Deletion of penicillin-binding protein 5 (PBP5) sensitises Escherichia coli cells to β-lactam agents

International Journal of Antimicrobial Agents

Volumn 35, Issue 3, 2010, Pages 244-249

  Sarkar, Sujoy K ^a   Chowdhury, Chiranjit ^a   Ghosh, Anindya S ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY   (India)

Author keywords

Lactam sensitivity; Escherichia coli; Penicillin binding protein

Indexed keywords

AMOXICILLIN; AMPICILLIN; BACTERIAL PROTEIN; BETA LACTAM ANTIBIOTIC; CEFACLOR; CEFADROXIL; CEFALEXIN; CEFALOTIN; CEPHALOSPORIN DERIVATIVE; CHLORAMPHENICOL; KANAMYCIN; NITROCEFIN; O ANTIGEN; PENICILLIN BINDING PROTEIN 5; PENICILLIN DERIVATIVE; PENICILLIN G; PIPERACILLIN; PROTEIN DACA; TETRACYCLINE; UNCLASSIFIED DRUG;

ANTIBIOTIC SENSITIVITY; ANTIGEN EXPRESSION; ARTICLE; BACTERIAL CELL; BACTERIAL STRAIN; CELL SHAPE; CELL SURVIVAL; CONTROLLED STUDY; ESCHERICHIA COLI; ESCHERICHIA COLI K 12; HAEMOPHILUS INFLUENZAE; MICROBIAL ACTIVITY; MINIMUM INHIBITORY CONCENTRATION; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; SALMONELLA ENTERICA; SALMONELLA TYPHIMURIUM; SEQUENCE HOMOLOGY; SEROTYPE; STRAIN DIFFERENCE; VIBRIO CHOLERAE;

ANTI-BACTERIAL AGENTS; BETA-LACTAMS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC COMPLEMENTATION TEST; HAEMOPHILUS INFLUENZAE; HUMANS; MICROBIAL SENSITIVITY TESTS; PENICILLIN-BINDING PROTEINS; SALMONELLA TYPHIMURIUM; SEQUENCE DELETION; VIBRIO CHOLERAE;

EID: 73549109625     PISSN: 09248579     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijantimicag.2009.11.004     Document Type: Article

References (34)

1. Denome S.A., Elf P.K., Henderson T.A., Nelson D.E., and Young K.D. Escherichia coli mutants lacking all possible combinations of eight penicillin binding proteins: viability, characteristics, and implications for peptidoglycan synthesis. J Bacteriol 181 (1999) 3981-3993
2. Nelson D.E., and Young K.D. Penicillin binding protein 5 affects cell diameter, contour, and morphology of Escherichia coli. J Bacteriol 182 (2000) 1714-1721
3. Nelson D.E., and Young K.D. Contributions of PBP5 and dd-carboxypeptidase penicillin binding proteins to maintenance of cell shape in Escherichia coli. J Bacteriol 183 (2001) 3055-3064
4. Dougherty T.J., Kennedy K., Kessler R.E., and Pucci M.J. Direct quantitation of the number of individual penicillin-binding proteins per cell in Escherichia coli. J Bacteriol 178 (1996) 6110-6115
5. Ghosh A.S., Chowdhury C., and Nelson D.E. Physiological functions of d-alanine carboxypeptidases in Escherichia coli. Trends Microbiol 16 (2008) 309-317
6. Holtje J.V. Growth of the stress-bearing and shape-maintaining murein sacculus of Escherichia coli. Microbiol Mol Biol Rev 62 (1998) 181-183
7. Spratt B.G. Hybrid penicillin-binding proteins in penicillin-resistant strains of Neisseria gonorrhoeae. Nature 332 (1988) 173-176
8. Dore M.P., Graham D.Y., and Sepulveda A.R. Different penicillin-binding protein profiles in amoxicillin-resistant Helicobacter pylori. Helicobacter 4 (1999) 154-161
9. Gerrits M.M., Godoy A.P., Kuipers E.J., Ribeiro M.L., Stoof J., Mendonca S., et al. Multiple mutations in or adjacent to the conserved penicillin-binding protein motifs of the penicillin-binding protein 1A confer amoxicillin resistance to Helicobacter pylori. Helicobacter 11 (2006) 181-187
10. Gotoh N., Nunomura K., and Nishino T. Resistance of Pseudomonas aeruginosa to cefsulodin: modification of penicillin-binding protein 3 and mapping of its chromosomal gene. J Antimicrob Chemother 25 (1990) 513-523
11. Ghosh A.S., Kar A.K., and Kundu M. Alterations in high molecular mass penicillin-binding protein 1 associated with β-lactam resistance in Shigella dysenteriae. Biochem Biophys Res Commun 248 (1998) 669-672
12. Ropp P.A., Hu M., Olesky M., and Nicholas R.A. Mutations in ponA, the gene encoding penicillin-binding protein 1, and a novel locus, penC, are required for high-level chromosomally mediated penicillin resistance in Neisseria gonorrhoeae. Antimicrob Agents Chemother 46 (2002) 769-777
13. Spratt B.G., Zhang Q.Y., Jones D.M., Hutchison A., Brannigan J.A., and Dowson C.G. Recruitment of a penicillin-binding protein gene from Neisseria flavescens during the emergence of penicillin resistance in Neisseria meningitidis. Proc Natl Acad Sci USA 86 (1989) 8988-8992
14. Moya B., Dotsch A., Juan C., Blazquez J., Zamorano L., Haussler S., et al. β-Lactam resistance response triggered by inactivation of a nonessential penicillin-binding protein. PLoS Pathog 5 (2009) e1000353
15. Sarkar S.K., and Ghosh A.S. Involvement of O8-antigen in altering β-lactam antibiotic susceptibilities in Escherichia coli. FEMS Microbiol Lett 282 (2008) 59-64
16. Nilsen T., Ghosh A.S., Goldberg M.B., and Young K.D. Branching sites and morphological abnormalities behave as ectopic poles in shape-defective Escherichia coli. Mol Microbiol 52 (2004) 1045-1054
17. Ghosh A.S., and Young K.D. Sequences near the active site in chimeric penicillin binding proteins 5 and 6 affect uniform morphology of Escherichia coli. J Bacteriol 185 (2003) 2178-2186
18. Kristensen C.S., Eberl L., Sanchez-Romero J.M., Givskov M., Molin S., and De Lorenzo V. Site-specific deletions of chromosomally located DNA segments with the multimer resolution system of broad-host-range plasmid RP4. J Bacteriol 177 (1995) 52-58
19. Zhao G., Meier T.I., Kahl S.D., Gee K.R., and Blaszczak L.C. Bocillin FL, a sensitive and commercially available reagent for detection of penicillin-binding proteins. Antimicrob Agents Chemother 43 (1999) 1124-1128
20. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. Seventeenth informational supplement. Document M100-S17. CLSI, Wayne, PA: CLSI; 2007.
21. Stubbs K.A., Balcewich M., Mark B.L., and Vocadlo D.J. Small molecule inhibitors of a glycoside hydrolase attenuate inducible AmpC-mediated β-lactam resistance. J Biol Chem 282 (2007) 21382-21391
22. Ghosh A.S., Kar A.K., and Kundu M. Impaired imipenem uptake associated with alterations in outer membrane proteins and lipopolysaccharides in imipenem-resistant Shigella dysenteriae. J Antimicrob Chemother 43 (1999) 195-201
23. Nicholas R.A., and Strominger J.L. Site-directed mutants of a soluble form of penicillin-binding protein 5 from Escherichia coli and their catalytic properties. J Biol Chem 263 (1988) 2034-2040
24. Ahamed J., and Kundu M. Molecular characterization of the SHV-11 β-lactamase of Shigella dysenteriae. Antimicrob Agents Chemother 43 (1999) 2081-2083
25. Babini G.S., Yuan M., and Livermore D.M. Interactions of β-lactamases with sanfetrinem (GV 104326) compared to those with imipenem and with oral β-lactams. Antimicrob Agents Chemother 42 (1998) 1168-1175
26. El-Gindy A., El Walily A.F., and Bedair M.F. First-derivative spectrophotometric and LC determination of cefuroxime and cefadroxil in urine. J Pharm Biomed Anal 23 (2000) 341-352
27. Bae I.K., Lee B.H., Hwang H.Y., Jeong S.H., Hong S.G., Chang C.L., et al. A novel ceftazidime-hydrolysing extended-spectrum β-lactamase, CTX-M-54, with a single amino acid substitution at position 167 in the omega loop. J Antimicrob Chemother 58 (2006) 315-319
28. O'Callaghan C.H., Morris A., Kirby S.M., and Shingler A.H. Novel method for detection of β-lactamases by using a chromogenic cephalosporin substrate. Antimicrob Agents Chemother 1 (1972) 283-288
29. Liu D., and Reeves P.R. Escherichia coli K12 regains its O antigen. Microbiology 140 (1994) 49-57
30. Pepper E.D., Farrell M.J., and Finkel S.E. Role of penicillin-binding protein 1b in competitive stationary-phase survival of Escherichia coli. FEMS Microbiol Lett 263 (2006) 61-67
31. Nishimura Y., Suzuki H., Hirota Y., and Park J.T. A mutant of Escherichia coli defective in penicillin-binding protein 5 and lacking carboxypeptidase 1A. J Bacteriol 143 (1980) 531-534
32. Davies C., White S.W., and Nicholas R.A. Crystal structure of a deacylation-defective mutant of penicillin-binding protein 5 at 2.3-Ǻ resolution. J Biol Chem 276 (2001) 616-623
33. Georgopapadakou N.H. Penicillin-binding proteins and bacterial resistance to β-lactams. Antimicrob Agents Chemother 37 (1993) 2045-2053
34. Santos J.M., Lobo M., Matos A.P., De Pedro M.A., and Arraiano C.M. The gene bolA regulates dacA (PBP5), dacC (PBP6) and ampC (AmpC), promoting normal morphology in Escherichia coli. Mol Microbiol 45 (2002) 1729-1740