Paper-supported 3D cell culture for tissue-based bioassays

Proceedings of the National Academy of Sciences of the United States of America

Volumn 106, Issue 44, 2009, Pages 18457-18462

  Derda, Ratmir ^a,b   Laromaine, Anna ^a   Mammoto, Akiko ^c   Tang, Sindy K Y ^a   Mammoto, Tadanori ^c   Ingber, Donald E ^b,c,d   Whitesides, George M ^a,b  

^a HARVARD UNIVERSITY   (United States)

^b HARVARD UNIVERSITY   (United States)

^c BOSTON CHILDREN S HOSPITAL   (United States)

^d HARVARD UNIVERSITY   (United States)

Author keywords

Diffusion; Hypoxia; Molecular gradients; Multilayer constructs

Indexed keywords

ANIMAL CELL; APOPTOSIS; ARTICLE; BIOASSAY; CANCER CELL CULTURE; CELL PROLIFERATION; CELL SUSPENSION; CELL VIABILITY; CELLULAR DISTRIBUTION; CONTROLLED STUDY; EXTRACELLULAR MATRIX; GAS PERMEABILITY; GEOMETRY; HYDROGEL; HYPOXIA; MOUSE; NONHUMAN; NUTRIENT; OXYGEN CONCENTRATION; PAPER; PRIORITY JOURNAL; THREE DIMENSIONAL IMAGING; TUMOR SPHEROID;

ANIMALS; BIOLOGICAL ASSAY; CELL CULTURE TECHNIQUES; CELL LINE; CELL SEPARATION; CELL SURVIVAL; GENE EXPRESSION REGULATION; HUMANS; HYDROGELS; MICE; OXYGEN; PAPER; PERMEABILITY;

EID: 73249131798     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0910666106     Document Type: Article

References (24)

1. Minchinton AI, Tannock IF (2006) Drug penetration in solid tumours. Nat Rev Cancer 6:583-592.
2. Malda J, Klein TJ, Upton Z (2007) The roles of hypoxia in the In vitro engineering of tissues. Tissue Eng Part A 13:2153-2162.
3. YamadaKM,Cukierman E (2007) Modeling tissue morphogenesis and cancer in 3D. Cell 130:601-610.
4. Schmeichel KL, Bissell MJ (2003) Modeling tissue-specific signaling and organ function in three dimensions. J Cell Sci 116:2377-2388.
5. Pampaloni F, Reynaud EG, Stelzer EHK (2007) The third dimension bridges the gap between cell culture and live tissue. Nat Rev Mol Cell Biol 8:839-845.
6. Folkman J, Hochberg M (1973) Self-regulation of growth in 3 dimensions. J Exp Med 138:745-753.
7. Thomlinson RH, Gray LH (1955) The histological structure of some human lung cancers and the possible implications for radiotherapy. Br J Cancer 9:539-549.
8. Vaupel P, Kallinowski F, Okunieff P (1989) Blood-flow, oxygen and nutrient supply, and metabolic microenvironment of human-tumors-a review. Cancer Res 49:6449 - 6465.
9. McGuigan AP, Sefton MV (2006) Vascularized organoid engineered by modular assembly enables blood perfusion. Proc Natl Acad Sci USA 103:11461-11466.
10. Nelson CM, Inman JL, Bissell MJ (2008) Three-dimensional lithographically defined organotypic tissue arrays for quantitative analysis of morphogenesis and neoplastic progression. Nat Protoc 3:674-678.
11. Albrecht DR, Underhill GH, Wassermann TB, Sah RL, Bhatia SN (2006) Probing the role of multicellular organization in three-dimensional microenvironments. Nat Methods 3:369-375.
12. Griffith LG, SwartzMA(2006) Capturing complex 3D tissue physiology in vitro. Nat Rev Mol Cell Biol 7:211-224.
13. Ferreira LFV, Freixo MR, Garcia AR, Wilkinson F (1992) Photochemistry on surfaces-fluorescence emission quantum yield evaluation of dyes adsorbed on microcrystalline cellulose. J Chem Soc Faraday Trans 88:15-22.
14. Martin Y, Vermette P (2005) Bioreactors for tissue mass culture: Design, characterization, and recent advances. Biomaterials 26:7481-7503.
15. Eguchi R, Suzuki A, Miyakaze S, Kaji K, Ohta T (2007) Hypoxia induces apoptosis of HUVECs in an in vitro capillary model by activating proapoptotic signal p38 through suppression of ERK1/2. Cell Signal 19:1121-1131.
16. Durand RE (1982) Use of Hoechst-33342 for cell selection from multicell systems. J Histochem Cytochem 30:117-122.
17. Schofield CJ, Ratcliffe PJ (2004) Oxygen sensing by HIF hydroxylases. Nat Rev Mol Cell Biol 5:343-354.
18. Stern SA, Shah VM, Hardy BJ (1987) Structure-permeability Relationships in silicone polymers. J Polym Sci Pol Phys 25:1263-1298.
19. Helmlinger G, Yuan F, Dellian M, Jain RK (1997) Interstitial pH and pO(2) gradients in solid tumorsinvivo:High- resolutionmeasurementsrevealalackofcorrelation.NatMed3:177-182.
20. Carrilho E, Phillips ST, Vella SJ, Martinez AW, Whitesides GM (2009) Paper microzone plates. Anal Chem 81:5990-5998.
21. Frank R (1992) Spot-synthesis-an easy technique for the positionally addressable, parallel chemical synthesis on a membrane support. Tetrahedron 48:9217-9232.
22. Blackwell HE (2006) Hitting the SPOT: small-molecule macroarrays advance combinatorial synthesis. Curr Opin Chem Biol 10:203-212.
23. Bowman MD, O'Neill JC, Stringer JR, Blackwell HE (2007) Rapid identification of antibacterial agents effective against Staphylococcus aureus using small-molecule macroarrays. Chem Biol 14:351-357.
24. Kleinman HK et al. (1986) Basement-membrane complexes with biological-activity. Biochemistry 25:312-318.