Digital screening methodology for the directed evolution of transglycosidases

Protein Engineering, Design and Selection

Volumn 22, Issue 1, 2009, Pages 37-44

  Koné, Fankroma M T ^a   Le Béchec, Mickaël ^a   Sine, Jean Pierre ^a   Dion, Michel ^a   Tellier, Charles ^a  

^a CNRS   (France)

Author keywords

Digital imaging; Directed evolution; High throughput screening; Transglycosidases

Indexed keywords

ACCEPTOR SUBSTRATES; DIGITAL IMAGING; DIRECTED EVOLUTION; ESCHERICHIA COLI CELLS; GLYCOCONJUGATES; GLYCOSIDASE; GLYCOSIDASES; GLYCOSYLTRANSFERASES; GLYCOSYNTHASES; HIGH-THROUGHPUT SCREENING; HYDROLYSIS ACTIVITIES; REGIO ISOMERS; SCREENING SYSTEMS; SYNTHESIS OF; TRANSFER ACTIVITIES; TRANSGLYCOSIDASES;

CARBOHYDRATES; ESCHERICHIA COLI; POLYSACCHARIDES; SUGAR (SUCROSE); SUGARS;

BUBBLES (IN FLUIDS);

BETA GLYCOSIDASE; GALACTOSIDASE; GLYCOSIDASE; TRANSGALACTOSIDASE; TRANSGLYCOSIDASE; UNCLASSIFIED DRUG;

ARTICLE; DIGITAL IMAGING; ENZYME ACTIVITY; ESCHERICHIA COLI; HYDROLYSIS; KINETICS; MOLECULAR EVOLUTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; THERMUS THERMOPHILUS; WILD TYPE;

CATALYTIC DOMAIN; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; GLYCOSIDE HYDROLASES; HYDROLYSIS; KINETICS; MULTIENZYME COMPLEXES; MUTATION; PLASMIDS; PROTEIN ENGINEERING; THERMUS THERMOPHILUS; TRANSFERASES;

ESCHERICHIA COLI; THERMUS THERMOPHILUS;

EID: 58149101116     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzn065     Document Type: Article

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