Large volume injection of hexane solutions in RPLC/UV to enhance on sensitivity of the assay of ginkgolic acids in gingko biloba standardized extracts

Journal of Liquid Chromatography and Related Technologies

Volumn 33, Issue 1, 2010, Pages 133-149

  Udrescu, Stefan ^a   Sora, Iuliana Daniela ^a   David, Victor ^b   Medvedovici, Andrei ^b  

^a LaborMed Pharma SA   (Romania)

^b UNIVERSITY OF BUCHAREST   (Romania)

Author keywords

Direct injection of n hexane solutions; Ginkgolic acids; Improved sensitivity; Liquid liquid extraction; RPLC UV; Validation

Indexed keywords

ANALYTES; CHROMATOGRAPHIC COLUMN; CONCENTRATION FACTORS; DETECTION SYSTEM; DIRECT INJECTION; EUROPEAN PHARMACOPOEIAS; GINGKO BILOBA; GINKGO BILOBA; GINKGOLIC ACIDS; GRADIENT ELUTION; HEXANE SOLUTIONS; IN-SITU; LARGE VOLUME INJECTION; LIMIT OF QUANTITATIONS; LINEARITY RANGE; LIQUID-LIQUID EXTRACTION; N-HEXANE; OPERATING GUIDELINES; ORGANIC LAYERS; QUANTITATION LIMITS; SAMPLE INJECTION; SAMPLE PREPARATION PROCEDURE; SAMPLE SOLVENT; TARGET COMPOUND; UV DETECTION;

HEXANE; LIQUIDS;

SOLVENT EXTRACTION;

GINKGO BILOBA EXTRACT; HEXANE; METHANOL; WATER;

ACCURACY; ARTICLE; CHEMICAL PROCEDURES; COLUMN CHROMATOGRAPHY; ELECTROSPRAY MASS SPECTROMETRY; GINKGO BILOBA; NONHUMAN; REVERSED PHASE LIQUID CHROMATOGRAPHY; SENSITIVITY ANALYSIS; ULTRAVIOLET RADIATION;

GINKGO BILOBA;

EID: 73849129195     PISSN: 10826076     EISSN: 1520572X     Source Type: Journal    
DOI: 10.1080/10826070903430514     Document Type: Article

References (25)

1. Zarnowska, E.D.; Zarnowski, R.; Kozubek, A. Alkylresorcinols in the fruit pulp and leaves of Ginkgo biloba L. Z. Natur-forsch. 2000, 55c, 881-885.
2. Irie, J.; Murata, M.; Homma, S. Glycerol-3-phosphate dehydrogenase inhibitors, anacardic acids, from Ginkgo biloba. Biosci. Biotech. Biochem. J. 1996, 60, 240-243.
3. Itokawa, H.; Totsuka, N.; Nakahara, K.; Takeya, K.; Lepoittevin, J.P.; Asakawa, Y. Antitumor principles from Ginkgo biloba L. Chem. Pharm. Bull. 1987, 35, 3016-3020.
4. Itokawa, H.; Totsuka, N.; Nakahara, K.; Maezuru, M.; Takeya, K.; Kondo, M.; Inamatsu, M. Morita, H. A quantitative structure-activity relationship for antitumor activity of long-chain phenols from Ginkgo biloba L. Chem. Pharm. Bull. 1989, 37, 1619-1621.
5. Ahlemeyer, B.; Selke, D.; Schaper, C.; Klumpp, S.; Krieglstein, J. Ginkgolic acids induce neuronal death and activate protein phosphatase type-2C. Eur. J. Pharmacol. 2001, 430, 1-7.
6. Westendorf, J.; Regan, J. Induction of DNA-strand breaks in primary rat hepatocytes by ginkgolic acids. Pharmazie. 2000, 55, 864-865.
7. Koch, E.; Jaggy, H.; Chatterjee, S.S. Evidence for immunotoxic effects of crude Ginkgo biloba L. leaf extracts using the popliteal lymph node assay in the mouse. Int. J. Immunopharmacol. 2000, 22, 229-236.
8. Jaggy, H.; Koch, E. Chemistry and biology of alkylphenols from Ginkgo biloba L. Pharmazie. 1997, 52, 735-738.
9. Blumenthal, M. German Government Limits Ginkgolic Acid Levels. HerbalGram. 1997, 41, 29-31.
10. European Pharmacopoeia, Council of Europe, Strasbourg, France, VIth Edition, monograph 04=2008:1827, 3461-3463.
11. van Beek, T.A.; Montoro, P. Chemical analysis and quality control of Ginkgo biloba leaves, extracts, and phytopharmaceuticals. J. Chromatogr. A 2009, 1216, 2002-2032.
12. Fuzatti, N.; Pace, R.; Villa, F. A simple HPLC-UV method for the assay of ginkgolic acids in Ginkgo biloba extracts. Fitoterapia. 2003, 74, 247-256.
13. van Beek, T.A.; Wintermans, M.S. Preparative isolation and dual column high-performance liquid chromatography of ginkgolic acids from Ginkgo biloba. J. Chromatogr. A 2001, 930, 109-117.
14. He, J.; Xie, B. Reversed-phase argentation high-performance liquid chromatography in phytochemical analysis of ginkgolic acids in leaves from Gingko biloba L. J. Chromatogr. A 2002, 943, 303-309.
15. Ndjoko, K.; Wolfender, J.L.; Hostettmann, K. Determination of trace amounts of ginkgolic acids in Ginkgo biloba L. leaf extracts and phytopharmaceuticals by liquid chromatography-electrospray mass spectrometry. J. Chromatogr. B. 2000, 744, 249-255.
16. Camponovo, F.; Wolfender, J.L.; Maillard, M.P.; Potterat, O.; Hostettmann, K. Evaporative light scattering and thermospray mass spectrometry: two alternative methods for detection and quantitative liquid chromatographic determination of ginkgolides and bilobalide in Ginkgo biloba leaf extracts and phytopharmaceuticals. Phytochem. Anal. 1995, 6, 141-148.
17. Medvedovici, A.; David, V.; David, V.; Georgita, C. Retention phenomena induced by large volume injection of solvents non-miscible with the mobile phase in reversed-phase liquid chromatography. J. Liq. Chromatogr. Rel. Technol. 2007, 30, 199-213.
18. Udrescu, S.; Medvedovici, A.; David, V. Effect of large volume injection of hydrophobic solvents on the retention of less hydrophobic pharmaceutical solutes in RP-LC. J. Sep. Sci. 2008, 31, 2939-2945.
19. David, V.; Barcutean, C.; Georgita, C.; Medvedovici, A. Non-miscible solvent large volumeinjection-HPLC=DAD method for determination of butylated hydroxyanisole in lovastatin and simvastatin pharmaceutical formulations. Rev. Roum. Chim. 2006, 51, 447-453.
20. Loeser, E.; Babiak, S.; Drumm, P. Water-immiscible solvents as diluents in reversed-phase liquid chromatography. J. Chromatogr. A 2009, 1216, 3409-3412.
21. Note for guidance on validation of analytical procedures: methodology, (CPMP=ICH=281=95) ICH topic Q2B, ICH Technical Coordination, London, UK (1996).
22. Note for guidance on validation of analytical procedures: text and methodology, (CPMP=ICH= 381=95) ICH topic Q2 R1, ICH Technical Coordination, London, UK (1996).
23. Van Beek, T.A. Chemical analysis of Ginkgo biloba leaves and extracts. J. Chromatogr. A. 2002, 967, 21-55.
24. Leo, A.J. Calculating log Poct from structures. Chem. Rev. 1993, 93, 1281-1306.
25. Liteanu, C.; Rica, I. Statistical theory and methodology of trace analysis, chapter 6-detection theory of analytical signals. Ellis Horwood, Chichester 1980, 165-175.