Folding in vitro of light-harvesting chlorophyll a/b protein is coupled with pigment binding

Journal of Molecular Biology

Volumn 318, Issue 2, 2002, Pages 547-556

  Horn, Ruth ^a   Paulsen, Harald ^a  

^a JOHANNES GUTENBERG UNIVERSITY   (Germany)

Author keywords

Assembly; Circular dichroism; Photosynthesis; Pigment protein complex; Stopped flow kinetics

Indexed keywords

APOPROTEIN; CAROTENOID; CHLOROPHYLL A; CHLOROPHYLL B; DODECYL SULFATE; PIGMENT;

ALPHA HELIX; ARTICLE; CIRCULAR DICHROISM; ENERGY TRANSFER; KINETICS; LIGHT HARVESTING SYSTEM; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN SECONDARY STRUCTURE; SOLUBILITY; ULTRAVIOLET RADIATION;

EID: 0036301968     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-2836(02)00115-8     Document Type: Article

References (43)

1. Reconstitution of pigment-containing complexes from light-harvesting chlorophyll a/b-binding protein overexpressed in E. coli
2. In-vitro reconstitution of a light-harvesting gene product: Deletion mutagenesis and analyses of pigment binding
3. Reconstitution of chlorophyll a/b light-harvesting complexes: Xanthophyll-dependent assembly and energy transfer
4. Assembly of light-harvesting chlorophyll a/b complex in vitro
5. Folding, assembly, and stability of the major light-harvesting complex of higher plants, LHCII, in the presence of native lipids
6. Pigments induce folding of light-harvesting chlorophyll a/b-binding protein
7. A self-consistent method for the analysis of protein secondary structure from circular dichroism
8. Exchange of pigment-binding amino acids in light-harvesting chlorophyll a/b protein
9. Effects of chlorophyll a, chlorophyll b, and xanthophylls on the in vitro assembly kinetics of the major light-harvesting chlorophyll a/b complex, LHCIIb
10. A new basis for interpreting the circular dichroic spectra of proteins
11. Atomic model of plant light-harvesting complex by electron crystallography
12. Pigment-binding properties of mutant light-harvesting chlorophyll a/b-binding protein
13. Extending CD spectra of proteins to 168 nm improves the analysis for secondary structures
14. Slow a helix formation during folding of a membrane protein
15. Secondary structure of bacteriorhodopsin fragments - External sequence constraints specify the conformation of transmembrane helices
16. A method for assessing the stability of a membrane protein
17. Refolding of an integral membrane protein. Denaturation, renaturation, and reconstitution of intact bacteriorhodopsin and two proteolytic fragments
18. Kinetics of folding and membrane insertion of a beta-barrel membrane protein
19. Folding and membrane insertion of the trimeric β-barrel protein OmpF
20. Outer membrane protein A of Escherichia coli inserts and folds into lipid bilayers by a concerted mechanism
21. Characterization of the slow folding reactions of trp aporepressor from Escherichia coli by mutational analysis of prolines and catalysis by a peptidyl-prolyl isomerase
22. Prolyl isomerase: Enzymatic catalysis of slow protein-folding reactions
23. Kinetic measurement of cytochrome c folding: Involvement of the heme and its ligands
24. Proline residues in transmembrane α helices affect the folding of bacteriorhodopsin
25. Proton NMR investigation of te reconstitution of equine myoglobin and hemin dicyanide. Evidence for the late formation of the proximal His93 F8-iron bond
26. Kinetic study on myoglobin refolding monitored by five optical probe stopped-flow methods
27. Intermediates in the folding of the membrane protein bacteriorhodopsin
28. A biophysical study of integral membrane protein folding
29. Pigment and protein composition of reconstituted light-harvesting complexes and effects of some protein modifications
30. Membrane protein folding and oligomerization: The two-stage model
31. Helical membrane protein folding, stability, and evolution
32. Random mutations directed to trans-membrane and loop domains of light-harvesting chlorophyll a/b protein: Impact on pigment binding
33. Trimerization and crystallization of reconstituted light-harvesting chlorophyll a/b complex
34. Biosynthesis of the light-harvesting chlorophyll a/b protein. Polypeptide turnover in darkness
35. Light harvesting chlorophyll a/b binding protein stably inserts into etioplast membranes supplemented with Zn pheophytin a/b
36. Assembly of light-harvesting complex II and biogenesis of thylakoid membranes in chloroplasts
37. Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: Verfication of the concentration of chlorophyll standards by atomic absorption spectroscopy
38. Carotenoids
39. Measurement of protein using bicinchoninic acid
40. Determination of the aggregate size in detergent solution of the light-harvesting chlorophyll a/b-protein complex from chloroplast membranes
41. Differential light scattering and absorption flattening optical effects are minimal in the circular dichroism spectra of small unilamellar vesicles
42. Dictionary of protein secondary structure: Pattern recognition of hydrogen-bonded and geometrical features
43. Estimation of protein secondary structure from circular dichroism spectra: Inclusion of denatured proteins with native proteins in the analysis