Cross-contamination during processing of dried blood spots used for rapid diagnosis of HIV-1 infection of infants is rare and avoidable

Journal of Virological Methods

Volumn 163, Issue 2, 2010, Pages 489-491

  Mitchell, Caroline ^a   Kraft, Kelli ^c   Peterson, Do ^c   Frenkel, Lisa ^a,b,c  

^a UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF WASHINGTON   (United States)

^c SEATTLE CHILDREN S HOSPITAL   (United States)

Author keywords

Dried blood spots; Infant HIV diagnosis; PCR contamination

Indexed keywords

POL PROTEIN; VIRUS DNA;

ARTICLE; BLOOD SAMPLING; CONTROLLED STUDY; DIAGNOSTIC PROCEDURE; DIAGNOSTIC TEST; DRIED BLOOD SPOT; FALSE POSITIVE RESULT; FILTER PAPER; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1 INFECTION; INFANT; INFANT DISEASE; NESTED POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEIC ACID AMPLIFICATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; TEST STRIP; VIRAL CONTAMINATION; VIRUS CONCENTRATION; VIRUS DETECTION;

BLOOD; DESICCATION; DNA, VIRAL; FALSE POSITIVE REACTIONS; HIV INFECTIONS; HIV-1; HUMANS; INFANT; INFANT, NEWBORN; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPECIMEN HANDLING;

HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 73049114174     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2009.10.016     Document Type: Article

References (8)

1. Beck I.A., Drennan K.D., Melvin A.J., Mohan K.M., Herz A.M., Alarcon J., Piscoya J., Velazquez C., and Frenkel L.M. Simple, sensitive, and specific detection of human immunodeficiency virus type 1 subtype B DNA in dried blood samples for diagnosis in infants in the field. J. Clin. Microbiol. 39 (2001) 29-33
2. Cassol S.A., Read S., Weniger B.G., Gomez P., Lapointe N., Ou C.Y., and Babu P.G. Dried blood spots collected on filter paper: an international resource for the diagnosis and genetic characterization of human immunodeficiency virus type-1. Mem. Inst. Oswaldo Cruz 91 (1996) 351-358
3. Driver G.A., Patton J.C., Moloi J., Stevens W.S., and Sherman G.G. Low risk of contamination with automated and manual excision of dried blood spots for HIV DNA PCR testing in the routine laboratory. J. Virol. Methods 146 (2007) 397-400
4. Li C.C., Beck I.A., Seidel K.D., and Frenkel L.M. Persistence of human immunodeficiency virus type 1 subtype B DNA in dried-blood samples on FTA filter paper. J. Clin. Microbiol. 42 (2004) 3847-3849
5. Mitchell C., Jennings C., Brambilla D., Aldrovandi G., Amedee A.M., Beck I., Bremer J.W., Coombs R., Decker D., Fiscus S., Fitzgibbon J., Luzuriaga K., Moye J., Palumbo P., Reichelderfer P., Somasundaran M., Stevens W., and Frenkel L. Diminished human immunodeficiency virus type 1 DNA yield from dried blood spots after storage in a humid incubator at 37 degrees C compared to -20 degrees C. J. Clin. Microbiol. 46 (2008) 2945-2949
6. UNAIDS, 2007. UNAIDS Website.
7. Violari A., Cotton M.F., Gibb D.M., Babiker A.G., Steyn J., Madhi S.A., Jean-Philippe P., and McIntyre J.A. Early antiretroviral therapy and mortality among HIV-infected infants. N. Engl. J. Med. 359 (2008) 2233-2244
8. Youngpairoj A.S., Masciotra S., Garrido C., Zahonero N., de Mendoza C., and Garcia-Lerma J.G. HIV-1 drug resistance genotyping from dried blood spots stored for 1 year at 4 degrees C. J. Antimicrob. Chemother. 61 (2008) 1217-1220