Knockdown of the myostatin gene by RNA interference in caprine fibroblast cells

Journal of Biotechnology

Volumn 145, Issue 2, 2010, Pages 99-102

  Jain, Hemlata ^a   Singh, Sanjeev ^a   Kadam, Megha ^a   Sarkhel, B C ^a  

^a Biotechnology Centre   (India)

Author keywords

Caprine fibroblasts; Knockdown; Myostatin; Real time RT PCR; RNA interference; Short hairpin RNA

Indexed keywords

DOWN-REGULATION; FIBROBLAST CELLS; GENE KNOCK DOWN; GENETIC MANIPULATIONS; LONG TERM; MAMMALIAN SPECIES; MRNA LEVEL; MUSCLE CELL; MUSCLE MASS; MYOBLAST CELLS; MYOSTATIN; NEGATIVE REGULATORS; REAL TIME RT-PCR; RNA INTERFERENCE; SHORT HAIRPIN RNA; SHORT HAIRPIN RNA (SHRNA); SKELETAL MUSCLE GROWTH; STABLE EXPRESSION; TRANSFORMING GROWTH FACTORS;

BIOACTIVITY; CELL CULTURE; FIBROBLASTS; GENE EXPRESSION; MAMMALS; MUSCLE;

RNA;

INTERFERON; MESSENGER RNA; MYOSTATIN; SHORT HAIRPIN RNA; SHORT HAIRPIN RNA 1; SHORT HAIRPIN RNA 3; SHORT HAIRPIN RNA 4; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BIOTECHNOLOGICAL PROCEDURES; CONTROLLED STUDY; DOWN REGULATION; GENE EXPRESSION REGULATION; GENE SILENCING; GENE TARGETING; GENETIC TRANSFECTION; GOAT; KNOCKOUT GENE; MUSCLE CELL; MUSCLE GROWTH; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; REAL TIME POLYMERASE CHAIN REACTION; RNA INTERFERENCE; RNA SEQUENCE; SKIN FIBROBLAST; STABLE EXPRESSION; UPREGULATION;

ANIMALS; CELL PROLIFERATION; CELLS, CULTURED; DOWN-REGULATION; FIBROBLASTS; GENETIC ENHANCEMENT; GOATS; MYOSTATIN; PROTEIN ENGINEERING; RNA INTERFERENCE;

CAPRA; MAMMALIA;

EID: 73149120715     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2009.10.017     Document Type: Article

References (23)

1. Adams A.M., Pratt S.L., and Stice S.L. Knockdown of the Dnmt1 transcript using small interfering RNA in primary murine and bovine fibroblast cells. Mol. Reprod. Dev. 72 (2005) 311-319
2. Bartel D.P. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116 (2004) 281-297
3. Forbes D., Jackman M., Bishop A., Thomas M., Kambadur R., and Sharma M. Myostatin auto-regulates its expression by feedback loop through Smad7 dependent mechanism. J. Cell. Physiol. 206 (2006) 264-272
4. Golding M.C., Long C.R., Carmell M.A., Hannon G.J., and Westhusin M.E. Suppression of prion protein in livestock by RNA interference. Proc. Natl. Acad. Sci. U.S.A. 103 14 (2006) 5285-5290
5. Langley B., Thomas M., Bishop A., Sharma M., Gilmour S., and Kambadur R. Myostatin inhibits myoblast differentiation by down-regulating myod expression. J. Biol. Chem. 277 (2002) 49831-49840
6. Livek K.J., and Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method. Methods 25 (2001) 402-408
7. Ma K., Mallidis C., Artaza J., Taylor W., Gonzalez-Cadavid N., and Bhasin S. Characterization of 5′-regulatory region of human myostatin gene regulation by dexamethasone in vitro. Am. J. Physiol. Endocrinol. Metab. 281 (2001) E1128-E1136
8. Magee T.R., Artaza J.N., Ferrini M.G., Vernet D., Zuniga F.I., Cantini L., Reiszporszasz S., Rejfer J., and Gonzalez-cavadid N.F. Myostatin short interfering hairpin RNA gene transfer increase skeletal muscle mass. J. Gene Med. 8 (2006) 1171-1181
9. McCaffrey A.P., Meuse L., Pham T.T., Conklin D.S., Hannon G.J., and Kay M.A. RNA interference in adult mice. Nature 418 (2000) 38-39
10. McPherron A.C., Lawler A.M., and Lee S.J. Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member. Nature 387 (1997) 83-90
11. McPherron A.C., and Lee S.J. Double muscling in cattle due to mutation in the myostatin gene. Proc. Nati. Acad. Sci. 94 (1997) 12457-12461
12. Miyagishi M., Sumimoto H., Miyoshi H., Kawakami Y., and Taira K. Optimization of an siRNA-expression system with an improved hairpin and its significant suppressive effects in mammalian cells. J. Gene Med. 6 (2004) 715-723
13. Muller U., Steinhoff U., Reis L.F., Hemmi S., Pavlovic J., Zinkernagel R.M., and Aguet M. Functional role of type I and type II interferons in antiviral defense. Science 264 (1994) 1918-1921
14. Patzel V., Rutz S., Dietrich I., Koberle C., Scheffold A., and Kaufmann S.H. Design of siRNAs producing unstructured guide-RNAs results in improved RNA interference efficiency. Nat. Biotechnol. 23 (2005) 1440-1444
15. Radaelli G., Rowlerson A., Mascarello F., Patruno M., and Funkenstein B. Myostatin precursor is present in several tissues in teleost fish: a comparative immunolocalization study. Cell Tissue Res. 311 (2003) 239-250
16. Reynolds A., Leake D., Boese Q., Scaringe S., Marshall W.S., and Khvorova A. Rational siRNA design for RNA interference. Nat. Biotechnol. 22 (2004) 326-330
17. Sambrook J., and Russell D.W. Molecular Cloning: A Laboratory Manual (2001), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA
18. Sato F., Kurokawa M., Yamauchi N., and Hattori M.A. Gene silencing of myostatin in differentiation of chicken embryonic myoblasts by small interfering RNA. Am. J. Physiol. Cell Physiol. 291 (2006) C538-C545
19. Scherer L.J., and Rossi J.J. Approaches for the sequence-specific knockdown of mRNA. Nat. Biotechnol. 21 (2003) 1457-1465
20. Spiller M.P., Kambadur R., Jeanplong F., Thomas M., Martyn J.K., Bass J.J., and Sharma M. The Myostatin gene is downstream target gene of basic helix-loop-helix transcription factor MyoD. Mol. Cell. Biol. 22 20 (2002) 7066-7082
21. Stewart, C.K., 2006. Presence of RNA interference in fetal porcine fibroblasts. M.Sc. Thesis submitted to the University of Guelph, Canada.
22. Stewart C.K., Li J., and Golovan S.P. Adverse effects induced by short hairpin RNA expression in porcine fetal fibroblasts. Biochem. Biophys. Res. Commun. 370 (2008) 113-117
23. Wiener P., Smith J.A., Lewis A.M., Woolliams J.A., and Williams J.L. Muscle-related traits in cattle: the role of the myostatin gene in the South Devon breed. Genet. Sel. Evol. 34 (2002) 221-232