Sequences near the active site in chimeric penicillin binding proteins 5 and 6 affect uniform morphology of Escherichia coli

Journal of Bacteriology

Volumn 185, Issue 7, 2003, Pages 2178-2186

  Ghosh, Anindya S ^a   Young, Kevin D ^a  

^a UNIVERSITY OF NORTH DAKOTA SCHOOL OF MEDICINE AND HEALTH SCIENCES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; BACTERIAL PROTEIN; HYBRID PROTEIN; PENICILLIN BINDING PROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL CELL; CARBOXY TERMINAL SEQUENCE; CELL STRUCTURE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ESCHERICHIA COLI; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FUNCTION; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; ASPARTIC ACID; BACTERIAL PROTEINS; BINDING SITES; CARRIER PROTEINS; ESCHERICHIA COLI; HEXOSYLTRANSFERASES; LYSINE; MOLECULAR SEQUENCE DATA; MURAMOYLPENTAPEPTIDE CARBOXYPEPTIDASE; MUTAGENESIS, SITE-DIRECTED; PENICILLIN-BINDING PROTEINS; PEPTIDYL TRANSFERASES; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; STRUCTURE-ACTIVITY RELATIONSHIP;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0037378572     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.185.7.2178-2186.2003     Document Type: Article

References (28)

1. Altschul, S. F., T. L. Madden, A. A. Schaffer, J. Zhang, Z. Zhang, W. Miller, and D. J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402.
2. Amanuma, H., and J. L. Strominger. 1980. Purification and properties of penicillin-binding proteins 5 and 6 from Escherichia coli membranes. J. Biol. Chem. 255:11173-11180.
3. Baquero, M.-R., M. Bouzon, J. C. Quintela, J. A. Ayala, and F. Moreno. 1996. dacD, an Escherichia coli gene encoding a novel penicillin-binding protein (PBP6b) with DD-carboxypeptidase activity. J. Bacteriol. 178:7106-7111.
4. Begg, K. J., A. Takasuga, D. H. Edwards, S. J. Dewar, B. G. Spratt, H. Adachi, T. Ohta, H. Matsuzawa, and W. D. Donachie. 1990. The balance between different peptidoglycan precursors determines whether Escherichia coli cells will elongate or divide. J. Bacteriol. 172:6697-6703.
5. Botta, G. A., and J. T. Park. 1981. Evidence for involvement of penicillin-binding protein 3 in murein synthesis during septation but not during cell elongation. J. Bacteriol. 145:333-340.
6. Broome-Smith, J. K., I. Ioannidis, A. Edelman, and B. G. Spratt. 1988. Nucleotide sequences of the penicillin-binding protein 5 and 6 genes of Escherichia coli. Nucleic Acids Res. 16:1617.
7. Chang, Y.-H., M. R. Labgold, and J. H. Richards. 1990. Altering enzymatic activity: recruitment of carboxypeptidase activity into an RTEM β-lactamase/penicillin-binding protein 5 chimera. Proc. Natl. Acad. Sci. USA 87:2823-2827.
8. Dabernat, H., C. Delmas, M. Seguy, R. Pelissier, G. Faucon, S. Bennamani, and C. Pasquier. 2002. Diversity of β-lactam resistance-conferring amino acid substitutions in penicillin-binding protein 3 of Haemophilus influenzae. Antimicrob. Agents Chemother. 46:2208-2218.
9. Davies, C., S. W. White, and R. A. Nicholas. 2001. Crystal structure of a deacylation-defective mutant of penicillin-binding protein 5 at 2.3-Å resolution. J. Biol. Chem. 276:616-623.
10. Denome, S. A., P. K. Elf, T. A. Henderson, D. E. Nelson, and K. D. Young. 1999. Escherichia coli mutants lacking all possible combinations of eight penicillin binding proteins: viability, characteristics, and implications for peptidoglycan synthesis. J. Bacteriol. 181:3981-3993.
11. de Pedro, M. A., K. D. Young, J.-V. Höltje, and H. Schwarz. 2003. Branching of Escherichia coli cells arises from multiple sites of inert peptidoglycan. J. Bacteriol. 185:1147-1152.
12. Ghuysen, J.-M. 1994. Molecular structures of penicillin-binding proteins and β-lactamases. Trends Microbiol. 2:372-380.
13. Goffin, C., and J.-M. Ghuysen. 1998. Multimodular penicillin-binding proteins: an enigmatic family of orthologs and paralogs. Microbiol. Mol. Biol. Rev. 62:1079-1093.
14. BAD promoter. J. Bacteriol. 177:4121-4130.
15. Hayashi, T., K. Makino, M. Ohnishi, K. Kurokawa, K. Ishii, K. Yodoyama, C.-G. Han, E. Ohtsubo, K. Nakayama, T. Murata, M. Tanaka, T. Tobe, T. Iida, H. Takami, T. Honda, C. Sasakawa, N. Ogasawara, T. Yasunaga, S. Kuhara, T. Shiba, M. Hattori, and H. Shinagawa. 2001. Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12. DNA Res. 8:11-22.
16. Henderson, T. A., P. M. Dombrosky, and K. D. Young. 1994. Artifactual processing of penicillin-binding proteins 7 and 1b by the Omp T protease of Escherichia coli. J. Bacteriol. 176:256-259.
17. Koch, A. L. 2000. Simulation of the conformation of the murein fabric: the oligoglycan, penta-muropeptide, and cross-linked nona-muropeptide. Arch. Microbiol. 174:429-439.
18. Malhotra, K. T., and R. A. Nicholas. 1992. Substitution of lysine 213 with arginine in penicillin-binding protein 5 of Escherichia coli abolishes D-alanine carboxypeptidase activity without affecting penicillin binding. J. Biol. Chem. 267:11386-11391.
19. Massova, I., and S. Mobashery. 1998. Kinship and diversification of bacterial penicillin-binding proteins and β-lactamases. Antimicrob. Agents Chemother. 42:1-17.
20. Meberg, B. M., F. C. Sailer, D. E. Nelson, and K. D. Young. 2001. Reconstruction of Escherichia coli mrcA (PBP 1a) mutants lacking multiple combinations of penicillin binding proteins. J. Bacteriol. 183:6148-6149.
21. Nelson, D. E., A. S. Ghosh, A. L. Paulson, and K. D. Young. 2002. Contribution of membrane-binding and enzymatic domains of penicillin binding protein 5 to maintenance of uniform cellular morphology of Escherichia coli. J. Bacteriol. 184:3630-3639.
22. Nelson, D. E., and K. D. Young. 2001. Contributions of PBP 5 and DD-carboxypeptidase penicillin binding proteins to maintenance of cell shape in Escherichia coli. J. Bacteriol. 183:3055-3064.
23. Nelson, D. E., and K. D. Young. 2000. Penicillin binding protein 5 affects cell diameter, contour, and morphology of Escherichia coli. J. Bacteriol. 182:1714-1721.
24. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
25. Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
26. Ubukata, K., Y. Shibasaki, K. Yamamoto, N. Chiba, K. Hasegawa, Y. Takeuchi, K. Sunakawa, M. Inoue, and M. Konno. 2001. Association of amino acid substitutions in penicillin-binding protein 3 with β-lactam resistance in β-lactamase-negative ampicillin-resistant Haemophilus influenzae. Antimicrob. Agents Chemother. 45:1693-1699.
27. van der Linden, M. P., L. de Haan, M. A. Hoyer, and W. Keck. 1992. Possible role of Escherichia coli penicillin-binding protein 6 in stabilization of stationary-phase peptidoglycan. J. Bacteriol. 174:7572-7578.
28. van der Linden, M. P. G., L. de Haan, O. Dideberg, and W. Keck. 1994. Site-directed mutagenesis of proposed active-site residues of penicillin-binding protein 5 from Escherichia coli. Biochem. J. 303:357-362.