Enzymic cleavage of fusion protein using immobilized urokinase covalently conjugated to glyoxyl-agarose

Biotechnology and Applied Biochemistry

Volumn 37, Issue 2, 2003, Pages 149-155

  Suh, Chang Woo ^a   Choi, Gang Sun ^b   Lee, Eun Kyu ^a  

^a Hanyang University   (South Korea)

^b Fermentation Research Laboratory   (South Korea)

Author keywords

Amine coupling; Glyoxyl Sepharose; Human growth hormone; Immobilized protease; Selective precipitation

Indexed keywords

ENZYMES; HORMONES; PH EFFECTS; THERMODYNAMIC STABILITY;

HUMAN GROWTH HORMONES (HGH);

BIOCHEMISTRY;

AGAROSE; ALDEHYDE; AMINE; GLUTATHIONE TRANSFERASE; GLYCIDOL; HUMAN GROWTH HORMONE; HYBRID PROTEIN; SEPHAROSE; TETRAPEPTIDE; UROKINASE;

ARTICLE; CONJUGATION; ENZYME ACTIVITY; IMMOBILIZATION; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; THERMOSTABILITY;

AFFINITY LABELS; CATALYSIS; ENZYME ACTIVATION; ENZYME STABILITY; ENZYMES, IMMOBILIZED; GLUTATHIONE TRANSFERASE; HUMAN GROWTH HORMONE; HUMANS; MEMBRANES, ARTIFICIAL; RECOMBINANT FUSION PROTEINS; SEPHAROSE; URINARY PLASMINOGEN ACTIVATOR;

EID: 0346034544     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20020049     Document Type: Article

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