Effects of organic nitrogen and carbon sources on mycelial growth and polysaccharides production and their optimization in the submerged culture of Grifola umbellate, a Chinese medicinal herb

African Journal of Biotechnology

Volumn 8, Issue 20, 2009, Pages 5208-5214

  Yuan, Zhou ^a   Shao, Hong Bo ^a,b,c,d   Chen, De Yu ^a  

^a NORTHWEST A F UNIVERSITY   (China)

^b BINZHOU UNIVERSITY   (China)

^c YANTAI INSTITUTE OF COASTAL ZONE RESEARCH   (China)

^d QINGDAO UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Carbon source; Chinese medicinal herb. polysaccharides; Grifola umbellate; Organic nitrogen source; Orthogonal design; Submerged culture

Indexed keywords

EXOPOLYSACCHARIDE; FRUCTOSE; GLUCOSE; ORGANIC NITROGEN; POLYSACCHARIDE; SUCROSE;

ARTICLE; CARBOHYDRATE SYNTHESIS; CARBON SOURCE; CHINESE HERB; CULTURE MEDIUM; DRY WEIGHT; EXPERIMENTAL STUDY; GRIFOLA; GRIFOLA UMBELLATE; MYCELIAL GROWTH; NONHUMAN; SOYBEAN; SUBMERGED CELL CULTURE;

BASIDIOMYCOTA; FUNGI; GLYCINE MAX; GRIFOLA;

EID: 73349138879     PISSN: None     EISSN: 16845315     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Miao MS, Li ZG (2000). Quality Control Techniques of Modern Chinese Traditional Medicine. Beijing: People's Medical Publishing House, pp. 369-382.
2. Ji YB (2005). Chemistry and Pharmacology of Chinese Traditional Medicine Polysaccharide. Bejing: People's Medical Publishing House, pp. 946-947.
3. Xu GB, Fu WJ, Zhao XK (2003). Advances in studies on Polyporus umbellatus in China. J. Fungal Res. 158-161.
4. Chen BQ, Deng BW, Zhang J (2002). Study on selection of culture media for Polyporus umbellate, Amino Acids Biotic Resour. 24: 23-24.
5. Chen RS, Li JB (2004). Anticancer effect of Polyporus umbellatus morel water extract, J. Chinese Materia Med. 29: 481-482.
6. H, Liu L, Ning CX (2005). The synergistic effect observation of interferon, polysaccharides of Polyporus umbellatus and hepatitis B vaccine on treating chronic hepatitis B, Mod. Preventive Med. 32: p. 537.
7. Zhang WD, Lv C, Zhao HY, Chen SL, Yang DJ, Lv AP (2007). Influence of the polysaccharides of Radix Ginseng and Polyporus umbellatus on CIA rats intestinal mucosal immunocyte function, Chinese J. Cell. Mol. Immunol. 23: 867-871.
8. Xu ZR (1998). Regression analysis and experimental design. Beijing: China Agriculture Press, pp. 140-141.
9. Xu GB, Li TY, Li YR, Liang YJ, Jin QR (2004). Effect of shallow liquid culture on mycelia of Polypor us umbellatus and its polysaccharides content, Journal of Jilin Agricultural University, 26: 287-290.
10. Dai RQ, Qin P, Liu WJ (1979). Study on Polyporus umbellatus mycelial growth in liquid culture and the antitumor effect. J. Tradit. Chin. Med. 2: 19-22.
11. Zhou XY, Gu SM, Zhang WY, Xu ZS (2001). Fermentative production of mycelia by Polyporus umbellatus (Pers) Frie. Ind. Microbiol. 31: 1-4.
12. TY, Tian GY, Xu GB (2007). Effect of polysaccharides from mycelial of Grifula umbellateon mice's immunological level, Chin. J. Vet. Surg. 27: 88-94.
13. Wang LL, Wu HY, Luo DF (2000). The pharmacological effects and clinical application of Polyporus umbellatus. China Pharmaceut. 9: 58-59.
14. Gu FH, Yin H, Ma J (2001). The influences of different carbon and nitrogen sources on mycelial growth and extracellular polysaccharide yields of Polyporus umbellatus. Journal of Northwest University (Natural Science Edition), 31: 437-440.
15. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956). Colorimetric method for determination of sugars and related substances. Analyt. Chem. 28: 350-356.
16. Cai WC, Yuan HJ (1982). Common chemoanalytic methods of biosubstances. Bejing: Science Press, pp. 8-9, 59-60.
17. Hromádková Z, Ebringerová A, Valachovič P (1999). Comparison of classical and ultrasound-assisted extraction of polysaccharides from Salvia officinalis L, Ultrason. Sonochem. 50: 163-168.
18. Shih IL, Pan K, Hsieh C (2006). Influence of nutritional components and oxygen supply on the mycelial growth and bioactive metabolites production in submerged culture of Antrodia cinnamomea, Process Biochem. 41: 1129-1135.
19. Wu J, Ding ZY, Zhang KC (2006). Improvement of exopolysaccharide production by macro-fungus Auricularia auricula in submerged culture, Enzyme Microb. Technol. 39: 743-749.
20. Hwang HJ, Kim SW, Xu CP, Choi JW, Yun JW (2003). Production and molecular characteristics of four groups of exopolysaccharides from submerged culture of Phellinus gilvus, J. Appl. Microbiol. 94: 708-719.
21. Wei C, Zhao Z, Shi FC, Yang QL (2008). Optimization for the production of exopolysaccharide from Fomes fomentarius in submerged culture and its antitumor effect in vitro: Bioresour. Technol. 99: 3187-3194.