N-acetylcysteine prevents nitric oxide-induced chondrocyte apoptosis and cartilage degeneration in an experimental model of osteoarthritis

Journal of Orthopaedic Research

Volumn 28, Issue 2, 2010, Pages 156-163

  Nakagawa, Shuji ^a   Arai, Yuji ^a   Mazda, Osam ^a   Kishida, Tsunao ^a   Takahashi, Kenji A ^a   Sakao, Kei ^a   Saito, Masazumi ^a   Honjo, Kuniaki ^a   Imanishi, Jiro ^a   Kubo, Toshikazu ^a  

^a KYOTO PREFECTURAL UNIVERSITY OF MEDICINE   (Japan)

Author keywords

Apoptosis; Chondrocyte; Glutathione; N acetylcysteine; Osteoarthritis

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; ACETYLCYSTEINE; BUTHIONINE SULFOXIMINE; CASPASE 3; GLUTATHIONE SYNTHASE; HOE 33342; NITRIC OXIDE; NITROPRUSSIDE SODIUM; PROTEIN P53; REACTIVE OXYGEN METABOLITE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIOXIDANT ACTIVITY; APOPTOSIS; ARTICLE; ARTICULAR CARTILAGE; BIOASSAY; CARTILAGE CELL; CARTILAGE DEGENERATION; CELL VIABILITY; CONTROLLED STUDY; ENZYME ACTIVATION; EXPERIMENTAL MODEL; MALE; NICK END LABELING; NONHUMAN; OSTEOARTHRITIS; PHOSPHORYLATION; PRIORITY JOURNAL; RABBIT; RAT; STAINING; UPREGULATION; WESTERN BLOTTING;

ACETYLCYSTEINE; ANIMALS; APOPTOSIS; CARTILAGE, ARTICULAR; CASPASE 3; CHONDROCYTES; DISEASE MODELS, ANIMAL; FREE RADICAL SCAVENGERS; GLUTATHIONE; INJECTIONS, INTRA-ARTICULAR; MALE; NITRIC OXIDE; OSTEOARTHRITIS; RABBITS; RATS; REACTIVE OXYGEN SPECIES; TUMOR SUPPRESSOR PROTEIN P53; UP-REGULATION;

EID: 73849152727     PISSN: 07360266     EISSN: None     Source Type: Journal    
DOI: 10.1002/jor.20976     Document Type: Article

References (39)

1. Listrat V, Ayral X, Patarnello F, et al. 1997. Arthroscopic evaluation of potential structure modifying activity of hyaluronan (Hyalgan) in osteoarthritis of the knee. Osteoarthritis Cartilage 5:153-160.
2. Buckwalter JA, Saltzman C, Brown T. 2004. The impact of osteoarthritis: implications for research. Clin Orthop Relat Res 427:S6-S15.
3. Reboul P, Pelletier JP, Tardif G, et al. 1996. The new collagenase, collagenase-3, is expressed and synthesized by human chondrocytes but not by synoviocytes. A role in osteoarthritis. J Clin Invest 97:2011-2019.
4. Blanco FJ, Guitian R, Vazquez-Martul E, et al. 1998. Osteoarthritis chondrocytes die by apoptosis. A possible pathway for osteoarthritis pathology. Arthritis Rheum 41:284-289.
5. Diaz-Gallego L, Prieto JG, Coronel P, et al. 2005. Apoptosis and nitric oxide in an experimental model of osteoarthritis in rabbit after hyaluronic acid treatment. J Orthop Res 23:1370-1376.
6. Wu GJ, Chen TG, Chang HC, et al. 2007. Nitric oxide from both exogenous and endogenous sources activates mitochondria-dependent events and induces insults to human chondrocytes. J Cell Biochem 101:1520-1531.
7. Pfeiffer S, Gorren AC, Schmidt K, et al. 1997. Metabolic fate of peroxynitrite in aqueous solution. Reaction with nitric oxide and pH-dependent decomposition to nitrite and oxygen in a 2:1 stoichiometry. J Biol Chem 272:3465-3470.
8. Lo YY, Conquer JA, Grinstein S, et al. 1998. Interleukin-1 beta induction of c-fos and collagenase expression in articular chondrocytes: involvement of reactive oxygen species. J Cell Biochem 69:19-29.
9. Stoop R, Buma P, van der Kraan PM, et al. 2000. Differences in type II collagen degradation between peripheral and central cartilage of rat stifle joints after cranial cruciate ligament transection. Arthritis Rheum 43:2121-2131.
10. Schreck R, Rieber P, Baeuerle PA. 1991. Reactive oxygen intermediates as apparently widely used messengers in the activation of the NF-kappa B transcription factor and HIV-1. EMBO J 10:2247-2258.
11. Reed DJ. 1990. Glutathione: toxicological implications. Annu Rev Pharmacol Toxicol 30:603-631.
12. Carlo MD Jr, Loeser RF. 2003. Increased oxidative stress with aging reduces chondrocyte survival: correlation with intracellular glutathione levels. Arthritis Rheum 48:3419-3430.
13. Dobashi K, Singh I, Orak JK, et al. 2002. Combination therapy of N-acetylcysteine, sodium nitroprusside and phosphoramidon attenuates ischemia-reperfusion injury in rat kidney. Mol Cell Biochem 240:9-17.
14. Smilkstein MJ, Bronstein AC, Linden C, et al. 1991. Acetaminophen overdose: a 48-hour intravenous N-acetylcysteine treatment protocol. Ann Emerg Med 20:1058-1063.
15. Tonomura H, Takahashi KA, Mazda O, et al. 2006. Glutamine protects articular chondrocytes from heat stress and NOinduced apoptosis with HSP70 expression. Osteoarthritis Cartilage 14:545-553.
16. Sah RL, Yang AS, Chen AC, et al. 1997. Physical properties of rabbit articular cartilage after transection of the anterior cruciate ligament. J Orthop Res 15:197-203.
17. Cake MA, Read RA, Guillou B, et al. 2000. Modification of articular cartilage and subchondral bone pathology in an ovine meniscectomy model of osteoarthritis by avocado and soya unsaponifiables (ASU). Osteoarthritis Cartilage 8:404-411.
18. Khan IM, Gilbert SJ, Caterson B, et al. 2008. Oxidative stress induces expression of osteoarthritis markers procollagen IIA and 3B3(-) in adult bovine articular cartilage. Osteoarthritis Cartilage 16:698-707.
19. Kim SJ, Ju JW, Oh CD, et al. 2002. ERK-1/2 and p38 kinase oppositely regulate nitric oxide-induced apoptosis of chondrocytes in association with p53, caspase-3, and differentiation status. J Biol Chem 277:1332-1339.
20. Quintero M, Mitrovic DR, Stankovic MA, et al. 1984. [Cellular aspects of the aging of the articular cartilage. II. Condylar cartilage with fissured surface taken from normal and arthritic knees]. Rev Rhum Mal Osteoartic 51:445-449.
21. Loeser RF, Carlson CS, Del Carlo M, et al. 2002. Detection of nitrotyrosine in aging and osteoarthritic cartilage: correlation of oxidative damage with the presence of interleukin-1beta and with chondrocyte resistance to insulin-like growth factor 1. Arthritis Rheum 46:2349-2357.
22. Kuhn K, Hashimoto S, Lotz M. 2000. IL-1 beta protects human chondrocytes from CD95-induced apoptosis. J Immunol 164:2233-2239.
23. Terauchi R, Takahashi KA, Arai Y, et al. 2003. Hsp70 prevents nitric oxide-induced apoptosis in articular chondrocytes. Arthritis Rheum 48:1562-1568.
24. Whyte IM, Francis B, Dawson AH. 2007. Safety and efficacy of intravenous N-acetylcysteine for acetaminophen overdose: analysis of the Hunter Area Toxicology Service (HATS) database. Curr Med Res Opin 23:2359-2368.
25. Zuin R, Palamidese A, Negrin R, et al. 2005. High-dose N-acetylcysteine in patients with exacerbations of chronic obstructive pulmonary disease. Clin Drug Investig 25:401-408.
26. Clancy RM, Abramson SB, Kohne C, et al. 1997. Nitric oxide attenuates cellular hexose monophosphate shunt response to oxidants in articular chondrocytes and acts to promote oxidant injury. J Cell Physiol 172:183-191.
27. Del Carlo M Jr, Loeser RF. 2002. Nitric oxide-mediated chondrocyte cell death requires the generation of additional reactive oxygen species. Arthritis Rheum 46:394-403.
28. Islam N, Haqqi TM, Jepsen KJ, et al. 2002. Hydrostatic pressure induces apoptosis in human chondrocytes from osteoarthritic cartilage through up-regulation of tumor necrosis factor-alpha, inducible nitric oxide synthase, p53, c-myc, and bax-alpha, and suppression of bcl-2. J Cell Biochem 87:266-278.
29. Haupt Y, Maya R, Kazaz A, et al. 1997. Mdm2 promotes the rapid degradation of p53. Nature 387:296-299.
30. Bode AM, Dong Z. 2004. Post-translational modification of p53 in tumorigenesis. Nat Rev Cancer 4:793-805.
31. Lake AN, Bedford MT. 2007. Protein methylation and DNA repair. Mutat Res 618:91-101.
32. Ito A, Lai CH, Zhao X, et al. 2001. p300/CBP-mediated p53 acetylation is commonly induced by p53-activating agents and inhibited by MDM2. EMBO J 20:1331-1340.
33. Bragado P, Armesilla A, Silva A, et al. 2007. Apoptosis by cisplatin requires p53 mediated p38alpha MAPK activation through ROS generation. Apoptosis 12:1733-1742.
34. Hashimoto S, Takahashi K, Amiel D, et al. 1998. Chondrocyte apoptosis and nitric oxide production during experimentally induced osteoarthritis. Arthritis Rheum 41:1266-1274.
35. Castro RR, Cunha FQ, Silva FS Jr, et al. 2006. A quantitative approach to measure joint pain in experimental osteoarthritis - evidence of a role for nitric oxide. Osteoarthritis Cartilage 14:769-776.
36. D'Lima D, Hermida J, Hashimoto S, et al. 2006. Caspase inhibitors reduce severity of cartilage lesions in experimental osteoarthritis. Arthritis Rheum 54:1814-1821.
37. Sylvester J, Liacini A, Li WQ, et al. 2004. Interleukin-17 signal transduction pathways implicated in inducing matrix metalloproteinase-3, -13 and aggrecanase-1 genes in articular chondrocytes. Cell Signal 16:469-476.
38. Homandberg GA, Hui F, Wen C. 1996. Fibronectin fragment mediated cartilage chondrolysis. I. Suppression by anti-oxidants. Biochim Biophys Acta 1317:134-142.
39. Inoue A, Takahashi KA, Arai Y, et al. 2006. The therapeutic effects of basic fibroblast growth factor contained in gelatin hydrogel microspheres on experimental osteoarthritis in the rabbit knee. Arthritis Rheum 54:264-270.