Digestion of terminal restriction endonuclease recognition sites on PCR products

BioTechniques

Volumn 24, Issue 4, 1998, Pages 582-584

  Zimmermann, Klaus ^a   Schögl, Daniela ^a   Mannhalter, Josef W ^a  

^a BAXTER HEALTHCARE CORPORATION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHIMERIC PROTEIN; DNA; NEF PROTEIN; RESTRICTION ENDONUCLEASE;

ARTICLE; BASE PAIRING; DNA CLEAVAGE; HUMAN IMMUNODEFICIENCY VIRUS 1; NONHUMAN; POLYMERASE CHAIN REACTION;

EID: 2642660437     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98244bm15     Document Type: Article

References (6)

1. Frohman, M.A. 1994. Cloning PCR products, p. 14-37. In K.B. Mullis, F. Ferré and R.A. Gibbs (Eds.), The Polymerase Chain Reaction, Birkhäuser, Boston.
2. Jung, V., S.B. Pestka and S. Pestka. 1993. Cloning of polymerase chain re action-generated DNA containing terminal restriction endonuclease recognition sites. Methods Enzymol. 218:357-362.
3. Jung, V., S.B. Pestka and S. Pestka. 1990. Efficient cloning of PCR generated DNA containing terminal restriction endonuclease recognition sites. Nucleic Acids Res. 18:6156.
4. Kaufman, D.L. and G.A. Evans. 1990. Restriction endonuclease cleavage at the termini of PCR products. BioTechniques 9:304-306.
5. Rychlik, W. 1995. Selection of primers for polymerase chain reaction. Mol. Biotechnol. 3:129-134.
6. Yamaguchi, K., D. Zhang and R.A. Byrn. 1994. Fluorescent primers allow direct confirmation of restriction enzyme cleavage of PCR products. BioTechniques 17:649-652.