-binding, cellulase-containing complex in Clostridium thermocellum J Bacteriol 156 828 836 1:CAS:528:DyaL2cXjt1Cm
16. LL Lin JA Thomson 1991 An analysis of the extracellular xylanases and cellulases of Butyrivibrio fibrisolvens H17c FEMS Microbiol Lett 68 197 204 10.1111/j.1574-6968.1991.tb04596.x 1:STN:280:DyaK387jslKrsg%3D%3D
17. LR Lynd PJ Weimer WH van Zyl IS Pretorius 2002 Microbial cellulose utilization: fundamentals and biotechnology Microbiol Mol Biol Rev 66 506 577 10.1128/MMBR.66.3.506-577.2002 1:CAS:528:DC%2BD38XnsFOitrk%3D (Pubitemid 35005827)
18. E Morag EA Bayer R Lamed 1990 Relationship of cellulosomal and noncellulosomal xylanases of Clostridium thermocellum to cellulose-degradating enzymes J Bacteriol 172 6098 6105 1:CAS:528:DyaK3MXhvFyluw%3D%3D
19. P Pason KL Kyu K Ratanakhonokchai 2006 Paenibacillus curdlanolyticus stain B-6 xylanolytic-cellulolytic enzyme system that degrades insoluble polysaccharides Appl Environ Microbiol 72 2483 2490 10.1128/AEM.72.4.2483-2490. 2006 1:CAS:528:DC%2BD28XktFagtbw%3D
20. M Pohlschroder SB Leschine E Canale-parola 1994 Multicomplex cellulase-xylanase system of Clostridium papyrosolvens C7 J Bacteriol 176 70 76 1:STN:280:DyaK2c7htVansQ%3D%3D
21. K Ratanakhanokchai KL Kyu M Tanticharoen 1999 Purification and properties of a xylan-binding endoxylanase from alkaliphilic Bacillus sp. strain K-1 Appl Environ Microbiol 65 694 697 1:CAS:528:DyaK1MXpvFCqsA%3D%3D
22. JA Thomson 1993 Molecular biology of xylan degradation FEMS Microbiol Rev 104 65 82 10.1111/j.1574-6968.1993.tb05864.x 1:CAS:528:DyaK3sXps1OjtA%3D%3D
23. R Waeonukul P Pason KL Kyu K Sakka A Kosugi Y Mori K Ratanakhanokchai 2009 Cloning, sequencing, and expression of the gene encoding a multidomain endo-β-1, 4-xylanase from Paenibacillus curdlanolyticus B-6, and characterization of the recombinant enzyme J Microbiol Biotechnol 19 277 285 1:CAS:528:DC%2BD1MXlt1GisL0%3D
24. Q Xu W Gao S-Y Ding R Kenig Y Shoham EA Bayer R Lamed 2003 The cellulosome system of Acetivibrio cellulolyticus includes a novel type of adaptor protein and a cell surface anchoring protein J Bacteriol 185 4548 4557 10.1128/JB.185.15.4548-4557.2003 1:CAS:528:DC%2BD3sXlvVCiu74%3D (Pubitemid 36890502)