Strategies for cloning PCR products

Cold Spring Harbor Protocols

Volumn 4, Issue 8, 2009, Pages

  D'Arpa, Peter ^a  

^a Howard Hughes Medical Institute Cold Spring Harbor Laboratory Cold Spring Harbor   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 73349117494     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.ip68     Document Type: Article

References (20)

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2. Hu G. 1993. DNA polymerase-catalyzed addition of nontemplated extra nucleotides to the 3′ end of a DNA fragment. DNA Cell Biol 12: 763-770.
3. Invitrogen. 2002a. pENTR Directional TOPO cloning kits (version B, July 9, 2002, 25-0434). Invitrogen Life Technologies, Inc, Rockville, MD.
4. Invitrogen. 2002b. TOPO tools technology. For generating functional constructs containing your PCR products Ad a choice of TOPO-adapted elements (version B, 072501, 25-0413). Invitrogen Life Technologies, Inc, Rockville, MD.
5. Landy A. 1989. Dynamic, structural, and regulatory aspects of lambda site-specific recombination. Annu Rev Biochem 58: 913-949.
6. Life Technologies 1999. Helpful tips for custom primers. Focus 21: 69-71.
7. Lohff CJ, Cease KB. 1992. PCR using a thermostable polymerase with 3′ to 5′ exonuclease activity generates blunt products suitable for direct cloning. Nucleic Acids Res 20: 144.
8. Mead DA, Pey NK, Herrnstadt C, Marcil RA, Smith LM. 1991. A universal method for the direct cloning,of PCR amplified nucleic acid. Bio/ Technology 9: 657-663.
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10. Rashtchian A, Buchman GW, Schuster DM, Berninger MS. 1992. Uracil DNA glycosylase mediated cloning of polymerase chain reaction-amplified DNA: Application to genomic and cDNA cloning. Anal Biochem 206: 91-97.
11. Sambrook J, Russell DW. 2006a. Purification of PCR products in preparation for cloning. Cold Spring Harb Protoc doi: 10.1101/ pdb.prot3825.
12. Sambrook J, Russell DW. 2006b. Removal of oligonucleotides an excess dNTPs from amplified DNA by ultrafiltration. Cold Spring Harb Protoc doi: 10.1101/pdb.prot3826.
13. Sambrook J, Russell DW. 2006c. Blunt-end cloning of PCR products. Cold Spring Harb Protoc doi: 10.1101/pdb/prot3830.
14. Sambrook J, Russell DW. 2006d. Cloning PCR products into T vectors. Cold Spring Harb Protoc doi: 10.1101/pdb.prot3834.
15. Sambrook J, Russell DW. 2006e. Cloning PCR products by addition of restriction sites to the termini of amplified DNA. Cold Spring Harb Protoc doi: 10.1101/pdb.prot3835.
16. Sambrook J, Russell DW. 2006f. Genetic engineering with PCR. Cold Spring Harb Protoc doi: 10.1101/pdb.prot3836.
17. Shuman S. 1994. Novel approach to molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase. J Biol Chem 269: 32678-32684.
18. Van Melderen L. 2002. Molecular interactions of the CcdB poison with its bacterial target, the DNA gyrase. Int J Med Microbiol 291: 5Y37-544.
19. Varshney U, van de Sande JH. 1991. Specificities and kinetics of uracil excision from uracil-containing DNA oligomers by Escherichia coli uracil DNA glycosylase. Biochemistry 30: 4055-4061.
20. Zimmermann K, Schogl D, Mannhalter JW. 1998. Digestion of terminal restriction endonuclease recognition sites on PCR products. BioTechniques 24: 582-584.