Understanding and mitigating conductivity transitions in weak cation exchange chromatography

Journal of Chromatography A

Volumn 1217, Issue 5, 2010, Pages 660-666

  Fogle, Jace ^a   Hsiung, Jenny ^a  

^a GENENTECH INC   (United States)

Author keywords

CM Ceramic HyperD; CM Sepharose Fast Flow; Conductivity transitions; Counterions; pH shift; Weak cation exchange chromatography

Indexed keywords

BUFFER CONCENTRATIONS; CM SEPHAROSE FAST FLOW; CONDUCTIVITY CHANGES; CONDUCTIVITY FLUCTUATIONS; COUNTERIONS; FLOW CONDUCTIVITY; HIGH PH; ION EXCHANGE EQUILIBRIUM; LIGAND DENSITY; PHASE DURATION; POTENTIOMETRIC ELECTRODE; PROCESS PERFORMANCE; SODIUM IONS; TARGET PROTEINS; WEAK CATION EXCHANGE CHROMATOGRAPHY;

CERAMIC MATERIALS; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; COLOR; GEL PERMEATION CHROMATOGRAPHY; ION EXCHANGE; LIGANDS; METAL IONS; POSITIVE IONS; RESINS;

PH EFFECTS;

BUFFER; COUNTERION; RESIN; SODIUM ION;

ARTICLE; CONCENTRATION (PARAMETERS); CONDUCTANCE; CONTROLLED STUDY; EFFLUENT; ION EXCHANGE CHROMATOGRAPHY; ION SELECTIVE ELECTRODE; PH; POTENTIOMETRY; PREDICTION; PRIORITY JOURNAL; PROTEIN ANALYSIS;

ANTIBODIES, MONOCLONAL; CATIONS; CERAMICS; CHROMATOGRAPHY, ION EXCHANGE; ELECTRIC CONDUCTIVITY; HYDROGEN-ION CONCENTRATION; ION EXCHANGE RESINS; SEPHAROSE; SODIUM;

EID: 73249150363     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2009.11.099     Document Type: Article

References (23)

1. Sparham S.J., and Huehns E.R. Hemoglobin 3 (1979) 13
2. Fukami Y., Hosaka Y., and Yamamoto K. FEBS Lett. 114 (1980) 342
3. Goward C.R., Stevens G.B., Collins I.J., Wilkinson I.R., and Scawen M.D. Enzyme Microb. Technol. 11 (1989) 810
4. Murao S., Nomura Y., Nagamatsu K., Hirayama K., Iwahara M., and Shin T. Agric. Biol. Chem. Tokyo 55 (1991) 1739
5. Tsao C.Y., and Nagayama F. J. Food Biochem. 15 (1991) 81
6. Baker B.C., Campbell C.J., Grinham C.J., and Turcatti G. Biochem. J. 279 (1991) 775
7. Gu Z.R., and Glatz C.E. Sep. Sci. Technol. 42 (2007) 1195
8. Tsukamoto H., Fukudome K., Kohara J., Nakatake H., and Kimoto M. Protein Expres. Purif. 56 (2007) 138
9. Necina R., Amatschek K., and Jungbauer A. Biotechnol. Bioeng. 60 (1998) 689
10. DePhillips P., and Lenhoff A.M. J. Chromatogr. 933 (2001) 57
11. Staby A., Sand M.-B., Hansen R.G., Jacobsen J.H., Andersen L.A., Gerstenberg M., Bruus U.K., and Jensen I.H. J. Chromatogr. 1069 (2005) 65
12. Staby A., Jacobsen J.H., Hansen R.G., Bruus U.K., and Jensen I.H. J. Chromatogr. 1118 (2006) 168
13. Melander W.R., El Rassi Z., and Horvath C. J. Chromatogr. 469 (1989) 3
14. Chen W.-Y., Liu Z.-C., Lin P.-H., Fang C.-I., and Yamamoto S. Sep. Purif. Technol. 54 (2007) 212
15. Karlsson E., Ryden L., and Brewer J. Protein Purification: Principles. High-Resolution Methods and Applications (1998), Wiley-VCH, New York
16. Ghose S., McNerney T.M., and Hubbard B. Biotechnol. Prog. 18 (2002) 530
17. Pabst T.M., and Carta G. J. Chromatogr. 1142 (2007) 19
18. Soto Perez J., and Frey D.D. Biotechnol. Prog. 21 (2005) 902
19. Pabst T.M., Antos D., Carta G., Ramasubramanyan N., and Hunter A.K. J. Chromatogr. 1181 (2008) 83
20. Helfferich F. Ion Exchange (1962), McGraw-Hill, New York
21. Product Note, Q, S, DEAE, CM Ceramic HyperD Ion Exchange Sorbents, Pall Corporation Life Sciences, Port Washington, New York, 2004.
22. Weaver L.E., and Carta G. Biotechnol. Prog. 12 (1996) 342
23. GE Healthcare Data File, Sepharose Fast Flow Ion Exchangers, Uppsala, Sweden, 2003.