Laser capture microdissection of human pancreatic β-cells and RNA preparation for gene expression profiling

Methods in Molecular Biology

Volumn 560, Issue , 2009, Pages 87-98

  Marselli, Lorella ^a   Sgroi, Dennis C ^a   Bonner Weir, Susan ^a   Weir, Gordon C ^a  

^a JOSLIN DIABETES CENTER   (United States)

Author keywords

Human cell; Laser capture microdissection; RNA extraction; RNA integrity; Type 2 diabetes

Indexed keywords

RNA;

ARTICLE; GENE EXPRESSION PROFILING; HUMAN; INSTRUMENTATION; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MICRODISSECTION; PANCREAS ISLET BETA CELL; TISSUE PRESERVATION;

GENE EXPRESSION PROFILING; HUMANS; INSULIN-SECRETING CELLS; MICRODISSECTION; RNA; TISSUE PRESERVATION;

EID: 73349090934     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-448-3_8     Document Type: Article

References (10)

1. Laybutt , D.R. , Kaneto , H. , Hasenkamp , W. , Grey , S. , Jonas , J.-C. , Sgroi , D.C. , Groff , A. , Ferran , C. , Bonner-Weir , S. , Sharma , A. , and Weir , G.C. (2002). Increased expression of antioxidant and antiapoptotic genes in islets that may contribute to â-cell survival during chronic hyperglycemia. Diabetes 51 , 413-423
2. Laybutt , D.R. , Sharma , A. , Sgroi , D.C. , Gaudet , J. , Bonner-Weir , S. , and Weir , G.C. (2002). Genetic regulation of metabolic pathways in â-cells disrupted by hyperglycemia. J Biol Chem 277 , 10912-10921
3. Gunton , J.E. , Kulkarni , R.N. , Yim , S. , Okada , T. , Hawthorne , W.J. , Tseng , Y.H. , Roberson , R.S. , Ricordi , C. , O'Connell , P.J. , Gonzalez , F.J. , and Kahn , C.R. (2005). Loss of ARNT/ HIF1 â mediates altered gene expression and pancreatic-islet dysfunction in human type 2 diabetes. Cell 122 , 337-349
4. Del Guerra , S. , Lupi , R. , Marselli , L. , Masini , M. , Bugliani , M. , Sbrana , S. , Torri , S. , Pollera , M. , Boggi , U. , Mosca , F. , Del Prato , S. , and Marchetti , P. (2005). Functional and molecular defects of pancreatic islets in human type 2 diabetes. Diabetes 54 , 727-735
5. Bottino , R. , Balamurugan , A.N. , Tse , H. , Thirunavukkarasu , C. , Ge , X. , Profozich , J. , Milton , M. , Ziegenfuss , A. , Trucco , M. , and Piganelli , J.D. (2004). Response of human islets to isolation stress and the effect of antioxidant treatment. Diabetes 53 , 2559-2568
6. Abdelli , S. , Ansite , J. , Roduit , R. , Borsello , T. , Matsumoto , I. , Sawada , T. , Allaman-Pillet , N. , Henry , H. , Beckmann , J.S. , Hering , B.J. , and Bonny , C. (2004). Intracellular stress signaling pathways activated during human islet preparation and following acute cytokine exposure. Diabetes 53 , 2815-2823
7. Marselli, L., Thorne, J., Ahn, Y.-B., Omer, A., Sgroi, D.C., Libermann, T., Out, H.H., Sharma, A., Bonner-Weir, S., and Weir, G.C. Gene expression of purified beta cell tissue obtained from human pancreas with laser capture microdissection. J Clin Endocrinol Metab 93 , 1046-1053
8. Bonner , R.F. , Emmert-Buck , M. , Cole , K. , Pohida , T. , Chuaqui , R. , Goldstein , S. , and Liotta , L.A. (1997). Laser capture microdissection: Molecular analysis of tissue. Science 278 , 1481-1483
9. Fend , F. , Kremer , M. , and Quintanilla-Martinez , L. (2000). Laser capture microdissection: Methodical aspects and applications with emphasis on Immuno-laser capture microdissection. Pathobiology 68 , 209-214
10. Emmert-Buck , M.R. , Bonner , R.F. , Smith , P.D. , Chuaqui , R.F. , Zhuang , Z. , Goldstein , S.R. , Weiss , R.A. , and Liotta , L.A. (1996). Laser capture microdissection. Science 274 , 998-1001