Refolding of the integral membrane protein light-harvesting complex II monitored by pulse EPR

Proceedings of the National Academy of Sciences of the United States of America

Volumn 106, Issue 44, 2009, Pages 18485-18490

  Dockter, Christoph ^a   Volkov, Aleksei ^b   Bauer, Christian ^b   Polyhach, Yevhen ^c   Joly Lopez, Zoé ^d   Jeschke, Gunnar ^c   Paulsen, Harald ^a  

^a JOHANNES GUTENBERG UNIVERSITY   (Germany)

^b MAX PLANCK INSTITUTE FOR POLYMER RESEARCH   (Germany)

^c ETH ZURICH   (Switzerland)

^d MCGILL UNIVERSITY   (Canada)

Author keywords

Assembly kinetics; DEER; LHCII

Indexed keywords

CALNEXIN; DODECYL SULFATE;

ABSORPTION SPECTROSCOPY; ARTICLE; LIGHT HARVESTING SYSTEM; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN STABILITY; PROTEIN STRUCTURE; SPIN LABELING;

APOPROTEINS; ELECTRON SPIN RESONANCE SPECTROSCOPY; ELECTRONS; LIGHT-HARVESTING PROTEIN COMPLEXES; MEMBRANE PROTEINS; MODELS, BIOLOGICAL; MUTATION; PEAS; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; SPIN LABELS; TIME FACTORS;

EID: 73249142528     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0906462106     Document Type: Article

References (31)

1. Standfuss J, Terwisscha van Scheltinga AC, Lamborghini M, Kühlbrandt W (2005) Mechanisms of photoprotection and nonphotochemical quenching in pea lightharvesting complex at 2.5 Å resolution. EMBO J 24:919-928.
2. Paulsen H, Rümler U, Rüdiger W (1990) Reconstitution of pigment-containing complexes from light-harvesting chlorophyll-a/b-binding protein overexpressed in Escherichia coli. Planta 181:204-211.
3. Plumley FG, Schmidt GW (1987) Reconstitution of chlorophyll a/b light-harvesting complexes: Xanthophyll-dependent assembly and energy transfer. Proc Natl Acad Sci USA 84:146-150.
4. Booth PJ, Paulsen H (1996) Assembly of light-harvesting chlorophyll a/b complex in vitro. Time-resolved fluorescence measurements. Biochemistry 35:5103-5108.
5. Reinsberg D, Ottmann K, Booth PJ, Paulsen H (2001) Effects of chlorophyll a, chlorophyll b, and xanthophylls on the in vitro assembly kinetics of the major light harvesting chlorophyll a/b complex, LHCIIb. J Mol Biol 308:59-67.
6. Horn R, Paulsen H (2004) Early steps in the assembly of light-harvesting chlorophyll a/b complex. J Biol Chem 279:44400-44406.
7. Horn R, Grundmann G, Paulsen H (2007) Consecutive binding of chlorophylls a and b during the assembly in vitro of light-harvesting chlorophyll a/b protein (LHCIIb). J Mol Biol 366:1045-1054.
8. Horn R, Paulsen H (2002) Folding in vitro of light-harvesting chlorophyll a/b protein is coupled with pigment binding. J Mol Biol 318:547-556.
9. Pannier M, Veit S, Godt A, Jeschke G, Spiess HW (2000) Dead-time free measurement of dipole-dipole interactions between electron spins. J Magn Reso 142:331-340.
10. Jeschke G, Polyhach Y (2007) Distance measurements on spin-labeled biomacromolecules by pulsed electron paramagnetic resonance. Phys Chem Chem Phys 9:1895-1910.
11. Jeschke G, et al. (2005) Localization of the N-terminal domain in light-harvesting chlorophyll a/b protein by EPR measurements. J Biol Chem 280:18623-18630.
12. Hilger D, Polyhach Y, Jung H, Jeschke G (2009) Backbone structure of transmembrane domain IX of the Na+/Proline Transporter PutP of Escherichia coli. Biophys J 96:217-225.
13. Altenbach C, Kusnetzow AK, Ernst OP, Hofmann KP, Hubbell WL (2008) Highresolution distance mapping in rhodopsin reveals the pattern of helix movement due to activation. Proc Natl Acad Sci USA 105:7439-7444.
14. Klein JC, et al. (2008) Actin-binding cleft closure in myosin II probed by site-directed spin labeling and pulsed EPR. Proc Natl Acad Sci USA 105:12867-12872.
15. Volkov A, Dockter C, Bund T, Paulsen H, Jeschke G (2009) Pulsed EPR determination of water accessibility to spin-labeled amino acid residues in LHCIIb. Biophys J 96:1124-1141.
16. Farahbakhsh ZT, Hideg K, Hubbell WL (1993) Photoactivated conformational changes in rhodopsin: A time-resolved spin label study. Science 262:1416-1419.
17. Shin YK, Levinthal C, Levinthal F, Hubbell WL (1993) Colicin E1 binding to membranes: Time-resolved studies of spin-labeled mutants. Science 259:960-963.
18. Steinhoff HJ, et al. (1994) Time-resolved detection of structural changes during the photocycle of spin-labeled bacteriorhodopsin. Science 266:105-107.
19. DeWeerd K, Grigoryants V, Sun Y, Fetrow JS, Scholes CP (2001) EPR-detected folding kinetics of externally located cysteine-directed spin-labeled mutants of iso-1-cytochrome c. Biochemistry 40:15846-15855.
20. Hobe S, Niemeier H, Bender A, Paulsen H (2000) Carotenoid binding sites in LHCIIb. Relative affinities towards major xanthophylls of higher plants. Eur J Biochem 267:616-624.
21. Jeschke G, et al. (2006) DeerAnalysis2006 - A comprehensive software package for analyzing pulsed ELDOR data. Appl Magn Reson 30:473-498.
22. Reinsberg D, Booth PJ, Jegerschoeld C, Khoo BJ, Paulsen H (2000) Folding, assembly, and stability of the major light-harvesting complex of higher plants, LHCII, in the presence of native lipids. Biochemistry 39:14305-14313.
23. Hobe S, Prytulla S, Kühlbrandt W, Paulsen H (1994) Trimerization and crystallization of reconstituted light-harvesting chlorophyll a/b complex. EMBO J 13:3423-3429.
24. Ibel K, et al. (1994) Structure of dodecyl sulfate-protein complexes at subsaturating concentrations of free detergent. Biophys Chem 53:77-84.
25. Booth PJ, Curnow P (2006) Membrane proteins shape up: Understanding in vitro folding. Curr Opin Struct Biol 16:480-488.
26. Yang C, Horn R, Paulsen H (2003) The light-harvesting chlorophyll a/b complex can be reconstituted in vitro from its completely unfolded apoprotein. Biochemistry 42:4527-4533.
27. Paulsen H, Finkenzeller B,Kühlein N (1993) Pigments induce folding of light-harvesting chlorophyll a/b-binding protein. Eur J Biochem 215:809-816.
28. Popot JL, Engelman DM (1990) Membrane protein folding and oligomerization: The two-stage model. Biochemistry 29:4031-4037.
29. Popot JL, Engelman DM (2000) Helical membrane protein folding, stability, and evolution. Annu Rev Biochem 69:881-922.
30. Engelman DM, et al. (2003) Membrane protein folding: Beyond the two stage model. FEBS Lett 555:122-125.
31. Cashmore AR (1984) Structure and expression of a pea nuclear gene encoding a chlorophyll a/b-binding polypeptide. Proc Natl Acad Sci USA 81:2960-2964.