Characterization of kinase target phosphorylation consensus motifs using peptide SPOT arrays

Methods in Molecular Biology

Volumn 570, Issue , 2009, Pages 187-195

  Leung, Genie C ^a   Murphy, James M ^b   Briant, Doug ^c   Sicheri, Frank ^c  

^a PRINCESS MARGARET HOSPITAL   (Canada)

^b Institute of Medical Research   (Australia)

^c MOUNT SINAI HOSPITAL   (Canada)

Author keywords

Kinase specificity; peptide SPOT arrays; phosphorylation consensus motifs

Indexed keywords

PEPTIDE; PROTEIN KINASE;

ANIMAL; BIOLOGICAL MODEL; ENZYME ASSAY; ENZYME SPECIFICITY; HUMAN; METABOLISM; METHODOLOGY; PEPTIDE MAPPING; PHOSPHORYLATION; PROTEIN MICROARRAY; PROTEIN MOTIF; REVIEW; SYNTHESIS;

AMINO ACID MOTIFS; ANIMALS; ENZYME ASSAYS; HUMANS; MODELS, BIOLOGICAL; PEPTIDE MAPPING; PEPTIDES; PHOSPHORYLATION; PROTEIN ARRAY ANALYSIS; PROTEIN KINASES; SUBSTRATE SPECIFICITY;

EID: 73949148230     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60327-394-7_7     Document Type: Article

References (7)

1. Manning, G., Whyte, D. B., Martinez, R., Hunter, T., and Sudarsanam, S. (2002) The protein kinase complement of the human genome. Science, 298, 1912-34.
2. Kennelly, P. J., and Krebs, E. G. (1991) Consensus sequences as substrate specificity determinants for protein kinases and protein phosphatases. J Biol Chem, 266, 15555-8.
3. Songyang, Z., and Cantley, L. C. (1995) Recognition and specificity in protein tyrosine kinase-mediated signalling. Trends Biochem Sci, 20, 470-5.
4. Songyang, Z., Lu, K. P., Kwon, Y. T., Tsai, L. H., Filhol, O., Cochet, C., Brickey, D. A., Soderling, T. R., Bartleson, C., Graves,D. J., DeMaggio, A. J., Hoekstra, M. F., Blenis, J., Hunter, T., and Cantley, L. C. (1996) A structural basis for substrate specificities of protein Ser/Thr kinases: Primary sequence preference of casein kinases I and II, NIMA, phosphorylase kinase, calmodulindependent kinase II, CDK5, and Erk1. Mol Cell Biol, 16, 6486-93.
5. Frank, R. (2002) The SPOT-synthesis technique. Synthetic peptide arrays on membrane supports-principles and applications. J Immunol Methods, 267, 13-26.
6. Lizcano, J. M., Deak, M., Morrice, N., Kieloch, A., Hastie, C. J., Dong, L., Schutkowski, M., Reimer, U., and Alessi, D. R. (2002) Molecular basis for the substrate specificity of NIMA-related kinase-6 (NEK6). Evidence that NEK6 does not phosphorylate the hydrophobic motif of ribosomal S6 protein kinase and serum-and glucocorticoidinduced protein kinase in vivo. J Biol Chem, 277, 27839-49.
7. Leung, G. C., Ho, C. S., Blasutig, I. M., Murphy, J. M., and Sicheri, F. (2007) Determination of the Plk4/Sak consensus phosphorylation motif using peptide spots arrays. FEBS Lett, 581, 77-83.