Identification of single chain antibodies to breast cancer stem cells using phage display

Biotechnology Progress

Volumn 25, Issue 6, 2009, Pages 1780-1787

  Gur, Deniz ^a   Liu, Suling ^b   Shukla, Anurag ^a   Pero, Stephanie C ^a   Wicha, Max S ^b   Krag, David N ^a  

^a UNIVERSITY OF VERMONT COLLEGE OF MEDICINE   (United States)

^b UNIVERSITY OF MICHIGAN COMPREHENSIVE CANCER CENTER   (United States)

Author keywords

Breast; Cancer stem cell; Phage display

Indexed keywords

ADULT STEM CELLS; BREAST CANCER; BREAST CANCER CELLS; CANCER CELLS; CHEMOTHERAPEUTIC AGENTS; FROZEN TISSUES; IN-VITRO; IN-VIVO; LARGE POPULATION; NATURAL RESISTANCE; PHAGE DISPLAY; PHAGE LIBRARIES; SINGLE-CHAIN ANTIBODIES; STEM CELL; TUMOR BIOLOGY; TUMOR CELLS;

ANTIBODIES; CELL CULTURE; CELL PROLIFERATION; CLONE CELLS; ONCOLOGY; PARAFFIN WAXES; PARAFFINS; POPULATION STATISTICS; TUMORS;

STEM CELLS;

PEPTIDE LIBRARY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANIMAL; ARTICLE; BREAST TUMOR; CANCER STEM CELL; CELL ADHESION; CHEMISTRY; DRUG SCREENING; FEMALE; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; HUMAN; IMMUNOGLOBULIN VARIABLE REGION; METABOLISM; METHODOLOGY; MOUSE; MOUSE MUTANT; NONOBESE DIABETIC MOUSE; TUMOR CELL LINE;

ANIMALS; BREAST NEOPLASMS; CELL ADHESION; CELL LINE, TUMOR; FEMALE; FLOW CYTOMETRY; HUMANS; IMMUNOGLOBULIN VARIABLE REGION; MICE; MICE, INBRED NOD; MICE, SCID; MICROSCOPY, FLUORESCENCE; NEOPLASTIC STEM CELLS; PEPTIDE LIBRARY; SINGLE-CHAIN ANTIBODIES; XENOGRAFT MODEL ANTITUMOR ASSAYS;

EID: 73249148729     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1002/btpr.285     Document Type: Article

References (14)

1. Ailles LE, Weissman IL. Cancer stem cells in solid tumors. Curr Opin Biotechnol. 2007;18:460-466.
2. Dalerba PR, Cho W, Clarke MF. Cancer stem cells: models and concepts. Annu Rev Med. 2007;58:267-284.
3. Fillmore CM, Kuperwasser C. Human breast cancer cell lines contain stem-like cells that self-renew, give rise to phenotypi-cally diverse progeny and survive chemotherapy. Breast Cancer Res. 2008;10:R25.
4. Setoguchi T, Taga T, Kondo T. Cancer stem cells persist in many cancer cell lines. Cell Cycle. 2004;3:414-415.
5. Ginestier C, Hur MH, Charafe-Jauffret E, Monville F, Dutcher J, Brown M, Jacquemier J, Viens P, Kleer CO, Liu S, Schott A, Hayes D, Bimbatun D, Wicha MS, Dontu G. ALDH1 is a marker of normal and malignant human mammary stem cells and a predictor of poor clinical outcome. Cell Stem Cell. 2007; 1:555-567.
6. de Wildt RM, Mundy CR, Gorick ED, Tomlinson IM. Antibody arrays for high-throughput screening of antibody-antigen interactions. Nat Biotechnol. 2000;18:989-994.
7. Griffiths AD, Williams SC, Hartley O, Tomlinson IM, Waterhouse P, Crosby WL, Kontcrmann RE, Jones PT, Low NM, Allison TJ. Isolation of high affinity human antibodies directly from large synthetic repertoires. EMBO J. 1994;13: 3245-3260.
8. Kristensen P, Winter G. Proteolytic selection for protein fold-ing using filamentous bacteriophages. Fold Des. 1998;3:321-328.
9. Shukla GS, Krag DN. A sensitive and rapid chemiluminescence ELISA for filamentous bacteriophages. J Immunoassay Immunochem. 2005;26:89-95.
10. Liu S, Dontu G, Wicha MS. Mammary stem cells, self-renewal pathways, and carcinogenesis. Breast Cancer Res. 2005;7:86-95.
11. Shukla GS, Krag DN. Phage display selection for cell-specific ligands: development of a screening procedure suitable for small tumor specimens. J Drug Target. 2005;13:7-18.
12. Liu S, Ginestier C, Charafe-Jauffret E, Foco H, Kleer CG, Merajver SD, Dontu G, Wicha MS. BRCA1 regulates human mammary stem/progenitor cell fate. Proc Natl Acad Sci USA. 2008;105:1680-1685.
13. Ginestier C, Wicha MS. Mammary stem cell number as a determinate of breast cancer risk. Breast Cancer Res. 2007;9: 109.
14. Abraham BK, Fritz P, McClellan M, Hauptvogel P, Athelogou M, Brauch H. Prevalence of CD44{thorn}/CD24-/low cells in breast cancer may not be associated with clinical outcome but may favor distant metastasis. Clin Cancer Res. 2005;11:1154-1159.