Purity, cell viability, expression of GFAP and bystin in astrocytes cultured by different procedures

Journal of Cellular Biochemistry

Volumn 109, Issue 1, 2010, Pages 30-37

  Du, Fang ^a,b   Qian, Zhong Ming ^a,c   Zhu, Li ^a,c   Wu, Xiao Mei ^a   Qian, Christopher ^b   Chan, Robbie ^c   Ke, Ya ^b  

^a NANTONG UNIVERSITY   (China)

^b and Hong Kong Branch of CAS Center for Excellence in Animal Evolution and Genetics   (Hong Kong)

^c HONG KONG POLYTECHNIC UNIVERSITY   (Hong Kong)

Author keywords

Astrocytes; Bystin; Glial fibrillary acidic protein (GFAP); Ischemia reperfusion; Primary culture; Reactive astrocytes; Shaken once culture; Shaken twice culture; Subculture

Indexed keywords

BYSTIN; GLIAL FIBRILLARY ACIDIC PROTEIN; PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; ASSAY; ASTROCYTE; CELL VIABILITY; CONTROLLED STUDY; CULTURE TECHNIQUE; EMBRYO; IMMUNOCYTOCHEMISTRY; ISCHEMIA; METABOLIC STRESS; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; STIMULATION; WESTERN BLOTTING;

ANIMALS; ANIMALS, NEWBORN; ASTROCYTES; BLOTTING, WESTERN; CELL ADHESION MOLECULES; CELL CULTURE TECHNIQUES; CELL SURVIVAL; CELLS, CULTURED; GLIAL FIBRILLARY ACIDIC PROTEIN; IMMUNOHISTOCHEMISTRY; MICE; MICE, INBRED ICR;

EID: 73849137656     PISSN: 07302312     EISSN: 10974644     Source Type: Journal    
DOI: 10.1002/jcb.22375     Document Type: Article

References (29)

1. Abbott NJ, Ronnback L, Hansson E. 2006. Astrocyte-endothelial interactions at the blood-brain barrier. Nat Rev Neurosci 7:41-53.
2. Araque A, Perea G. 2004. Glial modulation of synaptic transmission in culture. Glia 47:241-248.
3. Booher J, Sensenbrenner M. 1972. Growth and cultivation of dissociated neurons and glial cells from embryonic chick, rat and human brain in flask cultures. Neurobiology 2:97-105.
4. Darlington CL. 2005. Astrocytes as targets for neuroprotective drugs. Curr Opin Invest Drugs 6:700-703.
5. Du F, Zhu L, Qian ZM, Wu XM, Yung WH, Xu WY, Ke Y. 2008. Expression of bystin in reactive astrocytes induced by ischemia/reperfusion and chemical hypoxia in vitro. Biochim Biophys Acta 1782:658-663.
6. Eng LF, Ghirnikar RS, Lee YL. 2000. Glial fibrillary acidic protein: GFAP-thirty-one years (1969-2000). Neurochem Res 25:1439-1451.
7. Frangakis MV, Kimelberg HK. 1984. Dissociation of neonatal rat brain by dispase for preparation of primary astrocyte cultures. Neurochem Res 9(12): 1689-1698.
8. Gabryel B, Adamczyk J, Huzarska M, Pudełko A, Trzeciak HI. 2002. Aniracetam attenuates apoptosis of astrocytes aubjected to simulated ischemia in vitro. NeuroToxicol 23:385-395.
9. He W, Qian ZM, Zhu L, Qian C, Du F, Yung WH, Ke Y. 2008. Ginkgolides mimic the effects of hypoxic preconditioning to protect C6 cells against ischemic injury by up-regulation of HIF-1α and EPO. Int J Biochem Mol Biol 40(4): 651-662.
10. Innocenti B, Parpura V, Haydon PG. 2000. Imaging extracellular waves of glutamate during calcium signaling in cultured astrocytes. J Neurosci 20(5):1800-1808.
11. Koehler RC, Gebremedhin D, Harder DR. 2006. Role of astrocytes in cerebrovascular regulation. J Appl Physiol 100:307-317.
12. McCarthy KD, de Vellis J. 1980. Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue. J Cell Biol 85(3): 890-902.
13. Murray CE, Jennings RB, Reimer KA. 1986. Preconditioning with ischaemia: A delay of lethal cell injury in ischaemic myocardium. Circulation 74:1124-1136.
14. Pekny M, Nilsson M. 2005. Astrocyte activation and reactive gliosis. Glia 50(4): 427-434.
15. Pellerin L. 2005. How astrocytes feed hungry neurons. Mol Neurobiol 32:59-72.
16. Qian ZM, To Y, Tang PL, Feng YM. 1999. Transferrin receptor on the plasma membrane of cultured rat astrocytes. Exp Brain Res 129(3): 473-476.
17. Qian ZM, Liao QK, To Y, Ke Y, Tsoi YK, Wang GF, Ho KP. 2000. Transferrin-bound and transferrin-free iron uptake by the cultured brain astrocytes. Cell Mol Biol 46(3): 541-548.
18. Rzigalinski BA, Liang S, McKinney JS, Willoughby KA, Ellis EF. 1997. Effect of Ca2+on in vitro astrocyte injury. J Neurochem 68:289-296.
19. Saura J. 2007. Microglial cells in astroglial cultures: A cautionary note. J Neuroinflam 4:26. 10.1186/1742-2094-4-26.
20. Sheng J, Yang S, Xu L, Wu C, Wu X, Li A, Yu Y, Ni H, Fukuda M, Zhou J. 2004. Bystin as a novel marker for reactive astrocytes in the adult rat brain following injury. Eur J Neurosci 20(4): 873-884.
21. Sofroniew MV. 2005. Reactive astrocytes in neural repair and protection. Neuroscientist 11:400-407.
22. Suzuki N, Zara J, Sato T, Ong E, Bakhiet N, Oshima RG, Watson K, Fukuda MN. 1998. A cytoplasmic protein, bystin, interacts with trophinin, tastin, and cytokeratin and may be involved in trophinin-mediated cell adhesion between trophoblast and endometrial epithelial cells. Proc Natl Acad Sci USA 95:5027-5032.
23. Suzuki N, Nakayama J, Shih IM, Aoki D, Nozawa S, Fukuda MN. 1999. Expression of trophinin, tastin, and bystin by trophoblast and endometrial cells in human placenta. Biol Reprod 60:621-627.
24. Swanson RA, Ying W, Kauppinen TM. 2004. Astrocyte influences on ischemic neuronal death. Curr Mol Med 4(2): 193-205.
25. Tabernero A, Orfao A, Medina JM. 1996. Astrocyte differentiation in primary culture followed by flow cytometry. Neurosci Res 24(2): 131-138.
26. Verkhratsky A, Toescu EC. 2006. Neuronal-glial networks as substrate for CNS integration. J Cell Mol Med 10(4): 826-836.
27. Wu VW, Schwartz JP. 1998. Cell culture models for reactive gliosis: New perspectives. J Neurosci Res 51:675-681.
28. Zhu L, Ke Y, Wu X, Yang L, Lu Y, Qian ZM. 2008. Ginkgolides protect PC12 cells against hypoxia-induced injury by p42/p44 MAPK pathway-dependent upregulation of HIF-1a expression and HIF-1DNA-binding activity. J Cell Biochem 103(2): 564-575.
29. Zwain IH, Yen SSC. 1999. Neurosteroidogenesis in astrocytes, oligodendrocytes, and neurons of cerebral cortex of rat brain. Endocrinology 140:3843-3852.