Purification and molecular characterization of exo-β-1,3-glucanases from the marine yeast Williopsis saturnus WC91-2

Applied Microbiology and Biotechnology

Volumn 85, Issue 1, 2009, Pages 85-94

  Peng, Ying ^a   Chi, Zhen Ming ^a   Wang, Xiang Hong ^a   Li, Jing ^a  

^a OCEAN UNIVERSITY OF CHINA   (China)

Author keywords

1,3 Glucanases; Laminarin; Marine yeast; Molecular characterization; Williopsis saturnus

Indexed keywords

ANION EXCHANGE CHROMATOGRAPHY; DNA SEQUENCING; ETHYLENE DIAMINE TETRA-ACETIC ACID; EXTRACELLULAR; GEL-FILTRATION CHROMATOGRAPHY; GLUCANASE; IODOACETIC ACID; LAMINARINS; MARINE YEASTS; MASS SPECTROSCOPY; MATRIX ASSISTED LASER DESORPTION/IONIZATION; MOLECULAR CHARACTERIZATION; PHENANTHROLINES; PHENYLMETHYLSULFONYL FLUORIDE; PURIFIED ENZYME; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; TETRAACETIC ACID; TOXIN ACTIVITY;

ACIDS; BARIUM; CELL CULTURE; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; DESORPTION; ELECTROPHORESIS; ENZYME ACTIVITY; ETHERS; ETHYLENE; ETHYLENE GLYCOL; GEL PERMEATION CHROMATOGRAPHY; GELS; GLUCOSE; MASS SPECTROMETRY; PURIFICATION; SODIUM; SODIUM SULFATE; ULTRAFILTRATION; YEAST;

ENZYMES;

1,10 PHENANTHROLINE; 1,3 BETA GLUCANASE; BARIUM; BENZYLSULFONYL FLUORIDE; EDETIC ACID; EGTAZIC ACID; GLUCAN SYNTHASE; IODOACETIC ACID; LAMINARAN; LITHIUM; NICKEL; BETA 1,3 EXOGLUCANASE; BETA-1,3-EXOGLUCANASE; DISACCHARIDE; ENZYME ACTIVATOR; ENZYME INHIBITOR; FUNGAL DNA; FUNGAL PROTEIN; GLUCAN 1,3 BETA GLUCOSIDASE; METAL; MONOSACCHARIDE; POLYSACCHARIDE;

CARBOHYDRATE; CARBOXYLIC ACID; CULTURAL ECONOMY; ELECTROKINESIS; ENZYME ACTIVITY; HOMOGENEITY; INHIBITION; IONIZATION; MASS SPECTROMETRY; MOLECULAR ANALYSIS; PH; TEMPERATURE EFFECT; ULTRAFILTRATION; YEAST;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME PURIFICATION; FUNGAL STRAIN; FUNGUS CULTURE; MARINE SPECIES; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN MOTIF; TEMPERATURE SENSITIVITY; WILLIOPSIS SATURNUS WC91-2; YEAST; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; GEL CHROMATOGRAPHY; GENETICS; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; KINETICS; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; MOLECULAR WEIGHT; SEQUENCE ALIGNMENT; TEMPERATURE; ULTRAFILTRATION; WILLIOPSIS;

WILLIOPSIS SATURNUS;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; CONSERVED SEQUENCE; DISACCHARIDES; DNA, FUNGAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATORS; ENZYME INHIBITORS; ENZYME STABILITY; FUNGAL PROTEINS; GLUCAN 1,3-BETA-GLUCOSIDASE; HYDROGEN-ION CONCENTRATION; KINETICS; METALS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; MONOSACCHARIDES; POLYSACCHARIDES; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TEMPERATURE; ULTRAFILTRATION; WILLIOPSIS;

EID: 73349138603     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-2061-1     Document Type: Article

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